Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4833 and Psyr_4834 are separated by 102 nucleotides Psyr_4834 and Psyr_4835 are separated by 15 nucleotides
Psyr_4833: Psyr_4833 - histidine ammonia-lyase, at 5,736,159 to 5,737,706
_4833
Psyr_4834: Psyr_4834 - histidine:proton symporter, AAT family, at 5,737,809 to 5,739,185
_4834
Psyr_4835: Psyr_4835 - imidazolonepropionase, at 5,739,201 to 5,740,406
_4835
Position (kb)
5737
5738
5739
5740 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5737.495 kb on + strand, within Psyr_4833 at 5737.496 kb on - strand, within Psyr_4833 at 5737.496 kb on - strand, within Psyr_4833 at 5737.600 kb on - strand at 5737.600 kb on - strand at 5737.785 kb on + strand at 5737.786 kb on - strand at 5737.886 kb on + strand at 5737.899 kb on + strand at 5737.900 kb on - strand at 5737.939 kb on + strand at 5737.940 kb on - strand at 5737.950 kb on + strand, within Psyr_4834 at 5737.951 kb on - strand, within Psyr_4834 at 5737.974 kb on + strand, within Psyr_4834 at 5737.974 kb on + strand, within Psyr_4834 at 5737.974 kb on + strand, within Psyr_4834 at 5737.974 kb on + strand, within Psyr_4834 at 5737.974 kb on + strand, within Psyr_4834 at 5737.974 kb on + strand, within Psyr_4834 at 5737.974 kb on + strand, within Psyr_4834 at 5737.974 kb on + strand, within Psyr_4834 at 5737.975 kb on - strand, within Psyr_4834 at 5737.975 kb on - strand, within Psyr_4834 at 5737.975 kb on - strand, within Psyr_4834 at 5737.975 kb on - strand, within Psyr_4834 at 5737.975 kb on - strand, within Psyr_4834 at 5738.040 kb on + strand, within Psyr_4834 at 5738.053 kb on - strand, within Psyr_4834 at 5738.053 kb on - strand, within Psyr_4834 at 5738.053 kb on - strand, within Psyr_4834 at 5738.053 kb on - strand, within Psyr_4834 at 5738.091 kb on + strand, within Psyr_4834 at 5738.091 kb on + strand, within Psyr_4834 at 5738.092 kb on - strand, within Psyr_4834 at 5738.092 kb on - strand, within Psyr_4834 at 5738.092 kb on - strand, within Psyr_4834 at 5738.092 kb on - strand, within Psyr_4834 at 5738.092 kb on - strand, within Psyr_4834 at 5738.145 kb on + strand, within Psyr_4834 at 5738.145 kb on + strand, within Psyr_4834 at 5738.145 kb on + strand, within Psyr_4834 at 5738.145 kb on + strand, within Psyr_4834 at 5738.145 kb on + strand, within Psyr_4834 at 5738.145 kb on + strand, within Psyr_4834 at 5738.145 kb on + strand, within Psyr_4834 at 5738.145 kb on + strand, within Psyr_4834 at 5738.145 kb on + strand, within Psyr_4834 at 5738.145 kb on + strand, within Psyr_4834 at 5738.145 kb on + strand, within Psyr_4834 at 5738.145 kb on + strand, within Psyr_4834 at 5738.145 kb on + strand, within Psyr_4834 at 5738.145 kb on + strand, within Psyr_4834 at 5738.146 kb on - strand, within Psyr_4834 at 5738.146 kb on - strand, within Psyr_4834 at 5738.146 kb on - strand, within Psyr_4834 at 5738.146 kb on - strand, within Psyr_4834 at 5738.146 kb on - strand, within Psyr_4834 at 5738.146 kb on - strand, within Psyr_4834 at 5738.146 kb on - strand, within Psyr_4834 at 5738.146 kb on - strand, within Psyr_4834 at 5738.553 kb on + strand, within Psyr_4834 at 5738.553 kb on + strand, within Psyr_4834 at 5738.553 kb on + strand, within Psyr_4834 at 5738.553 kb on + strand, within Psyr_4834 at 5738.553 kb on + strand, within Psyr_4834 at 5738.554 kb on - strand, within Psyr_4834 at 5738.554 kb on - strand, within Psyr_4834 at 5738.586 kb on + strand, within Psyr_4834 at 5738.742 kb on + strand, within Psyr_4834 at 5738.742 kb on + strand, within Psyr_4834 at 5738.742 kb on + strand, within Psyr_4834 at 5738.742 kb on + strand, within Psyr_4834 at 5738.742 kb on + strand, within Psyr_4834 at 5738.742 kb on + strand, within Psyr_4834 at 5738.742 kb on + strand, within Psyr_4834 at 5738.742 kb on + strand, within Psyr_4834 at 5738.742 kb on + strand, within Psyr_4834 at 5738.743 kb on - strand, within Psyr_4834 at 5738.743 kb on - strand, within Psyr_4834 at 5738.743 kb on - strand, within Psyr_4834 at 5738.743 kb on - strand, within Psyr_4834 at 5738.743 kb on - strand, within Psyr_4834 at 5738.862 kb on + strand, within Psyr_4834 at 5738.863 kb on - strand, within Psyr_4834 at 5738.863 kb on - strand, within Psyr_4834 at 5738.863 kb on - strand, within Psyr_4834 at 5738.863 kb on - strand, within Psyr_4834 at 5738.863 kb on - strand, within Psyr_4834 at 5739.075 kb on + strand at 5739.142 kb on - strand at 5739.142 kb on - strand at 5739.253 kb on - strand at 5739.404 kb on + strand, within Psyr_4835 at 5739.404 kb on + strand, within Psyr_4835 at 5739.405 kb on - strand, within Psyr_4835 at 5739.405 kb on - strand, within Psyr_4835 at 5739.462 kb on + strand, within Psyr_4835 at 5739.492 kb on - strand, within Psyr_4835 at 5739.540 kb on + strand, within Psyr_4835 at 5739.540 kb on + strand, within Psyr_4835 at 5739.729 kb on + strand, within Psyr_4835 at 5739.750 kb on + strand, within Psyr_4835 at 5739.750 kb on + strand, within Psyr_4835 at 5739.750 kb on + strand, within Psyr_4835 at 5739.750 kb on + strand, within Psyr_4835 at 5739.751 kb on - strand, within Psyr_4835 at 5739.751 kb on - strand, within Psyr_4835 at 5739.751 kb on - strand, within Psyr_4835 at 5739.751 kb on - strand, within Psyr_4835 at 5739.751 kb on - strand, within Psyr_4835 at 5739.831 kb on + strand, within Psyr_4835 at 5739.832 kb on - strand, within Psyr_4835 at 5739.955 kb on - strand, within Psyr_4835
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 5,737,495 + Psyr_4833 0.86 -3.2 5,737,496 - Psyr_4833 0.86 -0.8 5,737,496 - Psyr_4833 0.86 -0.4 5,737,600 - +0.3 5,737,600 - +0.2 5,737,785 + -0.5 5,737,786 - +1.2 5,737,886 + +1.6 5,737,899 + +2.9 5,737,900 - +1.9 5,737,939 + +1.1 5,737,940 - +0.4 5,737,950 + Psyr_4834 0.10 +2.7 5,737,951 - Psyr_4834 0.10 +0.7 5,737,974 + Psyr_4834 0.12 +0.7 5,737,974 + Psyr_4834 0.12 +1.6 5,737,974 + Psyr_4834 0.12 +0.9 5,737,974 + Psyr_4834 0.12 +1.6 5,737,974 + Psyr_4834 0.12 +3.1 5,737,974 + Psyr_4834 0.12 +1.3 5,737,974 + Psyr_4834 0.12 +1.8 5,737,974 + Psyr_4834 0.12 +1.4 5,737,975 - Psyr_4834 0.12 +1.0 5,737,975 - Psyr_4834 0.12 +1.6 5,737,975 - Psyr_4834 0.12 +0.9 5,737,975 - Psyr_4834 0.12 +1.6 5,737,975 - Psyr_4834 0.12 +1.5 5,738,040 + Psyr_4834 0.17 +0.8 5,738,053 - Psyr_4834 0.18 +2.7 5,738,053 - Psyr_4834 0.18 +2.4 5,738,053 - Psyr_4834 0.18 +0.2 5,738,053 - Psyr_4834 0.18 +2.5 5,738,091 + Psyr_4834 0.20 +1.3 5,738,091 + Psyr_4834 0.20 +1.6 5,738,092 - Psyr_4834 0.21 -0.7 5,738,092 - Psyr_4834 0.21 +2.2 5,738,092 - Psyr_4834 0.21 +1.4 5,738,092 - Psyr_4834 0.21 +1.2 5,738,092 - Psyr_4834 0.21 +1.9 5,738,145 + Psyr_4834 0.24 +2.6 5,738,145 + Psyr_4834 0.24 -0.1 5,738,145 + Psyr_4834 0.24 +2.6 5,738,145 + Psyr_4834 0.24 +2.0 5,738,145 + Psyr_4834 0.24 +1.2 5,738,145 + Psyr_4834 0.24 +2.5 5,738,145 + Psyr_4834 0.24 +1.5 5,738,145 + Psyr_4834 0.24 +1.7 5,738,145 + Psyr_4834 0.24 +1.9 5,738,145 + Psyr_4834 0.24 +2.5 5,738,145 + Psyr_4834 0.24 +0.2 5,738,145 + Psyr_4834 0.24 +2.1 5,738,145 + Psyr_4834 0.24 +1.6 5,738,145 + Psyr_4834 0.24 +2.5 5,738,146 - Psyr_4834 0.24 +1.0 5,738,146 - Psyr_4834 0.24 +1.2 5,738,146 - Psyr_4834 0.24 +0.4 5,738,146 - Psyr_4834 0.24 +0.3 5,738,146 - Psyr_4834 0.24 +1.6 5,738,146 - Psyr_4834 0.24 +3.2 5,738,146 - Psyr_4834 0.24 +2.1 5,738,146 - Psyr_4834 0.24 +2.4 5,738,553 + Psyr_4834 0.54 +0.2 5,738,553 + Psyr_4834 0.54 +2.1 5,738,553 + Psyr_4834 0.54 +1.8 5,738,553 + Psyr_4834 0.54 +2.1 5,738,553 + Psyr_4834 0.54 +2.3 5,738,554 - Psyr_4834 0.54 +0.3 5,738,554 - Psyr_4834 0.54 +1.7 5,738,586 + Psyr_4834 0.56 +0.8 5,738,742 + Psyr_4834 0.68 +1.6 5,738,742 + Psyr_4834 0.68 +1.6 5,738,742 + Psyr_4834 0.68 +3.5 5,738,742 + Psyr_4834 0.68 +1.4 5,738,742 + Psyr_4834 0.68 +2.3 5,738,742 + Psyr_4834 0.68 +1.6 5,738,742 + Psyr_4834 0.68 +0.1 5,738,742 + Psyr_4834 0.68 +2.5 5,738,742 + Psyr_4834 0.68 +2.4 5,738,743 - Psyr_4834 0.68 +1.7 5,738,743 - Psyr_4834 0.68 -1.1 5,738,743 - Psyr_4834 0.68 +3.1 5,738,743 - Psyr_4834 0.68 -3.5 5,738,743 - Psyr_4834 0.68 +0.9 5,738,862 + Psyr_4834 0.76 +1.3 5,738,863 - Psyr_4834 0.77 +1.6 5,738,863 - Psyr_4834 0.77 +1.1 5,738,863 - Psyr_4834 0.77 +0.8 5,738,863 - Psyr_4834 0.77 +0.9 5,738,863 - Psyr_4834 0.77 -0.1 5,739,075 + +1.2 5,739,142 - +2.1 5,739,142 - +1.6 5,739,253 - +1.1 5,739,404 + Psyr_4835 0.17 +0.1 5,739,404 + Psyr_4835 0.17 +0.8 5,739,405 - Psyr_4835 0.17 -2.5 5,739,405 - Psyr_4835 0.17 +0.4 5,739,462 + Psyr_4835 0.22 -0.2 5,739,492 - Psyr_4835 0.24 -0.9 5,739,540 + Psyr_4835 0.28 -1.2 5,739,540 + Psyr_4835 0.28 +0.4 5,739,729 + Psyr_4835 0.44 -0.4 5,739,750 + Psyr_4835 0.46 -2.2 5,739,750 + Psyr_4835 0.46 -0.2 5,739,750 + Psyr_4835 0.46 -0.7 5,739,750 + Psyr_4835 0.46 -0.0 5,739,751 - Psyr_4835 0.46 +0.7 5,739,751 - Psyr_4835 0.46 -0.1 5,739,751 - Psyr_4835 0.46 +0.5 5,739,751 - Psyr_4835 0.46 -1.9 5,739,751 - Psyr_4835 0.46 +2.0 5,739,831 + Psyr_4835 0.52 -1.3 5,739,832 - Psyr_4835 0.52 +0.0 5,739,955 - Psyr_4835 0.63 -0.3
Or see this region's nucleotide sequence