Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS02535 and HSERO_RS02540 are separated by 66 nucleotides HSERO_RS02540 and HSERO_RS02545 are separated by 82 nucleotides HSERO_RS02545 and HSERO_RS02550 are separated by 110 nucleotides
HSERO_RS02535: HSERO_RS02535 - short-chain dehydrogenase, at 557,829 to 558,578
_RS02535
HSERO_RS02540: HSERO_RS02540 - ABC transporter substrate-binding protein, at 558,645 to 559,682
_RS02540
HSERO_RS02545: HSERO_RS02545 - membrane protein, at 559,765 to 560,391
_RS02545
HSERO_RS02550: HSERO_RS02550 - protamine P1 domain containing protein, at 560,502 to 560,711
_RS02550
Position (kb) 558
559
560 Strain fitness (log2 ratio) -1
0
1
2 at 557.793 kb on - strand at 558.051 kb on + strand, within HSERO_RS02535 at 558.216 kb on + strand, within HSERO_RS02535 at 558.226 kb on + strand, within HSERO_RS02535 at 558.444 kb on + strand, within HSERO_RS02535 at 558.642 kb on - strand at 558.665 kb on + strand at 558.783 kb on + strand, within HSERO_RS02540 at 558.791 kb on - strand, within HSERO_RS02540 at 558.791 kb on - strand, within HSERO_RS02540 at 558.961 kb on - strand at 559.041 kb on + strand, within HSERO_RS02540 at 559.226 kb on + strand, within HSERO_RS02540 at 559.328 kb on + strand, within HSERO_RS02540 at 559.333 kb on + strand, within HSERO_RS02540 at 559.351 kb on - strand, within HSERO_RS02540 at 559.441 kb on - strand, within HSERO_RS02540 at 559.509 kb on + strand, within HSERO_RS02540 at 559.538 kb on - strand, within HSERO_RS02540 at 559.582 kb on + strand at 559.676 kb on + strand at 559.767 kb on + strand at 560.224 kb on - strand, within HSERO_RS02545 at 560.274 kb on - strand, within HSERO_RS02545 at 560.378 kb on - strand at 560.406 kb on + strand at 560.414 kb on - strand at 560.414 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 557,793 - +0.2 558,051 + HSERO_RS02535 0.30 +2.2 558,216 + HSERO_RS02535 0.52 +0.1 558,226 + HSERO_RS02535 0.53 -0.1 558,444 + HSERO_RS02535 0.82 +0.3 558,642 - -0.5 558,665 + +0.1 558,783 + HSERO_RS02540 0.13 -0.3 558,791 - HSERO_RS02540 0.14 +0.7 558,791 - HSERO_RS02540 0.14 +0.1 558,961 - +0.6 559,041 + HSERO_RS02540 0.38 -0.1 559,226 + HSERO_RS02540 0.56 +2.0 559,328 + HSERO_RS02540 0.66 -0.0 559,333 + HSERO_RS02540 0.66 +0.2 559,351 - HSERO_RS02540 0.68 +0.3 559,441 - HSERO_RS02540 0.77 +0.8 559,509 + HSERO_RS02540 0.83 +0.2 559,538 - HSERO_RS02540 0.86 +0.7 559,582 + -0.3 559,676 + -0.5 559,767 + -0.4 560,224 - HSERO_RS02545 0.73 +0.3 560,274 - HSERO_RS02545 0.81 -0.2 560,378 - -1.2 560,406 + +0.9 560,414 - +0.6 560,414 - +0.2
Or see this region's nucleotide sequence