Experiment: RCH2_defined_glucose with 2,2'-Dipyridyl 100 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09530 and BWI76_RS09535 overlap by 4 nucleotides BWI76_RS09535 and BWI76_RS09540 overlap by 4 nucleotides BWI76_RS09540 and BWI76_RS09545 are separated by 96 nucleotides
BWI76_RS09530: BWI76_RS09530 - hypothetical protein, at 1,993,567 to 1,993,890
_RS09530
BWI76_RS09535: BWI76_RS09535 - N-acetylmuramoyl-L-alanine amidase amid, at 1,993,887 to 1,994,717
_RS09535
BWI76_RS09540: BWI76_RS09540 - hypothetical protein, at 1,994,714 to 1,995,727
_RS09540
BWI76_RS09545: BWI76_RS09545 - NAD-dependent epimerase/dehydratase, at 1,995,824 to 1,997,257
_RS09545
Position (kb)
1994
1995
1996 Strain fitness (log2 ratio)
-1
0
1 at 1993.846 kb on + strand, within BWI76_RS09530 at 1993.990 kb on + strand, within BWI76_RS09535 at 1994.103 kb on - strand, within BWI76_RS09535 at 1994.244 kb on - strand, within BWI76_RS09535 at 1994.512 kb on + strand, within BWI76_RS09535 at 1994.582 kb on + strand, within BWI76_RS09535 at 1994.736 kb on + strand at 1994.934 kb on + strand, within BWI76_RS09540 at 1995.367 kb on + strand, within BWI76_RS09540 at 1995.592 kb on - strand, within BWI76_RS09540 at 1995.999 kb on - strand, within BWI76_RS09545 at 1995.999 kb on - strand, within BWI76_RS09545 at 1996.322 kb on - strand, within BWI76_RS09545 at 1996.669 kb on - strand, within BWI76_RS09545
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with 2,2'-Dipyridyl 100 uM remove 1,993,846 + BWI76_RS09530 0.86 -0.6 1,993,990 + BWI76_RS09535 0.12 +0.5 1,994,103 - BWI76_RS09535 0.26 -0.0 1,994,244 - BWI76_RS09535 0.43 -0.1 1,994,512 + BWI76_RS09535 0.75 -1.4 1,994,582 + BWI76_RS09535 0.84 -0.1 1,994,736 + +0.5 1,994,934 + BWI76_RS09540 0.22 +1.4 1,995,367 + BWI76_RS09540 0.64 +0.2 1,995,592 - BWI76_RS09540 0.87 +0.2 1,995,999 - BWI76_RS09545 0.12 -0.1 1,995,999 - BWI76_RS09545 0.12 -0.2 1,996,322 - BWI76_RS09545 0.35 -0.0 1,996,669 - BWI76_RS09545 0.59 -0.1
Or see this region's nucleotide sequence