Experiment: KB with Ferulic Acid 180 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1213 and Psyr_1214 overlap by 4 nucleotides Psyr_1214 and Psyr_1215 are separated by 33 nucleotides
Psyr_1213: Psyr_1213 - type III secretion cytoplasmic ATPase HrcN, at 1,369,057 to 1,370,406
_1213
Psyr_1214: Psyr_1214 - type III secretion protein HrpQ, at 1,370,403 to 1,371,377
_1214
Psyr_1215: Psyr_1215 - Type III secretion protein HrcV, at 1,371,411 to 1,373,498
_1215
Position (kb)
1370
1371
1372 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1369.450 kb on + strand, within Psyr_1213 at 1369.457 kb on + strand, within Psyr_1213 at 1369.457 kb on + strand, within Psyr_1213 at 1369.457 kb on + strand, within Psyr_1213 at 1369.523 kb on + strand, within Psyr_1213 at 1369.524 kb on - strand, within Psyr_1213 at 1369.524 kb on - strand, within Psyr_1213 at 1369.524 kb on - strand, within Psyr_1213 at 1370.086 kb on + strand, within Psyr_1213 at 1370.086 kb on + strand, within Psyr_1213 at 1370.086 kb on + strand, within Psyr_1213 at 1370.164 kb on - strand, within Psyr_1213 at 1370.351 kb on + strand at 1370.352 kb on - strand at 1370.493 kb on + strand at 1370.493 kb on + strand at 1370.494 kb on - strand at 1370.494 kb on - strand at 1370.494 kb on - strand at 1370.494 kb on - strand at 1370.494 kb on - strand at 1370.497 kb on + strand at 1370.497 kb on + strand at 1370.497 kb on + strand at 1370.497 kb on + strand at 1370.497 kb on + strand at 1370.498 kb on - strand at 1370.498 kb on - strand at 1370.498 kb on - strand at 1370.498 kb on - strand at 1370.498 kb on - strand at 1370.498 kb on - strand at 1370.498 kb on - strand at 1370.498 kb on - strand at 1370.620 kb on + strand, within Psyr_1214 at 1370.844 kb on - strand, within Psyr_1214 at 1371.366 kb on + strand at 1371.366 kb on + strand at 1371.510 kb on + strand at 1371.510 kb on + strand at 1371.510 kb on + strand at 1371.510 kb on + strand at 1371.511 kb on - strand at 1371.511 kb on - strand at 1371.511 kb on - strand at 1371.519 kb on + strand at 1371.520 kb on - strand at 1371.520 kb on - strand at 1371.526 kb on + strand at 1371.526 kb on + strand at 1371.526 kb on + strand at 1371.526 kb on + strand at 1371.527 kb on - strand at 1371.527 kb on - strand at 1371.548 kb on + strand at 1371.548 kb on + strand at 1371.548 kb on + strand at 1371.548 kb on + strand at 1371.548 kb on + strand at 1371.549 kb on - strand at 1371.549 kb on - strand at 1371.549 kb on - strand at 1371.549 kb on - strand at 1371.549 kb on - strand at 1371.549 kb on - strand at 1371.763 kb on + strand, within Psyr_1215 at 1371.772 kb on + strand, within Psyr_1215 at 1371.772 kb on + strand, within Psyr_1215 at 1371.773 kb on - strand, within Psyr_1215 at 1371.773 kb on - strand, within Psyr_1215 at 1371.773 kb on - strand, within Psyr_1215 at 1371.773 kb on - strand, within Psyr_1215 at 1371.802 kb on + strand, within Psyr_1215 at 1371.802 kb on + strand, within Psyr_1215 at 1371.802 kb on + strand, within Psyr_1215 at 1371.802 kb on + strand, within Psyr_1215 at 1371.802 kb on + strand, within Psyr_1215 at 1371.802 kb on + strand, within Psyr_1215 at 1371.803 kb on - strand, within Psyr_1215 at 1372.242 kb on + strand, within Psyr_1215 at 1372.243 kb on - strand, within Psyr_1215 at 1372.249 kb on + strand, within Psyr_1215 at 1372.250 kb on - strand, within Psyr_1215 at 1372.250 kb on - strand, within Psyr_1215 at 1372.250 kb on - strand, within Psyr_1215 at 1372.250 kb on - strand, within Psyr_1215 at 1372.324 kb on + strand, within Psyr_1215 at 1372.356 kb on + strand, within Psyr_1215 at 1372.356 kb on + strand, within Psyr_1215
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ferulic Acid 180 ug/ml remove 1,369,450 + Psyr_1213 0.29 +1.8 1,369,457 + Psyr_1213 0.30 +0.1 1,369,457 + Psyr_1213 0.30 +1.8 1,369,457 + Psyr_1213 0.30 +0.6 1,369,523 + Psyr_1213 0.35 +0.0 1,369,524 - Psyr_1213 0.35 -1.7 1,369,524 - Psyr_1213 0.35 +1.3 1,369,524 - Psyr_1213 0.35 -0.1 1,370,086 + Psyr_1213 0.76 -1.3 1,370,086 + Psyr_1213 0.76 -1.5 1,370,086 + Psyr_1213 0.76 +1.6 1,370,164 - Psyr_1213 0.82 +1.9 1,370,351 + +0.9 1,370,352 - -0.7 1,370,493 + -0.3 1,370,493 + -0.4 1,370,494 - +0.0 1,370,494 - +0.6 1,370,494 - +0.8 1,370,494 - -3.7 1,370,494 - +1.0 1,370,497 + -1.4 1,370,497 + -0.1 1,370,497 + -1.6 1,370,497 + +0.8 1,370,497 + +0.4 1,370,498 - -0.7 1,370,498 - -0.9 1,370,498 - -1.7 1,370,498 - +1.1 1,370,498 - -0.7 1,370,498 - -0.3 1,370,498 - +0.7 1,370,498 - -0.5 1,370,620 + Psyr_1214 0.22 -1.2 1,370,844 - Psyr_1214 0.45 -3.4 1,371,366 + +0.8 1,371,366 + +1.2 1,371,510 + -0.6 1,371,510 + -0.2 1,371,510 + +1.4 1,371,510 + -0.8 1,371,511 - -0.2 1,371,511 - -0.5 1,371,511 - -0.9 1,371,519 + +1.2 1,371,520 - -0.3 1,371,520 - +0.4 1,371,526 + +0.3 1,371,526 + -2.0 1,371,526 + +0.5 1,371,526 + +1.6 1,371,527 - -0.0 1,371,527 - -0.2 1,371,548 + -0.4 1,371,548 + -2.8 1,371,548 + -1.7 1,371,548 + -0.0 1,371,548 + +2.4 1,371,549 - -0.6 1,371,549 - -0.2 1,371,549 - +0.2 1,371,549 - +0.6 1,371,549 - -3.2 1,371,549 - +2.3 1,371,763 + Psyr_1215 0.17 +0.8 1,371,772 + Psyr_1215 0.17 -0.1 1,371,772 + Psyr_1215 0.17 -1.5 1,371,773 - Psyr_1215 0.17 -0.4 1,371,773 - Psyr_1215 0.17 +0.5 1,371,773 - Psyr_1215 0.17 +0.9 1,371,773 - Psyr_1215 0.17 -2.5 1,371,802 + Psyr_1215 0.19 +0.6 1,371,802 + Psyr_1215 0.19 +0.5 1,371,802 + Psyr_1215 0.19 -1.3 1,371,802 + Psyr_1215 0.19 -0.1 1,371,802 + Psyr_1215 0.19 +0.0 1,371,802 + Psyr_1215 0.19 +4.5 1,371,803 - Psyr_1215 0.19 -0.2 1,372,242 + Psyr_1215 0.40 +0.3 1,372,243 - Psyr_1215 0.40 -1.7 1,372,249 + Psyr_1215 0.40 -0.8 1,372,250 - Psyr_1215 0.40 +0.5 1,372,250 - Psyr_1215 0.40 +0.7 1,372,250 - Psyr_1215 0.40 -0.1 1,372,250 - Psyr_1215 0.40 -0.7 1,372,324 + Psyr_1215 0.44 +0.8 1,372,356 + Psyr_1215 0.45 -3.2 1,372,356 + Psyr_1215 0.45 -0.0
Or see this region's nucleotide sequence