Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS12515 and HSERO_RS12520 are separated by 564 nucleotides HSERO_RS12520 and HSERO_RS12525 are separated by 402 nucleotides HSERO_RS12525 and HSERO_RS12530 are separated by 128 nucleotides
HSERO_RS12515: HSERO_RS12515 - glycogen synthase, at 2,854,929 to 2,856,518
_RS12515
HSERO_RS12520: HSERO_RS12520 - hypothetical protein, at 2,857,083 to 2,857,310
_RS12520
HSERO_RS12525: HSERO_RS12525 - hypothetical protein, at 2,857,713 to 2,857,895
_RS12525
HSERO_RS12530: HSERO_RS12530 - transcriptional regulator, at 2,858,024 to 2,859,604
_RS12530
Position (kb)
2857
2858 Strain fitness (log2 ratio)
-1
0
1 at 2856.466 kb on + strand at 2856.503 kb on - strand at 2856.777 kb on - strand at 2856.883 kb on - strand at 2856.907 kb on + strand at 2856.907 kb on + strand at 2856.909 kb on + strand at 2856.952 kb on - strand at 2857.003 kb on - strand at 2857.003 kb on - strand at 2857.010 kb on + strand at 2857.169 kb on + strand, within HSERO_RS12520 at 2857.209 kb on + strand, within HSERO_RS12520 at 2857.262 kb on - strand, within HSERO_RS12520 at 2857.475 kb on - strand at 2857.475 kb on - strand at 2857.658 kb on + strand at 2857.754 kb on + strand, within HSERO_RS12525 at 2857.762 kb on - strand, within HSERO_RS12525 at 2858.022 kb on + strand at 2858.209 kb on + strand, within HSERO_RS12530 at 2858.255 kb on + strand, within HSERO_RS12530
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 2,856,466 + +0.5 2,856,503 - +0.0 2,856,777 - -0.3 2,856,883 - -0.5 2,856,907 + +0.4 2,856,907 + -0.2 2,856,909 + +0.6 2,856,952 - +0.1 2,857,003 - -0.2 2,857,003 - +0.6 2,857,010 + +0.7 2,857,169 + HSERO_RS12520 0.38 -0.4 2,857,209 + HSERO_RS12520 0.55 -0.1 2,857,262 - HSERO_RS12520 0.79 -1.2 2,857,475 - +0.5 2,857,475 - +1.0 2,857,658 + +0.2 2,857,754 + HSERO_RS12525 0.22 +0.7 2,857,762 - HSERO_RS12525 0.27 -0.0 2,858,022 + +0.1 2,858,209 + HSERO_RS12530 0.12 -0.4 2,858,255 + HSERO_RS12530 0.15 +0.5
Or see this region's nucleotide sequence