Experiment: RCH2_defined_glucose with 2,2'-Dipyridyl 100 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04600 and BWI76_RS04605 overlap by 14 nucleotides BWI76_RS04605 and BWI76_RS04610 overlap by 17 nucleotides BWI76_RS04610 and BWI76_RS04615 overlap by 25 nucleotides
BWI76_RS04600: BWI76_RS04600 - inner membrane protein, at 957,752 to 958,519
_RS04600
BWI76_RS04605: BWI76_RS04605 - thiamine ABC transporter ATP-binding protein, at 958,506 to 959,207
_RS04605
BWI76_RS04610: BWI76_RS04610 - thiamine/thiamine pyrophosphate ABC transporter permease ThiP, at 959,191 to 960,801
_RS04610
BWI76_RS04615: BWI76_RS04615 - thiamine transporter substrate binding subunit, at 960,777 to 961,760
_RS04615
Position (kb) 959
960
961 Strain fitness (log2 ratio) -1
0
1
2
3 at 958.400 kb on + strand, within BWI76_RS04600 at 958.852 kb on + strand, within BWI76_RS04605 at 959.409 kb on - strand, within BWI76_RS04610 at 959.490 kb on + strand, within BWI76_RS04610 at 959.622 kb on - strand, within BWI76_RS04610 at 959.760 kb on + strand, within BWI76_RS04610 at 959.799 kb on + strand, within BWI76_RS04610 at 959.944 kb on + strand, within BWI76_RS04610 at 959.944 kb on + strand, within BWI76_RS04610 at 960.041 kb on - strand at 960.227 kb on + strand, within BWI76_RS04610 at 960.236 kb on - strand, within BWI76_RS04610 at 960.254 kb on - strand, within BWI76_RS04610 at 960.445 kb on + strand, within BWI76_RS04610 at 960.448 kb on - strand, within BWI76_RS04610 at 960.506 kb on - strand, within BWI76_RS04610 at 960.555 kb on + strand, within BWI76_RS04610 at 960.909 kb on - strand, within BWI76_RS04615 at 961.024 kb on + strand, within BWI76_RS04615 at 961.132 kb on - strand, within BWI76_RS04615 at 961.132 kb on - strand, within BWI76_RS04615 at 961.321 kb on + strand, within BWI76_RS04615 at 961.329 kb on - strand, within BWI76_RS04615 at 961.495 kb on + strand, within BWI76_RS04615 at 961.674 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with 2,2'-Dipyridyl 100 uM remove 958,400 + BWI76_RS04600 0.84 +0.2 958,852 + BWI76_RS04605 0.49 -0.1 959,409 - BWI76_RS04610 0.14 +0.2 959,490 + BWI76_RS04610 0.19 +0.5 959,622 - BWI76_RS04610 0.27 -0.1 959,760 + BWI76_RS04610 0.35 +0.0 959,799 + BWI76_RS04610 0.38 +1.3 959,944 + BWI76_RS04610 0.47 +2.0 959,944 + BWI76_RS04610 0.47 -0.1 960,041 - +3.6 960,227 + BWI76_RS04610 0.64 +0.1 960,236 - BWI76_RS04610 0.65 +0.1 960,254 - BWI76_RS04610 0.66 +0.6 960,445 + BWI76_RS04610 0.78 +0.9 960,448 - BWI76_RS04610 0.78 -0.6 960,506 - BWI76_RS04610 0.82 +0.6 960,555 + BWI76_RS04610 0.85 -0.1 960,909 - BWI76_RS04615 0.13 -1.3 961,024 + BWI76_RS04615 0.25 +0.5 961,132 - BWI76_RS04615 0.36 +0.2 961,132 - BWI76_RS04615 0.36 +0.1 961,321 + BWI76_RS04615 0.55 -0.2 961,329 - BWI76_RS04615 0.56 -0.0 961,495 + BWI76_RS04615 0.73 +1.0 961,674 - -0.5
Or see this region's nucleotide sequence