Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21925

Experiment: RCH2_defined_glucose with 2,2'-Dipyridyl 200 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21915 and BWI76_RS21920 are separated by 6 nucleotidesBWI76_RS21920 and BWI76_RS21925 are separated by 19 nucleotidesBWI76_RS21925 and BWI76_RS21930 are separated by 134 nucleotides BWI76_RS21915: BWI76_RS21915 - short-chain dehydrogenase/reductase SDR, at 4,579,267 to 4,580,061 _RS21915 BWI76_RS21920: BWI76_RS21920 - amino acid ABC transporter substrate-binding protein, at 4,580,068 to 4,580,284 _RS21920 BWI76_RS21925: BWI76_RS21925 - agmatinase, at 4,580,304 to 4,581,275 _RS21925 BWI76_RS21930: BWI76_RS21930 - LysR family transcriptional regulator, at 4,581,410 to 4,582,312 _RS21930 Position (kb) 4580 4581 4582Strain fitness (log2 ratio) -1 0 1at 4579.405 kb on + strand, within BWI76_RS21915at 4579.405 kb on + strand, within BWI76_RS21915at 4579.660 kb on + strand, within BWI76_RS21915at 4579.683 kb on - strand, within BWI76_RS21915at 4579.991 kb on + strandat 4580.000 kb on + strandat 4580.073 kb on + strandat 4580.143 kb on - strand, within BWI76_RS21920at 4580.145 kb on - strand, within BWI76_RS21920at 4580.152 kb on + strand, within BWI76_RS21920at 4580.152 kb on + strand, within BWI76_RS21920at 4580.595 kb on + strand, within BWI76_RS21925at 4580.630 kb on + strand, within BWI76_RS21925at 4580.673 kb on + strand, within BWI76_RS21925at 4580.673 kb on - strand, within BWI76_RS21925at 4580.998 kb on + strand, within BWI76_RS21925at 4581.154 kb on + strand, within BWI76_RS21925at 4581.159 kb on + strand, within BWI76_RS21925at 4581.167 kb on - strand, within BWI76_RS21925at 4581.284 kb on + strandat 4581.284 kb on + strandat 4581.284 kb on + strandat 4581.292 kb on - strandat 4581.404 kb on - strandat 4581.483 kb on - strandat 4581.506 kb on - strand, within BWI76_RS21930at 4581.723 kb on + strand, within BWI76_RS21930at 4581.811 kb on + strandat 4581.947 kb on + strand, within BWI76_RS21930at 4582.053 kb on + strand, within BWI76_RS21930at 4582.101 kb on + strand, within BWI76_RS21930at 4582.148 kb on + strand, within BWI76_RS21930at 4582.175 kb on - strand, within BWI76_RS21930at 4582.253 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with 2,2'-Dipyridyl 200 uM
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4,579,405 + BWI76_RS21915 0.17 +0.3
4,579,405 + BWI76_RS21915 0.17 -0.2
4,579,660 + BWI76_RS21915 0.49 -0.4
4,579,683 - BWI76_RS21915 0.52 -0.2
4,579,991 + +1.1
4,580,000 + -0.4
4,580,073 + -0.0
4,580,143 - BWI76_RS21920 0.35 -0.1
4,580,145 - BWI76_RS21920 0.35 +0.0
4,580,152 + BWI76_RS21920 0.39 -0.1
4,580,152 + BWI76_RS21920 0.39 -0.1
4,580,595 + BWI76_RS21925 0.30 +0.0
4,580,630 + BWI76_RS21925 0.34 +0.3
4,580,673 + BWI76_RS21925 0.38 -0.4
4,580,673 - BWI76_RS21925 0.38 +1.1
4,580,998 + BWI76_RS21925 0.71 +0.7
4,581,154 + BWI76_RS21925 0.87 +0.8
4,581,159 + BWI76_RS21925 0.88 -1.7
4,581,167 - BWI76_RS21925 0.89 -0.1
4,581,284 + -0.2
4,581,284 + +0.7
4,581,284 + +0.4
4,581,292 - -0.2
4,581,404 - +1.0
4,581,483 - +0.2
4,581,506 - BWI76_RS21930 0.11 -0.6
4,581,723 + BWI76_RS21930 0.35 +1.1
4,581,811 + -0.2
4,581,947 + BWI76_RS21930 0.59 +0.1
4,582,053 + BWI76_RS21930 0.71 -0.3
4,582,101 + BWI76_RS21930 0.77 +0.1
4,582,148 + BWI76_RS21930 0.82 -0.2
4,582,175 - BWI76_RS21930 0.85 -0.9
4,582,253 + +0.5

Or see this region's nucleotide sequence