Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS10940 and HSERO_RS10945 are separated by 68 nucleotides HSERO_RS10945 and HSERO_RS10950 are separated by 10 nucleotides HSERO_RS10950 and HSERO_RS10955 are separated by 25 nucleotides
HSERO_RS10940: HSERO_RS10940 - lipid-A-disaccharide synthase, at 2,467,184 to 2,468,359
_RS10940
HSERO_RS10945: HSERO_RS10945 - ribonuclease HII, at 2,468,428 to 2,469,060
_RS10945
HSERO_RS10950: HSERO_RS10950 - RNA methyltransferase, at 2,469,071 to 2,469,862
_RS10950
HSERO_RS10955: HSERO_RS10955 - hypothetical protein, at 2,469,888 to 2,470,373
_RS10955
Position (kb)
2468
2469
2470 Strain fitness (log2 ratio)
-1
0
1 at 2468.389 kb on - strand at 2468.644 kb on + strand, within HSERO_RS10945 at 2468.708 kb on + strand, within HSERO_RS10945 at 2468.762 kb on - strand, within HSERO_RS10945 at 2468.998 kb on - strand at 2469.018 kb on + strand at 2469.092 kb on - strand at 2469.155 kb on + strand, within HSERO_RS10950 at 2469.163 kb on - strand, within HSERO_RS10950 at 2469.164 kb on + strand, within HSERO_RS10950 at 2469.220 kb on + strand, within HSERO_RS10950 at 2469.228 kb on - strand, within HSERO_RS10950 at 2469.474 kb on + strand, within HSERO_RS10950 at 2469.591 kb on + strand, within HSERO_RS10950 at 2469.742 kb on - strand, within HSERO_RS10950 at 2469.880 kb on + strand at 2469.969 kb on + strand, within HSERO_RS10955
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 2,468,389 - +0.2 2,468,644 + HSERO_RS10945 0.34 +0.2 2,468,708 + HSERO_RS10945 0.44 -1.0 2,468,762 - HSERO_RS10945 0.53 -1.1 2,468,998 - -0.3 2,469,018 + +0.1 2,469,092 - +0.1 2,469,155 + HSERO_RS10950 0.11 -0.1 2,469,163 - HSERO_RS10950 0.12 +0.2 2,469,164 + HSERO_RS10950 0.12 -0.2 2,469,220 + HSERO_RS10950 0.19 +0.2 2,469,228 - HSERO_RS10950 0.20 +0.6 2,469,474 + HSERO_RS10950 0.51 -0.5 2,469,591 + HSERO_RS10950 0.66 +0.1 2,469,742 - HSERO_RS10950 0.85 -0.7 2,469,880 + -0.0 2,469,969 + HSERO_RS10955 0.17 -0.1
Or see this region's nucleotide sequence