Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS18505 and HSERO_RS18510 overlap by 8 nucleotides HSERO_RS18510 and HSERO_RS18515 overlap by 4 nucleotides HSERO_RS18515 and HSERO_RS18520 overlap by 8 nucleotides
HSERO_RS18505: HSERO_RS18505 - histidine ammonia-lyase, at 4,246,369 to 4,247,949
_RS18505
HSERO_RS18510: HSERO_RS18510 - thioesterase, at 4,247,942 to 4,248,406
_RS18510
HSERO_RS18515: HSERO_RS18515 - membrane protein, at 4,248,403 to 4,249,014
_RS18515
HSERO_RS18520: HSERO_RS18520 - exporter, at 4,249,007 to 4,251,439
_RS18520
Position (kb) 4248
4249
4250 Strain fitness (log2 ratio) -1
0
1 at 4247.485 kb on + strand, within HSERO_RS18505 at 4247.560 kb on - strand, within HSERO_RS18505 at 4247.590 kb on + strand, within HSERO_RS18505 at 4247.597 kb on + strand, within HSERO_RS18505 at 4247.663 kb on - strand, within HSERO_RS18505 at 4247.675 kb on + strand, within HSERO_RS18505 at 4247.721 kb on + strand, within HSERO_RS18505 at 4247.756 kb on + strand, within HSERO_RS18505 at 4247.855 kb on - strand at 4247.928 kb on + strand at 4247.983 kb on - strand at 4248.024 kb on - strand, within HSERO_RS18510 at 4248.129 kb on + strand, within HSERO_RS18510 at 4248.165 kb on + strand, within HSERO_RS18510 at 4248.508 kb on + strand, within HSERO_RS18515 at 4248.738 kb on - strand, within HSERO_RS18515 at 4249.057 kb on + strand at 4249.115 kb on - strand at 4249.440 kb on + strand, within HSERO_RS18520 at 4249.536 kb on + strand, within HSERO_RS18520 at 4249.546 kb on - strand, within HSERO_RS18520 at 4249.557 kb on - strand, within HSERO_RS18520 at 4249.612 kb on - strand, within HSERO_RS18520 at 4249.612 kb on - strand, within HSERO_RS18520 at 4249.745 kb on + strand, within HSERO_RS18520 at 4249.852 kb on + strand, within HSERO_RS18520 at 4249.987 kb on + strand, within HSERO_RS18520
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 4,247,485 + HSERO_RS18505 0.71 -0.2 4,247,560 - HSERO_RS18505 0.75 +0.1 4,247,590 + HSERO_RS18505 0.77 +0.4 4,247,597 + HSERO_RS18505 0.78 -0.5 4,247,663 - HSERO_RS18505 0.82 -0.3 4,247,675 + HSERO_RS18505 0.83 +0.5 4,247,721 + HSERO_RS18505 0.86 +0.2 4,247,756 + HSERO_RS18505 0.88 +1.7 4,247,855 - +1.1 4,247,928 + -0.9 4,247,983 - +0.0 4,248,024 - HSERO_RS18510 0.18 +1.1 4,248,129 + HSERO_RS18510 0.40 +0.1 4,248,165 + HSERO_RS18510 0.48 -0.2 4,248,508 + HSERO_RS18515 0.17 -0.5 4,248,738 - HSERO_RS18515 0.55 -0.6 4,249,057 + -1.5 4,249,115 - -0.4 4,249,440 + HSERO_RS18520 0.18 +0.2 4,249,536 + HSERO_RS18520 0.22 -1.0 4,249,546 - HSERO_RS18520 0.22 -0.3 4,249,557 - HSERO_RS18520 0.23 -1.0 4,249,612 - HSERO_RS18520 0.25 +0.1 4,249,612 - HSERO_RS18520 0.25 -0.3 4,249,745 + HSERO_RS18520 0.30 +0.5 4,249,852 + HSERO_RS18520 0.35 +0.7 4,249,987 + HSERO_RS18520 0.40 -1.6
Or see this region's nucleotide sequence