Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2655 and Psyr_2656 are separated by 171 nucleotides Psyr_2656 and Psyr_2657 are separated by 143 nucleotides Psyr_2657 and Psyr_2658 are separated by 197 nucleotides
Psyr_2655: Psyr_2655 - Nitroreductase, at 3,225,794 to 3,226,615
_2655
Psyr_2656: Psyr_2656 - hypothetical protein, at 3,226,787 to 3,227,137
_2656
Psyr_2657: Psyr_2657 - hypothetical protein, at 3,227,281 to 3,228,120
_2657
Psyr_2658: Psyr_2658 - hypothetical protein, at 3,228,318 to 3,229,451
_2658
Position (kb) 3227
3228
3229 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 3226.347 kb on + strand, within Psyr_2655 at 3226.425 kb on - strand, within Psyr_2655 at 3226.459 kb on - strand, within Psyr_2655 at 3226.459 kb on - strand, within Psyr_2655 at 3226.460 kb on + strand, within Psyr_2655 at 3226.460 kb on + strand, within Psyr_2655 at 3226.460 kb on + strand, within Psyr_2655 at 3226.460 kb on + strand, within Psyr_2655 at 3226.461 kb on - strand, within Psyr_2655 at 3226.461 kb on - strand, within Psyr_2655 at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.702 kb on - strand at 3226.702 kb on - strand at 3226.702 kb on - strand at 3226.702 kb on - strand at 3226.702 kb on - strand at 3226.727 kb on - strand at 3226.727 kb on - strand at 3226.757 kb on + strand at 3226.758 kb on - strand at 3226.758 kb on - strand at 3226.768 kb on - strand at 3226.771 kb on + strand at 3226.771 kb on + strand at 3226.771 kb on + strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.788 kb on + strand at 3226.789 kb on - strand at 3226.841 kb on - strand, within Psyr_2656 at 3226.841 kb on - strand, within Psyr_2656 at 3226.863 kb on + strand, within Psyr_2656 at 3226.938 kb on + strand, within Psyr_2656 at 3226.938 kb on + strand, within Psyr_2656 at 3226.939 kb on - strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.946 kb on + strand, within Psyr_2656 at 3226.946 kb on + strand, within Psyr_2656 at 3226.946 kb on + strand, within Psyr_2656 at 3226.946 kb on + strand, within Psyr_2656 at 3226.947 kb on - strand, within Psyr_2656 at 3226.947 kb on - strand, within Psyr_2656 at 3227.060 kb on + strand, within Psyr_2656 at 3227.061 kb on - strand, within Psyr_2656 at 3227.061 kb on - strand, within Psyr_2656 at 3227.149 kb on + strand at 3227.149 kb on + strand at 3227.149 kb on + strand at 3227.149 kb on + strand at 3227.149 kb on + strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.204 kb on + strand at 3227.204 kb on + strand at 3227.205 kb on - strand at 3227.205 kb on - strand at 3227.205 kb on - strand at 3227.306 kb on - strand at 3227.306 kb on - strand at 3227.306 kb on - strand at 3227.377 kb on + strand, within Psyr_2657 at 3227.377 kb on + strand at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.421 kb on - strand, within Psyr_2657 at 3227.451 kb on + strand, within Psyr_2657 at 3227.452 kb on - strand, within Psyr_2657 at 3227.503 kb on + strand, within Psyr_2657 at 3227.504 kb on - strand, within Psyr_2657 at 3227.617 kb on + strand, within Psyr_2657 at 3227.617 kb on + strand, within Psyr_2657 at 3227.617 kb on + strand, within Psyr_2657 at 3227.618 kb on - strand, within Psyr_2657 at 3227.618 kb on - strand, within Psyr_2657 at 3227.618 kb on - strand, within Psyr_2657 at 3227.705 kb on + strand, within Psyr_2657 at 3227.706 kb on - strand, within Psyr_2657 at 3227.803 kb on + strand, within Psyr_2657 at 3227.803 kb on + strand, within Psyr_2657 at 3227.817 kb on + strand, within Psyr_2657 at 3227.817 kb on + strand, within Psyr_2657 at 3227.817 kb on + strand, within Psyr_2657 at 3227.817 kb on + strand, within Psyr_2657 at 3227.817 kb on + strand, within Psyr_2657 at 3227.817 kb on + strand, within Psyr_2657 at 3227.817 kb on + strand, within Psyr_2657 at 3227.817 kb on + strand, within Psyr_2657 at 3227.817 kb on + strand, within Psyr_2657 at 3227.818 kb on - strand, within Psyr_2657 at 3227.818 kb on - strand, within Psyr_2657 at 3227.818 kb on - strand, within Psyr_2657 at 3227.818 kb on - strand, within Psyr_2657 at 3227.818 kb on - strand, within Psyr_2657 at 3227.818 kb on - strand, within Psyr_2657 at 3227.818 kb on - strand, within Psyr_2657 at 3227.829 kb on + strand, within Psyr_2657 at 3227.830 kb on - strand, within Psyr_2657 at 3227.830 kb on - strand, within Psyr_2657 at 3227.872 kb on - strand, within Psyr_2657 at 3227.872 kb on - strand, within Psyr_2657 at 3227.899 kb on - strand, within Psyr_2657 at 3228.013 kb on + strand, within Psyr_2657 at 3228.013 kb on + strand, within Psyr_2657 at 3228.027 kb on + strand, within Psyr_2657 at 3228.027 kb on + strand, within Psyr_2657 at 3228.028 kb on - strand, within Psyr_2657 at 3228.028 kb on - strand, within Psyr_2657 at 3228.063 kb on + strand at 3228.064 kb on - strand at 3228.160 kb on + strand at 3228.160 kb on + strand at 3228.161 kb on - strand at 3228.161 kb on - strand at 3228.223 kb on - strand at 3228.223 kb on - strand at 3228.232 kb on + strand at 3228.232 kb on + strand at 3228.232 kb on + strand at 3228.232 kb on + strand at 3228.232 kb on + strand at 3228.233 kb on - strand at 3228.233 kb on - strand at 3228.233 kb on - strand at 3228.233 kb on - strand at 3228.233 kb on - strand at 3228.233 kb on - strand at 3228.233 kb on - strand at 3228.233 kb on - strand at 3228.268 kb on + strand at 3228.268 kb on + strand at 3228.268 kb on + strand at 3228.268 kb on + strand at 3228.268 kb on + strand at 3228.268 kb on + strand at 3228.268 kb on + strand at 3228.269 kb on - strand at 3228.269 kb on - strand at 3228.269 kb on - strand at 3228.269 kb on - strand at 3228.269 kb on - strand at 3228.269 kb on - strand at 3228.269 kb on - strand at 3228.269 kb on - strand at 3228.269 kb on - strand at 3228.269 kb on - strand at 3228.304 kb on + strand at 3228.304 kb on + strand at 3228.304 kb on + strand at 3228.304 kb on + strand at 3228.304 kb on + strand at 3228.305 kb on - strand at 3228.305 kb on - strand at 3228.305 kb on - strand at 3228.305 kb on - strand at 3228.308 kb on + strand at 3228.368 kb on - strand at 3228.368 kb on - strand at 3228.405 kb on - strand at 3228.405 kb on - strand at 3228.405 kb on - strand at 3228.418 kb on + strand at 3228.418 kb on + strand at 3228.418 kb on + strand at 3228.418 kb on + strand at 3228.418 kb on + strand at 3228.419 kb on - strand at 3228.419 kb on - strand at 3228.421 kb on + strand at 3228.421 kb on + strand at 3228.421 kb on + strand at 3228.421 kb on + strand at 3228.421 kb on + strand at 3228.421 kb on + strand at 3228.422 kb on - strand at 3228.553 kb on + strand, within Psyr_2658 at 3228.554 kb on - strand, within Psyr_2658 at 3228.554 kb on - strand, within Psyr_2658 at 3228.584 kb on + strand, within Psyr_2658 at 3228.613 kb on + strand, within Psyr_2658 at 3228.830 kb on - strand, within Psyr_2658 at 3228.830 kb on - strand, within Psyr_2658 at 3228.873 kb on - strand, within Psyr_2658 at 3228.881 kb on - strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.977 kb on - strand, within Psyr_2658 at 3228.977 kb on - strand, within Psyr_2658 at 3228.977 kb on - strand, within Psyr_2658 at 3228.998 kb on + strand, within Psyr_2658 at 3228.999 kb on - strand, within Psyr_2658 at 3229.027 kb on + strand, within Psyr_2658 at 3229.028 kb on - strand, within Psyr_2658 at 3229.028 kb on - strand, within Psyr_2658 at 3229.028 kb on - strand, within Psyr_2658 at 3229.089 kb on + strand, within Psyr_2658
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 3,226,347 + Psyr_2655 0.67 -1.4 3,226,425 - Psyr_2655 0.77 +0.6 3,226,459 - Psyr_2655 0.81 +2.2 3,226,459 - Psyr_2655 0.81 +0.2 3,226,460 + Psyr_2655 0.81 -0.6 3,226,460 + Psyr_2655 0.81 +1.5 3,226,460 + Psyr_2655 0.81 -0.1 3,226,460 + Psyr_2655 0.81 +0.8 3,226,461 - Psyr_2655 0.81 +1.0 3,226,461 - Psyr_2655 0.81 +0.3 3,226,701 + -1.1 3,226,701 + -0.7 3,226,701 + -0.5 3,226,701 + -1.4 3,226,701 + +1.1 3,226,701 + -0.4 3,226,701 + -0.8 3,226,701 + +1.2 3,226,702 - +0.6 3,226,702 - +1.1 3,226,702 - -0.1 3,226,702 - +1.5 3,226,702 - -1.3 3,226,727 - -1.1 3,226,727 - +0.1 3,226,757 + -0.8 3,226,758 - +0.8 3,226,758 - +2.2 3,226,768 - -2.1 3,226,771 + -0.9 3,226,771 + +0.9 3,226,771 + +1.6 3,226,772 - -1.6 3,226,772 - -1.2 3,226,772 - -1.7 3,226,772 - -1.8 3,226,772 - +0.3 3,226,772 - +0.8 3,226,788 + +0.7 3,226,789 - -0.4 3,226,841 - Psyr_2656 0.15 +1.2 3,226,841 - Psyr_2656 0.15 +1.0 3,226,863 + Psyr_2656 0.22 -2.0 3,226,938 + Psyr_2656 0.43 -3.1 3,226,938 + Psyr_2656 0.43 -1.4 3,226,939 - Psyr_2656 0.43 +1.2 3,226,942 + Psyr_2656 0.44 -1.1 3,226,942 + Psyr_2656 0.44 +1.5 3,226,942 + Psyr_2656 0.44 +0.6 3,226,942 + Psyr_2656 0.44 +2.2 3,226,942 + Psyr_2656 0.44 -1.1 3,226,942 + Psyr_2656 0.44 -0.1 3,226,943 - Psyr_2656 0.44 +0.7 3,226,943 - Psyr_2656 0.44 +2.9 3,226,943 - Psyr_2656 0.44 +1.3 3,226,943 - Psyr_2656 0.44 -0.4 3,226,943 - Psyr_2656 0.44 -0.4 3,226,943 - Psyr_2656 0.44 -1.0 3,226,946 + Psyr_2656 0.45 -2.2 3,226,946 + Psyr_2656 0.45 +0.8 3,226,946 + Psyr_2656 0.45 -0.0 3,226,946 + Psyr_2656 0.45 -2.1 3,226,947 - Psyr_2656 0.46 +0.7 3,226,947 - Psyr_2656 0.46 +1.2 3,227,060 + Psyr_2656 0.78 +0.8 3,227,061 - Psyr_2656 0.78 -0.7 3,227,061 - Psyr_2656 0.78 -0.8 3,227,149 + -1.4 3,227,149 + +0.9 3,227,149 + -1.4 3,227,149 + -1.3 3,227,149 + +0.2 3,227,150 - +1.8 3,227,150 - -2.4 3,227,150 - +1.9 3,227,150 - -0.3 3,227,150 - -1.0 3,227,150 - +0.5 3,227,150 - -0.1 3,227,204 + +0.4 3,227,204 + +0.4 3,227,205 - +1.4 3,227,205 - -0.0 3,227,205 - -0.5 3,227,306 - -1.4 3,227,306 - -0.7 3,227,306 - +0.0 3,227,377 + Psyr_2657 0.11 -1.1 3,227,377 + -0.4 3,227,378 - Psyr_2657 0.12 -0.7 3,227,378 - Psyr_2657 0.12 +0.7 3,227,378 - Psyr_2657 0.12 -1.6 3,227,378 - Psyr_2657 0.12 -0.5 3,227,378 - Psyr_2657 0.12 +0.2 3,227,378 - Psyr_2657 0.12 +0.7 3,227,421 - Psyr_2657 0.17 -1.7 3,227,451 + Psyr_2657 0.20 -2.0 3,227,452 - Psyr_2657 0.20 -0.5 3,227,503 + Psyr_2657 0.26 -1.8 3,227,504 - Psyr_2657 0.27 +0.0 3,227,617 + Psyr_2657 0.40 -0.9 3,227,617 + Psyr_2657 0.40 -0.6 3,227,617 + Psyr_2657 0.40 -0.2 3,227,618 - Psyr_2657 0.40 -1.6 3,227,618 - Psyr_2657 0.40 +1.2 3,227,618 - Psyr_2657 0.40 -0.8 3,227,705 + Psyr_2657 0.50 +0.5 3,227,706 - Psyr_2657 0.51 +1.6 3,227,803 + Psyr_2657 0.62 -2.2 3,227,803 + Psyr_2657 0.62 -0.8 3,227,817 + Psyr_2657 0.64 -2.6 3,227,817 + Psyr_2657 0.64 -1.6 3,227,817 + Psyr_2657 0.64 -0.0 3,227,817 + Psyr_2657 0.64 -1.6 3,227,817 + Psyr_2657 0.64 +0.3 3,227,817 + Psyr_2657 0.64 +0.3 3,227,817 + Psyr_2657 0.64 +0.3 3,227,817 + Psyr_2657 0.64 -1.5 3,227,817 + Psyr_2657 0.64 +1.2 3,227,818 - Psyr_2657 0.64 -1.4 3,227,818 - Psyr_2657 0.64 +0.7 3,227,818 - Psyr_2657 0.64 -2.8 3,227,818 - Psyr_2657 0.64 +0.1 3,227,818 - Psyr_2657 0.64 +0.6 3,227,818 - Psyr_2657 0.64 -1.4 3,227,818 - Psyr_2657 0.64 -2.0 3,227,829 + Psyr_2657 0.65 -0.6 3,227,830 - Psyr_2657 0.65 -1.1 3,227,830 - Psyr_2657 0.65 -2.0 3,227,872 - Psyr_2657 0.70 +1.2 3,227,872 - Psyr_2657 0.70 -3.4 3,227,899 - Psyr_2657 0.74 -0.8 3,228,013 + Psyr_2657 0.87 -2.7 3,228,013 + Psyr_2657 0.87 -0.2 3,228,027 + Psyr_2657 0.89 -1.7 3,228,027 + Psyr_2657 0.89 -2.5 3,228,028 - Psyr_2657 0.89 -2.3 3,228,028 - Psyr_2657 0.89 -0.1 3,228,063 + -0.5 3,228,064 - -1.9 3,228,160 + +1.2 3,228,160 + +2.5 3,228,161 - -0.9 3,228,161 - +1.2 3,228,223 - +0.0 3,228,223 - -0.9 3,228,232 + +2.2 3,228,232 + +0.2 3,228,232 + -2.6 3,228,232 + -3.3 3,228,232 + -0.6 3,228,233 - -1.3 3,228,233 - +1.0 3,228,233 - -2.1 3,228,233 - +0.4 3,228,233 - -0.4 3,228,233 - +2.8 3,228,233 - -1.0 3,228,233 - +0.1 3,228,268 + -0.9 3,228,268 + -2.7 3,228,268 + -1.4 3,228,268 + -3.7 3,228,268 + -1.7 3,228,268 + -0.6 3,228,268 + -1.0 3,228,269 - -3.1 3,228,269 - -0.9 3,228,269 - +2.2 3,228,269 - -2.5 3,228,269 - -0.6 3,228,269 - -0.8 3,228,269 - +1.9 3,228,269 - -1.6 3,228,269 - -0.3 3,228,269 - -2.2 3,228,304 + -2.4 3,228,304 + -2.1 3,228,304 + -1.6 3,228,304 + -1.3 3,228,304 + +0.2 3,228,305 - -3.0 3,228,305 - +0.5 3,228,305 - -2.6 3,228,305 - -1.4 3,228,308 + -1.1 3,228,368 - -2.3 3,228,368 - +1.6 3,228,405 - +0.0 3,228,405 - -1.4 3,228,405 - -0.4 3,228,418 + -3.1 3,228,418 + -1.2 3,228,418 + +1.2 3,228,418 + -0.1 3,228,418 + -2.1 3,228,419 - +0.5 3,228,419 - +0.2 3,228,421 + -2.7 3,228,421 + -1.3 3,228,421 + -1.6 3,228,421 + -2.6 3,228,421 + -2.8 3,228,421 + -1.1 3,228,422 - -1.0 3,228,553 + Psyr_2658 0.21 -2.8 3,228,554 - Psyr_2658 0.21 -1.1 3,228,554 - Psyr_2658 0.21 -0.0 3,228,584 + Psyr_2658 0.23 +0.5 3,228,613 + Psyr_2658 0.26 -1.6 3,228,830 - Psyr_2658 0.45 -2.2 3,228,830 - Psyr_2658 0.45 +1.5 3,228,873 - Psyr_2658 0.49 -2.3 3,228,881 - Psyr_2658 0.50 +0.9 3,228,976 + Psyr_2658 0.58 +1.7 3,228,976 + Psyr_2658 0.58 -0.8 3,228,976 + Psyr_2658 0.58 -1.5 3,228,976 + Psyr_2658 0.58 +0.6 3,228,976 + Psyr_2658 0.58 -0.6 3,228,976 + Psyr_2658 0.58 -0.2 3,228,976 + Psyr_2658 0.58 -1.4 3,228,976 + Psyr_2658 0.58 +1.0 3,228,976 + Psyr_2658 0.58 -0.0 3,228,976 + Psyr_2658 0.58 -2.0 3,228,977 - Psyr_2658 0.58 -0.8 3,228,977 - Psyr_2658 0.58 -0.6 3,228,977 - Psyr_2658 0.58 -1.8 3,228,998 + Psyr_2658 0.60 +0.6 3,228,999 - Psyr_2658 0.60 -1.1 3,229,027 + Psyr_2658 0.63 -1.0 3,229,028 - Psyr_2658 0.63 +0.1 3,229,028 - Psyr_2658 0.63 -1.1 3,229,028 - Psyr_2658 0.63 +0.8 3,229,089 + Psyr_2658 0.68 -1.0
Or see this region's nucleotide sequence