Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS22035 and HSERO_RS22040 are separated by 425 nucleotides HSERO_RS22040 and HSERO_RS22045 are separated by 54 nucleotides
HSERO_RS22035: HSERO_RS22035 - tRNA 2-thiocytidine biosynthesis protein TtcA, at 5,037,786 to 5,038,655
_RS22035
HSERO_RS22040: HSERO_RS22040 - CDP-glucose 4,6-dehydratase, at 5,039,081 to 5,040,157
_RS22040
HSERO_RS22045: HSERO_RS22045 - UDP-glucose 4-epimerase, at 5,040,212 to 5,041,222
_RS22045
Position (kb)
5039
5040
5041 Strain fitness (log2 ratio)
-1
0
1
2 at 5038.118 kb on + strand, within HSERO_RS22035 at 5038.129 kb on + strand, within HSERO_RS22035 at 5038.149 kb on - strand, within HSERO_RS22035 at 5038.237 kb on + strand, within HSERO_RS22035 at 5038.268 kb on + strand, within HSERO_RS22035 at 5038.276 kb on - strand, within HSERO_RS22035 at 5038.401 kb on - strand, within HSERO_RS22035 at 5038.423 kb on - strand, within HSERO_RS22035 at 5038.564 kb on + strand at 5038.726 kb on + strand at 5038.866 kb on + strand at 5038.998 kb on + strand at 5039.143 kb on + strand at 5039.975 kb on - strand, within HSERO_RS22040 at 5040.003 kb on + strand, within HSERO_RS22040 at 5040.011 kb on - strand, within HSERO_RS22040 at 5040.046 kb on + strand at 5040.126 kb on - strand at 5040.135 kb on + strand at 5040.194 kb on + strand at 5040.271 kb on + strand at 5040.271 kb on + strand at 5040.279 kb on - strand at 5040.373 kb on + strand, within HSERO_RS22045 at 5040.411 kb on - strand, within HSERO_RS22045 at 5040.457 kb on + strand, within HSERO_RS22045 at 5040.465 kb on - strand, within HSERO_RS22045 at 5040.589 kb on + strand, within HSERO_RS22045
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 5,038,118 + HSERO_RS22035 0.38 -0.3 5,038,129 + HSERO_RS22035 0.39 +0.2 5,038,149 - HSERO_RS22035 0.42 +0.4 5,038,237 + HSERO_RS22035 0.52 -0.1 5,038,268 + HSERO_RS22035 0.55 +0.2 5,038,276 - HSERO_RS22035 0.56 +0.3 5,038,401 - HSERO_RS22035 0.71 +0.1 5,038,423 - HSERO_RS22035 0.73 -0.3 5,038,564 + +0.7 5,038,726 + -0.1 5,038,866 + -0.1 5,038,998 + -0.5 5,039,143 + +0.0 5,039,975 - HSERO_RS22040 0.83 +0.2 5,040,003 + HSERO_RS22040 0.86 -1.4 5,040,011 - HSERO_RS22040 0.86 -0.9 5,040,046 + +1.0 5,040,126 - -0.2 5,040,135 + +1.8 5,040,194 + +0.1 5,040,271 + +0.3 5,040,271 + +0.8 5,040,279 - -0.4 5,040,373 + HSERO_RS22045 0.16 -0.0 5,040,411 - HSERO_RS22045 0.20 +0.4 5,040,457 + HSERO_RS22045 0.24 -0.9 5,040,465 - HSERO_RS22045 0.25 -0.4 5,040,589 + HSERO_RS22045 0.37 +2.3
Or see this region's nucleotide sequence