Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS03315 and HSERO_RS03320 are separated by 56 nucleotides HSERO_RS03320 and HSERO_RS03325 are separated by 66 nucleotides HSERO_RS03325 and HSERO_RS03330 are separated by 97 nucleotides
HSERO_RS03315: HSERO_RS03315 - alpha/beta hydrolase, at 729,023 to 730,015
_RS03315
HSERO_RS03320: HSERO_RS03320 - ketosteroid isomerase, at 730,072 to 730,503
_RS03320
HSERO_RS03325: HSERO_RS03325 - hypothetical protein, at 730,570 to 730,770
_RS03325
HSERO_RS03330: HSERO_RS03330 - branched-chain amino acid aminotransferase, at 730,868 to 731,788
_RS03330
Position (kb) 730
731 Strain fitness (log2 ratio) -1
0
1 at 729.645 kb on - strand, within HSERO_RS03315 at 729.942 kb on - strand at 729.969 kb on + strand at 730.012 kb on + strand at 730.030 kb on - strand at 730.148 kb on - strand, within HSERO_RS03320 at 730.192 kb on + strand, within HSERO_RS03320 at 730.350 kb on + strand, within HSERO_RS03320 at 730.352 kb on + strand, within HSERO_RS03320 at 730.402 kb on - strand, within HSERO_RS03320 at 730.478 kb on + strand at 730.495 kb on - strand at 730.523 kb on + strand at 730.531 kb on - strand at 730.745 kb on - strand, within HSERO_RS03325 at 730.791 kb on - strand at 730.841 kb on + strand at 730.850 kb on - strand at 730.850 kb on - strand at 730.859 kb on - strand at 730.866 kb on + strand at 730.866 kb on + strand at 730.874 kb on - strand at 730.874 kb on - strand at 730.874 kb on - strand at 730.876 kb on + strand at 731.160 kb on + strand, within HSERO_RS03330 at 731.168 kb on - strand, within HSERO_RS03330 at 731.266 kb on - strand, within HSERO_RS03330 at 731.478 kb on + strand, within HSERO_RS03330 at 731.688 kb on + strand, within HSERO_RS03330 at 731.688 kb on - strand, within HSERO_RS03330 at 731.696 kb on - strand, within HSERO_RS03330 at 731.713 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 729,645 - HSERO_RS03315 0.63 +0.1 729,942 - -0.1 729,969 + -0.1 730,012 + -0.1 730,030 - -0.7 730,148 - HSERO_RS03320 0.18 -0.7 730,192 + HSERO_RS03320 0.28 +0.1 730,350 + HSERO_RS03320 0.64 +0.2 730,352 + HSERO_RS03320 0.65 +0.6 730,402 - HSERO_RS03320 0.76 -0.2 730,478 + -0.6 730,495 - +0.4 730,523 + +0.3 730,531 - -1.0 730,745 - HSERO_RS03325 0.87 -0.6 730,791 - -0.1 730,841 + -1.6 730,850 - +0.4 730,850 - -0.1 730,859 - +1.3 730,866 + -1.6 730,866 + -0.6 730,874 - +1.0 730,874 - -0.2 730,874 - -0.2 730,876 + +0.0 731,160 + HSERO_RS03330 0.32 -0.2 731,168 - HSERO_RS03330 0.33 -0.5 731,266 - HSERO_RS03330 0.43 -0.3 731,478 + HSERO_RS03330 0.66 +0.1 731,688 + HSERO_RS03330 0.89 +0.0 731,688 - HSERO_RS03330 0.89 +0.3 731,696 - HSERO_RS03330 0.90 -0.0 731,713 - +0.7
Or see this region's nucleotide sequence