Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2623 and Psyr_2624 are separated by 55 nucleotides Psyr_2624 and Psyr_2625 are separated by 7 nucleotides Psyr_2625 and Psyr_2626 are separated by 32 nucleotides
Psyr_2623: Psyr_2623 - conserved hypothetical protein, at 3,182,993 to 3,184,252
_2623
Psyr_2624: Psyr_2624 - lipoprotein, putative, at 3,184,308 to 3,184,808
_2624
Psyr_2625: Psyr_2625 - lipoprotein, putative, at 3,184,816 to 3,185,553
_2625
Psyr_2626: Psyr_2626 - Protein of unknown function DUF323, at 3,185,586 to 3,187,133
_2626
Position (kb)
3184
3185
3186 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3183.904 kb on + strand, within Psyr_2623 at 3183.904 kb on + strand, within Psyr_2623 at 3183.904 kb on + strand, within Psyr_2623 at 3183.905 kb on - strand, within Psyr_2623 at 3183.906 kb on + strand, within Psyr_2623 at 3183.907 kb on - strand, within Psyr_2623 at 3183.949 kb on + strand, within Psyr_2623 at 3183.968 kb on - strand, within Psyr_2623 at 3184.298 kb on + strand at 3184.302 kb on + strand at 3184.303 kb on - strand at 3184.327 kb on - strand at 3184.327 kb on - strand at 3184.327 kb on - strand at 3184.327 kb on - strand at 3184.597 kb on + strand, within Psyr_2624 at 3184.799 kb on + strand at 3184.802 kb on + strand at 3184.803 kb on - strand at 3184.848 kb on - strand at 3184.938 kb on + strand, within Psyr_2625 at 3184.970 kb on + strand, within Psyr_2625 at 3184.970 kb on + strand, within Psyr_2625 at 3184.979 kb on - strand, within Psyr_2625 at 3185.007 kb on + strand, within Psyr_2625 at 3185.144 kb on + strand, within Psyr_2625 at 3185.144 kb on + strand, within Psyr_2625 at 3185.145 kb on - strand, within Psyr_2625 at 3185.145 kb on - strand, within Psyr_2625 at 3185.145 kb on - strand, within Psyr_2625 at 3185.148 kb on + strand, within Psyr_2625 at 3185.149 kb on - strand, within Psyr_2625 at 3185.179 kb on + strand, within Psyr_2625 at 3185.222 kb on + strand, within Psyr_2625 at 3185.222 kb on + strand, within Psyr_2625 at 3185.223 kb on - strand, within Psyr_2625 at 3185.243 kb on + strand, within Psyr_2625 at 3185.243 kb on + strand, within Psyr_2625 at 3185.243 kb on + strand, within Psyr_2625 at 3185.244 kb on - strand, within Psyr_2625 at 3185.246 kb on + strand, within Psyr_2625 at 3185.246 kb on + strand, within Psyr_2625 at 3185.249 kb on + strand, within Psyr_2625 at 3185.249 kb on + strand, within Psyr_2625 at 3185.249 kb on + strand, within Psyr_2625 at 3185.249 kb on + strand, within Psyr_2625 at 3185.249 kb on + strand, within Psyr_2625 at 3185.250 kb on - strand, within Psyr_2625 at 3185.250 kb on - strand, within Psyr_2625 at 3185.343 kb on + strand, within Psyr_2625 at 3185.347 kb on + strand, within Psyr_2625 at 3185.542 kb on + strand at 3185.543 kb on - strand at 3185.543 kb on - strand at 3185.543 kb on - strand at 3185.543 kb on - strand at 3185.543 kb on - strand at 3185.543 kb on - strand at 3185.587 kb on + strand at 3185.601 kb on - strand at 3185.628 kb on + strand at 3185.629 kb on - strand at 3185.781 kb on + strand, within Psyr_2626 at 3185.797 kb on + strand, within Psyr_2626 at 3185.849 kb on + strand, within Psyr_2626 at 3185.899 kb on + strand, within Psyr_2626 at 3185.923 kb on + strand, within Psyr_2626 at 3185.923 kb on + strand, within Psyr_2626 at 3185.923 kb on + strand, within Psyr_2626 at 3185.923 kb on + strand, within Psyr_2626 at 3185.924 kb on - strand, within Psyr_2626 at 3186.268 kb on + strand, within Psyr_2626 at 3186.268 kb on + strand, within Psyr_2626 at 3186.269 kb on - strand, within Psyr_2626 at 3186.272 kb on - strand, within Psyr_2626 at 3186.272 kb on - strand, within Psyr_2626 at 3186.272 kb on - strand, within Psyr_2626 at 3186.272 kb on - strand, within Psyr_2626
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 3,183,904 + Psyr_2623 0.72 -1.9 3,183,904 + Psyr_2623 0.72 -0.8 3,183,904 + Psyr_2623 0.72 +0.7 3,183,905 - Psyr_2623 0.72 -2.4 3,183,906 + Psyr_2623 0.72 -1.4 3,183,907 - Psyr_2623 0.73 +1.2 3,183,949 + Psyr_2623 0.76 -0.8 3,183,968 - Psyr_2623 0.77 -1.9 3,184,298 + +0.6 3,184,302 + -1.1 3,184,303 - +0.4 3,184,327 - +0.2 3,184,327 - +0.3 3,184,327 - +1.4 3,184,327 - +0.8 3,184,597 + Psyr_2624 0.58 -0.1 3,184,799 + -0.6 3,184,802 + -0.7 3,184,803 - +0.2 3,184,848 - +0.0 3,184,938 + Psyr_2625 0.17 -0.4 3,184,970 + Psyr_2625 0.21 -2.0 3,184,970 + Psyr_2625 0.21 +1.2 3,184,979 - Psyr_2625 0.22 -0.6 3,185,007 + Psyr_2625 0.26 -1.4 3,185,144 + Psyr_2625 0.44 +0.5 3,185,144 + Psyr_2625 0.44 -0.8 3,185,145 - Psyr_2625 0.45 -0.3 3,185,145 - Psyr_2625 0.45 +0.0 3,185,145 - Psyr_2625 0.45 -0.8 3,185,148 + Psyr_2625 0.45 -0.5 3,185,149 - Psyr_2625 0.45 -1.0 3,185,179 + Psyr_2625 0.49 -0.7 3,185,222 + Psyr_2625 0.55 -1.9 3,185,222 + Psyr_2625 0.55 -0.3 3,185,223 - Psyr_2625 0.55 -1.6 3,185,243 + Psyr_2625 0.58 -1.2 3,185,243 + Psyr_2625 0.58 -0.8 3,185,243 + Psyr_2625 0.58 -3.4 3,185,244 - Psyr_2625 0.58 +0.4 3,185,246 + Psyr_2625 0.58 +0.2 3,185,246 + Psyr_2625 0.58 -0.3 3,185,249 + Psyr_2625 0.59 -2.1 3,185,249 + Psyr_2625 0.59 -0.8 3,185,249 + Psyr_2625 0.59 -1.2 3,185,249 + Psyr_2625 0.59 -2.2 3,185,249 + Psyr_2625 0.59 +0.4 3,185,250 - Psyr_2625 0.59 +2.0 3,185,250 - Psyr_2625 0.59 -0.8 3,185,343 + Psyr_2625 0.71 -0.5 3,185,347 + Psyr_2625 0.72 -1.1 3,185,542 + -1.1 3,185,543 - +0.8 3,185,543 - +0.1 3,185,543 - +1.2 3,185,543 - +1.0 3,185,543 - -1.4 3,185,543 - -1.8 3,185,587 + -0.7 3,185,601 - -1.8 3,185,628 + +0.5 3,185,629 - -1.4 3,185,781 + Psyr_2626 0.13 +0.4 3,185,797 + Psyr_2626 0.14 +0.3 3,185,849 + Psyr_2626 0.17 -0.0 3,185,899 + Psyr_2626 0.20 +0.0 3,185,923 + Psyr_2626 0.22 -2.1 3,185,923 + Psyr_2626 0.22 -0.6 3,185,923 + Psyr_2626 0.22 -0.4 3,185,923 + Psyr_2626 0.22 -1.4 3,185,924 - Psyr_2626 0.22 -1.8 3,186,268 + Psyr_2626 0.44 -0.0 3,186,268 + Psyr_2626 0.44 +1.2 3,186,269 - Psyr_2626 0.44 -1.0 3,186,272 - Psyr_2626 0.44 -0.2 3,186,272 - Psyr_2626 0.44 +0.9 3,186,272 - Psyr_2626 0.44 +0.5 3,186,272 - Psyr_2626 0.44 -1.2
Or see this region's nucleotide sequence