Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS13480 and HSERO_RS13485 are separated by 146 nucleotides HSERO_RS13485 and HSERO_RS13490 are separated by 11 nucleotides HSERO_RS13490 and HSERO_RS13495 overlap by 4 nucleotides
HSERO_RS13480: HSERO_RS13480 - C4-dicarboxylate ABC transporter, at 3,065,120 to 3,066,142
_RS13480
HSERO_RS13485: HSERO_RS13485 - AsnC family transcriptional regulator, at 3,066,289 to 3,066,957
_RS13485
HSERO_RS13490: HSERO_RS13490 - pseudouridine synthase, at 3,066,969 to 3,067,868
_RS13490
HSERO_RS13495: HSERO_RS13495 - phosphatidylinositol kinase, at 3,067,865 to 3,069,205
_RS13495
Position (kb)
3066
3067
3068 Strain fitness (log2 ratio)
-1
0
1 at 3066.190 kb on + strand at 3066.218 kb on + strand at 3066.234 kb on + strand at 3066.280 kb on - strand at 3066.280 kb on - strand at 3066.294 kb on - strand at 3066.797 kb on + strand, within HSERO_RS13485 at 3066.806 kb on - strand at 3066.901 kb on + strand at 3067.220 kb on + strand, within HSERO_RS13490 at 3067.381 kb on - strand, within HSERO_RS13490 at 3067.673 kb on - strand, within HSERO_RS13490 at 3067.779 kb on + strand at 3068.332 kb on - strand, within HSERO_RS13495 at 3068.555 kb on + strand, within HSERO_RS13495 at 3068.665 kb on + strand, within HSERO_RS13495
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 3,066,190 + -0.3 3,066,218 + -0.7 3,066,234 + -0.2 3,066,280 - -0.3 3,066,280 - +1.3 3,066,294 - -0.3 3,066,797 + HSERO_RS13485 0.76 -0.1 3,066,806 - +0.6 3,066,901 + -0.2 3,067,220 + HSERO_RS13490 0.28 -0.1 3,067,381 - HSERO_RS13490 0.46 -1.2 3,067,673 - HSERO_RS13490 0.78 -1.0 3,067,779 + -0.1 3,068,332 - HSERO_RS13495 0.35 -0.1 3,068,555 + HSERO_RS13495 0.51 -0.4 3,068,665 + HSERO_RS13495 0.60 -0.4
Or see this region's nucleotide sequence