Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS13645 and HSERO_RS13650 are separated by 58 nucleotides HSERO_RS13650 and HSERO_RS13655 are separated by 45 nucleotides HSERO_RS13655 and HSERO_RS13660 are separated by 127 nucleotides
HSERO_RS13645: HSERO_RS13645 - membrane protein, at 3,098,376 to 3,099,713
_RS13645
HSERO_RS13650: HSERO_RS13650 - high-affinity Fe2+/Pb2+ permease, at 3,099,772 to 3,100,644
_RS13650
HSERO_RS13655: HSERO_RS13655 - hypothetical protein, at 3,100,690 to 3,101,031
_RS13655
HSERO_RS13660: HSERO_RS13660 - membrane protein, at 3,101,159 to 3,101,704
_RS13660
Position (kb)
3099
3100
3101 Strain fitness (log2 ratio)
-1
0
1 at 3098.991 kb on + strand at 3099.222 kb on - strand, within HSERO_RS13645 at 3099.284 kb on + strand, within HSERO_RS13645 at 3099.505 kb on + strand, within HSERO_RS13645 at 3099.592 kb on - strand at 3099.826 kb on + strand at 3099.844 kb on - strand at 3099.844 kb on - strand at 3099.918 kb on + strand, within HSERO_RS13650 at 3100.158 kb on + strand, within HSERO_RS13650 at 3100.212 kb on + strand, within HSERO_RS13650 at 3100.317 kb on - strand, within HSERO_RS13650 at 3100.323 kb on + strand, within HSERO_RS13650 at 3100.453 kb on - strand, within HSERO_RS13650 at 3100.473 kb on - strand, within HSERO_RS13650 at 3100.956 kb on - strand, within HSERO_RS13655 at 3100.981 kb on + strand, within HSERO_RS13655 at 3101.533 kb on - strand, within HSERO_RS13660
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 3,098,991 + -1.0 3,099,222 - HSERO_RS13645 0.63 -0.4 3,099,284 + HSERO_RS13645 0.68 +0.2 3,099,505 + HSERO_RS13645 0.84 +0.4 3,099,592 - -0.5 3,099,826 + +1.0 3,099,844 - -0.4 3,099,844 - -0.6 3,099,918 + HSERO_RS13650 0.17 -0.1 3,100,158 + HSERO_RS13650 0.44 -0.7 3,100,212 + HSERO_RS13650 0.50 -0.2 3,100,317 - HSERO_RS13650 0.62 -0.9 3,100,323 + HSERO_RS13650 0.63 -0.2 3,100,453 - HSERO_RS13650 0.78 -0.1 3,100,473 - HSERO_RS13650 0.80 -1.4 3,100,956 - HSERO_RS13655 0.78 -0.4 3,100,981 + HSERO_RS13655 0.85 -0.5 3,101,533 - HSERO_RS13660 0.68 -0.3
Or see this region's nucleotide sequence