Experiment: RCH2_defined_glucose with 2,2'-Dipyridyl 100 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23335 and BWI76_RS23340 overlap by 4 nucleotides BWI76_RS23340 and BWI76_RS23345 are separated by 68 nucleotides BWI76_RS23345 and BWI76_RS23350 are separated by 7 nucleotides
BWI76_RS23335: BWI76_RS23335 - GNAT family acetyltransferase, at 4,865,817 to 4,866,296
_RS23335
BWI76_RS23340: BWI76_RS23340 - transcriptional regulator, at 4,866,293 to 4,867,066
_RS23340
BWI76_RS23345: BWI76_RS23345 - glycoside hydrolase, at 4,867,135 to 4,868,562
_RS23345
BWI76_RS23350: BWI76_RS23350 - PTS system lactose/cellobiose family transporter subunit IIC, at 4,868,570 to 4,869,907
_RS23350
Position (kb) 4867
4868
4869 Strain fitness (log2 ratio) -1
0
1
2 at 4866.161 kb on + strand, within BWI76_RS23335 at 4866.251 kb on + strand at 4866.274 kb on + strand at 4866.283 kb on + strand at 4866.296 kb on + strand at 4866.597 kb on + strand, within BWI76_RS23340 at 4866.858 kb on - strand, within BWI76_RS23340 at 4867.718 kb on + strand, within BWI76_RS23345 at 4868.058 kb on + strand, within BWI76_RS23345 at 4868.198 kb on - strand, within BWI76_RS23345 at 4868.198 kb on - strand, within BWI76_RS23345 at 4868.252 kb on - strand, within BWI76_RS23345 at 4868.511 kb on + strand at 4868.906 kb on + strand, within BWI76_RS23350 at 4868.914 kb on - strand, within BWI76_RS23350 at 4868.966 kb on + strand, within BWI76_RS23350 at 4869.070 kb on + strand, within BWI76_RS23350 at 4869.078 kb on - strand, within BWI76_RS23350 at 4869.126 kb on + strand, within BWI76_RS23350 at 4869.167 kb on - strand at 4869.206 kb on + strand, within BWI76_RS23350 at 4869.242 kb on + strand, within BWI76_RS23350 at 4869.250 kb on - strand, within BWI76_RS23350 at 4869.262 kb on + strand, within BWI76_RS23350
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with 2,2'-Dipyridyl 100 uM remove 4,866,161 + BWI76_RS23335 0.72 -0.4 4,866,251 + -0.1 4,866,274 + -0.6 4,866,283 + +0.2 4,866,296 + -0.0 4,866,597 + BWI76_RS23340 0.39 +0.2 4,866,858 - BWI76_RS23340 0.73 -0.4 4,867,718 + BWI76_RS23345 0.41 +0.2 4,868,058 + BWI76_RS23345 0.65 -0.1 4,868,198 - BWI76_RS23345 0.74 +0.7 4,868,198 - BWI76_RS23345 0.74 +0.0 4,868,252 - BWI76_RS23345 0.78 +0.6 4,868,511 + +0.1 4,868,906 + BWI76_RS23350 0.25 +0.6 4,868,914 - BWI76_RS23350 0.26 +2.0 4,868,966 + BWI76_RS23350 0.30 -0.3 4,869,070 + BWI76_RS23350 0.37 -0.2 4,869,078 - BWI76_RS23350 0.38 -0.4 4,869,126 + BWI76_RS23350 0.42 -0.7 4,869,167 - +0.0 4,869,206 + BWI76_RS23350 0.48 +0.5 4,869,242 + BWI76_RS23350 0.50 -0.2 4,869,250 - BWI76_RS23350 0.51 -0.0 4,869,262 + BWI76_RS23350 0.52 -0.7
Or see this region's nucleotide sequence