Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4354 and Psyr_4355 are separated by 5 nucleotides Psyr_4355 and Psyr_4356 are separated by 147 nucleotides Psyr_4356 and Psyr_4357 are separated by 83 nucleotides
Psyr_4354: Psyr_4354 - DNA polymerase III, delta subunit, at 5,208,672 to 5,209,709
_4354
Psyr_4355: Psyr_4355 - hypothetical protein, at 5,209,715 to 5,209,927
_4355
Psyr_4356: Psyr_4356 - conserved hypothetical protein, at 5,210,075 to 5,210,230
_4356
Psyr_4357: Psyr_4357 - HopAJ2 protein, at 5,210,314 to 5,211,651
_4357
Position (kb) 5209
5210 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5209.708 kb on - strand at 5209.725 kb on + strand at 5209.725 kb on + strand at 5209.725 kb on + strand at 5209.725 kb on + strand at 5209.725 kb on + strand at 5209.725 kb on + strand at 5209.725 kb on + strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.729 kb on + strand at 5209.729 kb on + strand at 5209.729 kb on + strand at 5209.729 kb on + strand at 5209.730 kb on - strand at 5209.730 kb on - strand at 5209.730 kb on - strand at 5209.730 kb on - strand at 5209.811 kb on + strand, within Psyr_4355 at 5209.811 kb on + strand, within Psyr_4355 at 5209.811 kb on + strand, within Psyr_4355 at 5209.812 kb on - strand, within Psyr_4355 at 5209.812 kb on - strand, within Psyr_4355 at 5209.812 kb on - strand, within Psyr_4355 at 5209.818 kb on + strand, within Psyr_4355 at 5209.819 kb on - strand, within Psyr_4355 at 5209.953 kb on + strand at 5209.954 kb on - strand at 5209.954 kb on - strand at 5209.954 kb on - strand at 5209.979 kb on + strand at 5209.979 kb on + strand at 5209.979 kb on + strand at 5209.979 kb on + strand at 5209.979 kb on + strand at 5209.979 kb on + strand at 5209.979 kb on + strand at 5209.980 kb on - strand at 5209.980 kb on - strand at 5209.980 kb on - strand at 5209.983 kb on + strand at 5209.983 kb on + strand at 5209.984 kb on - strand at 5209.984 kb on - strand at 5209.984 kb on - strand at 5209.995 kb on + strand at 5209.995 kb on + strand at 5209.996 kb on - strand at 5209.996 kb on - strand at 5210.006 kb on + strand at 5210.049 kb on + strand at 5210.049 kb on + strand at 5210.049 kb on + strand at 5210.050 kb on - strand at 5210.050 kb on - strand at 5210.050 kb on - strand at 5210.050 kb on - strand at 5210.194 kb on + strand, within Psyr_4356 at 5210.194 kb on + strand, within Psyr_4356 at 5210.195 kb on - strand, within Psyr_4356 at 5210.218 kb on + strand at 5210.219 kb on - strand at 5210.289 kb on - strand at 5210.297 kb on + strand at 5210.297 kb on + strand at 5210.582 kb on + strand, within Psyr_4357 at 5210.582 kb on + strand, within Psyr_4357 at 5210.583 kb on - strand, within Psyr_4357 at 5210.583 kb on - strand, within Psyr_4357 at 5210.675 kb on - strand, within Psyr_4357 at 5210.675 kb on - strand, within Psyr_4357 at 5210.734 kb on + strand, within Psyr_4357 at 5210.767 kb on + strand, within Psyr_4357 at 5210.815 kb on - strand, within Psyr_4357 at 5210.872 kb on + strand, within Psyr_4357 at 5210.872 kb on + strand, within Psyr_4357 at 5210.873 kb on - strand, within Psyr_4357
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 5,209,708 - -0.8 5,209,725 + -2.8 5,209,725 + -0.9 5,209,725 + +0.5 5,209,725 + -2.4 5,209,725 + -2.9 5,209,725 + -0.8 5,209,725 + -1.0 5,209,726 - -2.0 5,209,726 - -1.6 5,209,726 - -0.3 5,209,726 - -0.7 5,209,726 - +0.1 5,209,726 - +1.1 5,209,726 - +0.6 5,209,726 - -0.3 5,209,726 - -0.8 5,209,729 + -0.8 5,209,729 + -1.3 5,209,729 + -1.6 5,209,729 + +1.1 5,209,730 - -1.0 5,209,730 - -1.0 5,209,730 - -0.1 5,209,730 - -1.5 5,209,811 + Psyr_4355 0.45 -1.7 5,209,811 + Psyr_4355 0.45 +1.0 5,209,811 + Psyr_4355 0.45 -0.4 5,209,812 - Psyr_4355 0.46 -2.7 5,209,812 - Psyr_4355 0.46 -1.5 5,209,812 - Psyr_4355 0.46 -2.0 5,209,818 + Psyr_4355 0.48 +0.6 5,209,819 - Psyr_4355 0.49 -0.4 5,209,953 + -1.5 5,209,954 - -2.1 5,209,954 - -0.7 5,209,954 - -1.3 5,209,979 + +0.9 5,209,979 + -0.8 5,209,979 + -1.4 5,209,979 + -2.0 5,209,979 + +0.9 5,209,979 + -1.0 5,209,979 + -0.6 5,209,980 - -1.3 5,209,980 - -0.6 5,209,980 - -1.0 5,209,983 + +0.1 5,209,983 + -1.4 5,209,984 - +1.1 5,209,984 - +2.0 5,209,984 - -0.8 5,209,995 + +0.0 5,209,995 + -0.7 5,209,996 - -0.4 5,209,996 - -1.6 5,210,006 + -0.4 5,210,049 + -1.2 5,210,049 + -0.3 5,210,049 + -0.6 5,210,050 - -0.5 5,210,050 - +0.2 5,210,050 - -0.3 5,210,050 - -0.1 5,210,194 + Psyr_4356 0.76 -0.1 5,210,194 + Psyr_4356 0.76 -2.4 5,210,195 - Psyr_4356 0.77 -0.1 5,210,218 + -2.2 5,210,219 - -1.2 5,210,289 - -1.3 5,210,297 + -1.2 5,210,297 + +0.2 5,210,582 + Psyr_4357 0.20 -0.9 5,210,582 + Psyr_4357 0.20 -1.2 5,210,583 - Psyr_4357 0.20 +0.1 5,210,583 - Psyr_4357 0.20 -0.7 5,210,675 - Psyr_4357 0.27 -1.6 5,210,675 - Psyr_4357 0.27 +0.3 5,210,734 + Psyr_4357 0.31 -0.1 5,210,767 + Psyr_4357 0.34 -0.2 5,210,815 - Psyr_4357 0.37 -1.2 5,210,872 + Psyr_4357 0.42 +1.5 5,210,872 + Psyr_4357 0.42 +1.7 5,210,873 - Psyr_4357 0.42 -0.9
Or see this region's nucleotide sequence