Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS04315 and HSERO_RS04320 are separated by 131 nucleotides HSERO_RS04320 and HSERO_RS04325 are separated by 446 nucleotides HSERO_RS04325 and HSERO_RS04330 overlap by 25 nucleotides
HSERO_RS04315: HSERO_RS04315 - transcription termination factor Rho, at 949,021 to 950,283
_RS04315
HSERO_RS04320: HSERO_RS04320 - thioredoxin, at 950,415 to 950,741
_RS04320
HSERO_RS04325: HSERO_RS04325 - helicase I, at 951,188 to 953,962
_RS04325
HSERO_RS04330: HSERO_RS04330 - ATP-dependent exonuclease, at 953,938 to 957,435
_RS04330
Position (kb) 951
952
953
954 Strain fitness (log2 ratio) -2
-1
0
1
2 at 950.915 kb on + strand at 951.084 kb on - strand at 951.103 kb on - strand at 951.379 kb on - strand at 951.488 kb on + strand, within HSERO_RS04325 at 951.560 kb on + strand, within HSERO_RS04325 at 951.849 kb on - strand, within HSERO_RS04325 at 951.932 kb on + strand, within HSERO_RS04325 at 951.940 kb on - strand at 952.093 kb on - strand, within HSERO_RS04325 at 952.637 kb on + strand, within HSERO_RS04325 at 952.683 kb on + strand, within HSERO_RS04325 at 952.729 kb on - strand, within HSERO_RS04325 at 952.766 kb on + strand, within HSERO_RS04325 at 952.837 kb on - strand, within HSERO_RS04325 at 952.911 kb on + strand, within HSERO_RS04325 at 952.980 kb on - strand, within HSERO_RS04325 at 953.221 kb on - strand, within HSERO_RS04325 at 953.941 kb on + strand at 954.317 kb on + strand, within HSERO_RS04330 at 954.346 kb on - strand, within HSERO_RS04330 at 954.885 kb on - strand, within HSERO_RS04330 at 954.947 kb on + strand, within HSERO_RS04330
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 950,915 + -1.0 951,084 - -0.3 951,103 - +0.9 951,379 - -1.1 951,488 + HSERO_RS04325 0.11 +0.6 951,560 + HSERO_RS04325 0.13 -0.2 951,849 - HSERO_RS04325 0.24 -1.9 951,932 + HSERO_RS04325 0.27 -2.0 951,940 - -0.4 952,093 - HSERO_RS04325 0.33 -0.9 952,637 + HSERO_RS04325 0.52 -1.5 952,683 + HSERO_RS04325 0.54 -1.8 952,729 - HSERO_RS04325 0.56 -0.7 952,766 + HSERO_RS04325 0.57 +1.4 952,837 - HSERO_RS04325 0.59 -1.0 952,911 + HSERO_RS04325 0.62 -0.3 952,980 - HSERO_RS04325 0.65 -0.1 953,221 - HSERO_RS04325 0.73 -0.4 953,941 + -0.4 954,317 + HSERO_RS04330 0.11 +2.5 954,346 - HSERO_RS04330 0.12 -0.2 954,885 - HSERO_RS04330 0.27 -0.8 954,947 + HSERO_RS04330 0.29 -1.1
Or see this region's nucleotide sequence