Experiment: KB with Ferulic Acid 180 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2745 and Psyr_2746 are separated by 5 nucleotides Psyr_2746 and Psyr_2747 are separated by 44 nucleotides
Psyr_2745: Psyr_2745 - Pyridoxal-5'-phosphate-dependent enzyme, beta subunit:Ornithine cyclodeaminase/mu-crystallin, at 3,331,714 to 3,333,771
_2745
Psyr_2746: Psyr_2746 - L-lysine 6-monooxygenase, putative, at 3,333,777 to 3,335,096
_2746
Psyr_2747: Psyr_2747 - amino acid/amide ABC transporter substrate-binding protein, HAAT family, at 3,335,141 to 3,336,121
_2747
Position (kb)
3333
3334
3335
3336 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3332.876 kb on + strand, within Psyr_2745 at 3333.266 kb on + strand, within Psyr_2745 at 3333.266 kb on + strand, within Psyr_2745 at 3333.266 kb on + strand, within Psyr_2745 at 3333.267 kb on - strand, within Psyr_2745 at 3333.267 kb on - strand, within Psyr_2745 at 3333.345 kb on - strand, within Psyr_2745 at 3333.362 kb on + strand, within Psyr_2745 at 3333.363 kb on - strand, within Psyr_2745 at 3333.464 kb on + strand, within Psyr_2745 at 3333.767 kb on + strand at 3333.767 kb on + strand at 3333.767 kb on + strand at 3333.767 kb on + strand at 3333.767 kb on + strand at 3333.767 kb on + strand at 3333.767 kb on + strand at 3333.767 kb on + strand at 3333.767 kb on + strand at 3333.767 kb on + strand at 3333.767 kb on + strand at 3333.768 kb on - strand at 3333.768 kb on - strand at 3333.768 kb on - strand at 3333.768 kb on - strand at 3333.768 kb on - strand at 3333.768 kb on - strand at 3333.768 kb on - strand at 3333.768 kb on - strand at 3333.817 kb on - strand at 3333.926 kb on + strand, within Psyr_2746 at 3334.168 kb on + strand, within Psyr_2746 at 3334.168 kb on + strand, within Psyr_2746 at 3334.168 kb on + strand, within Psyr_2746 at 3334.169 kb on - strand, within Psyr_2746 at 3334.169 kb on - strand, within Psyr_2746 at 3334.175 kb on - strand, within Psyr_2746 at 3334.175 kb on - strand, within Psyr_2746 at 3334.175 kb on - strand, within Psyr_2746 at 3334.213 kb on + strand, within Psyr_2746 at 3334.214 kb on - strand, within Psyr_2746 at 3334.261 kb on + strand, within Psyr_2746 at 3334.261 kb on + strand, within Psyr_2746 at 3334.262 kb on - strand, within Psyr_2746 at 3334.262 kb on - strand, within Psyr_2746 at 3334.324 kb on + strand, within Psyr_2746 at 3334.324 kb on + strand, within Psyr_2746 at 3334.324 kb on + strand, within Psyr_2746 at 3334.651 kb on - strand, within Psyr_2746 at 3334.731 kb on + strand, within Psyr_2746 at 3334.731 kb on + strand, within Psyr_2746 at 3334.757 kb on - strand, within Psyr_2746 at 3334.831 kb on + strand, within Psyr_2746 at 3334.831 kb on + strand, within Psyr_2746 at 3334.831 kb on + strand, within Psyr_2746 at 3334.832 kb on - strand, within Psyr_2746 at 3334.832 kb on - strand, within Psyr_2746 at 3334.832 kb on - strand, within Psyr_2746 at 3334.832 kb on - strand, within Psyr_2746 at 3334.887 kb on + strand, within Psyr_2746 at 3334.887 kb on + strand, within Psyr_2746 at 3334.888 kb on - strand, within Psyr_2746 at 3334.888 kb on - strand, within Psyr_2746 at 3335.111 kb on + strand at 3335.112 kb on - strand at 3335.122 kb on + strand at 3335.142 kb on + strand at 3335.320 kb on - strand, within Psyr_2747 at 3335.320 kb on - strand, within Psyr_2747 at 3335.320 kb on - strand, within Psyr_2747 at 3335.320 kb on - strand, within Psyr_2747 at 3335.346 kb on + strand, within Psyr_2747 at 3335.346 kb on + strand, within Psyr_2747 at 3335.346 kb on + strand, within Psyr_2747
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ferulic Acid 180 ug/ml remove 3,332,876 + Psyr_2745 0.56 +0.1 3,333,266 + Psyr_2745 0.75 -1.4 3,333,266 + Psyr_2745 0.75 +0.3 3,333,266 + Psyr_2745 0.75 +1.3 3,333,267 - Psyr_2745 0.75 -1.1 3,333,267 - Psyr_2745 0.75 -0.3 3,333,345 - Psyr_2745 0.79 -1.1 3,333,362 + Psyr_2745 0.80 +1.0 3,333,363 - Psyr_2745 0.80 -1.1 3,333,464 + Psyr_2745 0.85 +0.7 3,333,767 + +0.4 3,333,767 + +0.4 3,333,767 + +1.6 3,333,767 + +0.0 3,333,767 + -0.6 3,333,767 + +0.0 3,333,767 + -0.0 3,333,767 + +0.1 3,333,767 + -0.6 3,333,767 + -0.1 3,333,767 + +0.2 3,333,768 - +1.0 3,333,768 - +0.5 3,333,768 - +2.2 3,333,768 - +0.3 3,333,768 - +1.2 3,333,768 - -0.4 3,333,768 - -1.4 3,333,768 - -2.6 3,333,817 - +1.1 3,333,926 + Psyr_2746 0.11 -0.1 3,334,168 + Psyr_2746 0.30 -0.5 3,334,168 + Psyr_2746 0.30 -0.4 3,334,168 + Psyr_2746 0.30 -0.6 3,334,169 - Psyr_2746 0.30 -0.0 3,334,169 - Psyr_2746 0.30 -2.4 3,334,175 - Psyr_2746 0.30 -0.1 3,334,175 - Psyr_2746 0.30 -0.1 3,334,175 - Psyr_2746 0.30 -2.1 3,334,213 + Psyr_2746 0.33 -0.9 3,334,214 - Psyr_2746 0.33 -1.1 3,334,261 + Psyr_2746 0.37 +0.0 3,334,261 + Psyr_2746 0.37 -0.5 3,334,262 - Psyr_2746 0.37 +1.8 3,334,262 - Psyr_2746 0.37 +1.4 3,334,324 + Psyr_2746 0.41 +0.3 3,334,324 + Psyr_2746 0.41 +0.3 3,334,324 + Psyr_2746 0.41 -0.3 3,334,651 - Psyr_2746 0.66 -4.1 3,334,731 + Psyr_2746 0.72 +0.1 3,334,731 + Psyr_2746 0.72 +0.4 3,334,757 - Psyr_2746 0.74 +0.2 3,334,831 + Psyr_2746 0.80 -1.6 3,334,831 + Psyr_2746 0.80 -0.6 3,334,831 + Psyr_2746 0.80 -1.6 3,334,832 - Psyr_2746 0.80 -0.6 3,334,832 - Psyr_2746 0.80 +1.4 3,334,832 - Psyr_2746 0.80 -0.9 3,334,832 - Psyr_2746 0.80 +3.4 3,334,887 + Psyr_2746 0.84 +0.2 3,334,887 + Psyr_2746 0.84 -0.6 3,334,888 - Psyr_2746 0.84 -3.1 3,334,888 - Psyr_2746 0.84 -0.1 3,335,111 + +1.1 3,335,112 - -0.2 3,335,122 + +0.9 3,335,142 + -0.9 3,335,320 - Psyr_2747 0.18 -0.1 3,335,320 - Psyr_2747 0.18 -0.3 3,335,320 - Psyr_2747 0.18 -2.4 3,335,320 - Psyr_2747 0.18 +0.2 3,335,346 + Psyr_2747 0.21 +1.6 3,335,346 + Psyr_2747 0.21 +1.7 3,335,346 + Psyr_2747 0.21 +0.2
Or see this region's nucleotide sequence