Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3741 and Psyr_3742 are separated by 148 nucleotides Psyr_3742 and Psyr_3743 are separated by 202 nucleotides Psyr_3743 and Psyr_3744 are separated by 1 nucleotides
Psyr_3741: Psyr_3741 - Polyphosphate:AMP phosphotransferase, at 4,455,375 to 4,456,871
_3741
Psyr_3742: Psyr_3742 - FAD dependent oxidoreductase:Protein of unknown function DUF752, at 4,457,020 to 4,459,017
_3742
Psyr_3743: Psyr_3743 - ABC transporter, at 4,459,220 to 4,459,888
_3743
Psyr_3744: Psyr_3744 - Protein of unknown function DUF214, at 4,459,890 to 4,462,370
_3744
Position (kb)
4457
4458
4459
4460 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4456.220 kb on - strand, within Psyr_3741 at 4456.313 kb on - strand, within Psyr_3741 at 4456.566 kb on - strand, within Psyr_3741 at 4456.566 kb on - strand, within Psyr_3741 at 4456.652 kb on + strand, within Psyr_3741 at 4456.652 kb on + strand, within Psyr_3741 at 4456.725 kb on + strand at 4456.725 kb on + strand at 4456.725 kb on + strand at 4456.725 kb on + strand at 4456.725 kb on + strand at 4456.725 kb on + strand at 4456.725 kb on + strand at 4456.725 kb on + strand at 4456.725 kb on + strand at 4456.726 kb on - strand at 4456.726 kb on - strand at 4456.726 kb on - strand at 4456.726 kb on - strand at 4456.726 kb on - strand at 4456.726 kb on - strand at 4456.726 kb on - strand at 4456.726 kb on - strand at 4456.776 kb on + strand at 4456.776 kb on + strand at 4456.777 kb on - strand at 4456.777 kb on - strand at 4456.777 kb on - strand at 4456.825 kb on + strand at 4456.825 kb on + strand at 4456.826 kb on - strand at 4457.095 kb on + strand at 4457.392 kb on + strand, within Psyr_3742 at 4457.392 kb on + strand, within Psyr_3742 at 4457.392 kb on + strand, within Psyr_3742 at 4457.392 kb on + strand, within Psyr_3742 at 4457.392 kb on + strand, within Psyr_3742 at 4457.393 kb on - strand, within Psyr_3742 at 4457.393 kb on - strand, within Psyr_3742 at 4457.393 kb on - strand, within Psyr_3742 at 4457.451 kb on + strand, within Psyr_3742 at 4457.451 kb on + strand, within Psyr_3742 at 4457.452 kb on - strand, within Psyr_3742 at 4457.452 kb on - strand, within Psyr_3742 at 4457.452 kb on - strand, within Psyr_3742 at 4457.452 kb on - strand, within Psyr_3742 at 4457.452 kb on - strand, within Psyr_3742 at 4457.452 kb on - strand, within Psyr_3742 at 4457.613 kb on + strand, within Psyr_3742 at 4457.614 kb on - strand, within Psyr_3742 at 4457.614 kb on - strand, within Psyr_3742 at 4457.614 kb on - strand, within Psyr_3742 at 4457.614 kb on - strand, within Psyr_3742 at 4457.620 kb on - strand, within Psyr_3742 at 4457.620 kb on - strand, within Psyr_3742 at 4457.772 kb on - strand, within Psyr_3742 at 4457.941 kb on + strand, within Psyr_3742 at 4457.941 kb on + strand, within Psyr_3742 at 4457.941 kb on + strand, within Psyr_3742 at 4457.941 kb on + strand, within Psyr_3742 at 4457.942 kb on - strand, within Psyr_3742 at 4457.942 kb on - strand, within Psyr_3742 at 4457.942 kb on - strand, within Psyr_3742 at 4457.942 kb on - strand, within Psyr_3742 at 4458.388 kb on - strand, within Psyr_3742 at 4458.505 kb on + strand, within Psyr_3742 at 4458.506 kb on - strand, within Psyr_3742 at 4458.506 kb on - strand, within Psyr_3742 at 4458.506 kb on - strand, within Psyr_3742 at 4458.534 kb on + strand, within Psyr_3742 at 4458.766 kb on + strand, within Psyr_3742 at 4458.832 kb on + strand at 4458.833 kb on - strand at 4458.833 kb on - strand at 4458.833 kb on - strand at 4458.838 kb on - strand at 4459.066 kb on + strand at 4459.066 kb on + strand at 4459.158 kb on + strand at 4459.239 kb on + strand at 4459.240 kb on - strand at 4459.251 kb on - strand at 4459.659 kb on + strand, within Psyr_3743 at 4459.660 kb on - strand, within Psyr_3743 at 4459.750 kb on - strand, within Psyr_3743 at 4459.964 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 4,456,220 - Psyr_3741 0.56 -1.1 4,456,313 - Psyr_3741 0.63 +1.3 4,456,566 - Psyr_3741 0.80 -0.3 4,456,566 - Psyr_3741 0.80 +0.4 4,456,652 + Psyr_3741 0.85 +1.5 4,456,652 + Psyr_3741 0.85 -0.6 4,456,725 + -0.7 4,456,725 + -1.0 4,456,725 + -0.5 4,456,725 + -1.3 4,456,725 + -1.4 4,456,725 + +0.7 4,456,725 + -2.0 4,456,725 + +1.7 4,456,725 + +1.5 4,456,726 - +0.8 4,456,726 - +0.5 4,456,726 - +2.0 4,456,726 - -0.5 4,456,726 - -1.7 4,456,726 - -1.2 4,456,726 - -1.2 4,456,726 - +0.5 4,456,776 + -0.6 4,456,776 + -0.5 4,456,777 - +0.1 4,456,777 - -1.1 4,456,777 - +1.1 4,456,825 + -1.5 4,456,825 + -1.1 4,456,826 - +0.6 4,457,095 + -0.6 4,457,392 + Psyr_3742 0.19 -0.9 4,457,392 + Psyr_3742 0.19 +0.8 4,457,392 + Psyr_3742 0.19 +0.4 4,457,392 + Psyr_3742 0.19 +0.2 4,457,392 + Psyr_3742 0.19 +1.0 4,457,393 - Psyr_3742 0.19 +0.7 4,457,393 - Psyr_3742 0.19 -0.5 4,457,393 - Psyr_3742 0.19 -2.2 4,457,451 + Psyr_3742 0.22 -0.7 4,457,451 + Psyr_3742 0.22 +0.3 4,457,452 - Psyr_3742 0.22 -0.9 4,457,452 - Psyr_3742 0.22 +0.5 4,457,452 - Psyr_3742 0.22 +1.4 4,457,452 - Psyr_3742 0.22 +0.1 4,457,452 - Psyr_3742 0.22 -1.8 4,457,452 - Psyr_3742 0.22 +0.3 4,457,613 + Psyr_3742 0.30 +0.4 4,457,614 - Psyr_3742 0.30 -0.6 4,457,614 - Psyr_3742 0.30 +1.3 4,457,614 - Psyr_3742 0.30 +0.1 4,457,614 - Psyr_3742 0.30 +1.3 4,457,620 - Psyr_3742 0.30 -0.2 4,457,620 - Psyr_3742 0.30 +0.9 4,457,772 - Psyr_3742 0.38 +1.4 4,457,941 + Psyr_3742 0.46 +0.1 4,457,941 + Psyr_3742 0.46 +0.8 4,457,941 + Psyr_3742 0.46 -0.4 4,457,941 + Psyr_3742 0.46 +0.8 4,457,942 - Psyr_3742 0.46 +2.3 4,457,942 - Psyr_3742 0.46 +0.3 4,457,942 - Psyr_3742 0.46 -0.8 4,457,942 - Psyr_3742 0.46 -0.8 4,458,388 - Psyr_3742 0.68 -0.6 4,458,505 + Psyr_3742 0.74 +0.3 4,458,506 - Psyr_3742 0.74 +0.3 4,458,506 - Psyr_3742 0.74 +3.1 4,458,506 - Psyr_3742 0.74 +0.4 4,458,534 + Psyr_3742 0.76 -0.5 4,458,766 + Psyr_3742 0.87 +0.2 4,458,832 + -0.8 4,458,833 - +0.1 4,458,833 - +0.1 4,458,833 - +0.9 4,458,838 - +0.4 4,459,066 + -1.5 4,459,066 + +0.1 4,459,158 + -2.4 4,459,239 + -0.1 4,459,240 - +2.1 4,459,251 - -0.5 4,459,659 + Psyr_3743 0.66 +1.3 4,459,660 - Psyr_3743 0.66 -0.3 4,459,750 - Psyr_3743 0.79 +0.7 4,459,964 - -0.7
Or see this region's nucleotide sequence