Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS20120 and HSERO_RS20125 are separated by 225 nucleotides HSERO_RS20125 and HSERO_RS20130 are separated by 98 nucleotides
HSERO_RS20120: HSERO_RS20120 - peptidase M15B and M15C, D,D-carboxypeptidase VanY/endolysins protein, at 4,617,749 to 4,618,588
_RS20120
HSERO_RS20125: HSERO_RS20125 - hypothetical protein, at 4,618,814 to 4,619,245
_RS20125
HSERO_RS20130: HSERO_RS20130 - type VI secretion protein VasK, at 4,619,344 to 4,623,144
_RS20130
Position (kb) 4618
4619
4620 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4617.948 kb on + strand, within HSERO_RS20120 at 4617.948 kb on + strand, within HSERO_RS20120 at 4617.948 kb on + strand, within HSERO_RS20120 at 4617.948 kb on + strand, within HSERO_RS20120 at 4617.953 kb on + strand, within HSERO_RS20120 at 4617.953 kb on + strand, within HSERO_RS20120 at 4617.956 kb on - strand, within HSERO_RS20120 at 4618.001 kb on + strand, within HSERO_RS20120 at 4618.062 kb on + strand, within HSERO_RS20120 at 4618.375 kb on + strand, within HSERO_RS20120 at 4618.553 kb on + strand at 4618.745 kb on - strand at 4618.750 kb on - strand at 4618.765 kb on + strand at 4618.765 kb on + strand at 4618.766 kb on + strand at 4618.766 kb on + strand at 4618.774 kb on - strand at 4618.774 kb on - strand at 4618.846 kb on - strand at 4618.866 kb on + strand, within HSERO_RS20125 at 4619.026 kb on + strand, within HSERO_RS20125 at 4619.034 kb on - strand, within HSERO_RS20125 at 4619.193 kb on - strand at 4619.203 kb on + strand at 4619.222 kb on + strand at 4619.391 kb on + strand at 4619.489 kb on - strand at 4619.627 kb on + strand at 4619.650 kb on + strand at 4619.782 kb on + strand, within HSERO_RS20130 at 4620.131 kb on - strand, within HSERO_RS20130
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 4,617,948 + HSERO_RS20120 0.24 +0.3 4,617,948 + HSERO_RS20120 0.24 -0.0 4,617,948 + HSERO_RS20120 0.24 -0.5 4,617,948 + HSERO_RS20120 0.24 +0.3 4,617,953 + HSERO_RS20120 0.24 -1.9 4,617,953 + HSERO_RS20120 0.24 +0.2 4,617,956 - HSERO_RS20120 0.25 -0.2 4,618,001 + HSERO_RS20120 0.30 +0.2 4,618,062 + HSERO_RS20120 0.37 +0.2 4,618,375 + HSERO_RS20120 0.75 +0.1 4,618,553 + +0.1 4,618,745 - +0.4 4,618,750 - -0.4 4,618,765 + -0.4 4,618,765 + -0.1 4,618,766 + -0.4 4,618,766 + -0.0 4,618,774 - +0.0 4,618,774 - +0.7 4,618,846 - +0.4 4,618,866 + HSERO_RS20125 0.12 -0.7 4,619,026 + HSERO_RS20125 0.49 -0.9 4,619,034 - HSERO_RS20125 0.51 -0.3 4,619,193 - +2.2 4,619,203 + +0.5 4,619,222 + -0.1 4,619,391 + +0.1 4,619,489 - +0.9 4,619,627 + -0.4 4,619,650 + +0.1 4,619,782 + HSERO_RS20130 0.12 +0.3 4,620,131 - HSERO_RS20130 0.21 -0.0
Or see this region's nucleotide sequence