Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2526 and Psyr_2527 are separated by 93 nucleotides Psyr_2527 and Psyr_2528 overlap by 4 nucleotides Psyr_2528 and Psyr_2529 are separated by 20 nucleotides
Psyr_2526: Psyr_2526 - Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)-like protein, at 2,936,598 to 2,938,184
_2526
Psyr_2527: Psyr_2527 - GCN5-related N-acetyltransferase, at 2,938,278 to 2,939,426
_2527
Psyr_2528: Psyr_2528 - Polysaccharide deacetylase, at 2,939,423 to 2,940,325
_2528
Psyr_2529: Psyr_2529 - Amidohydrolase, at 2,940,346 to 2,941,605
_2529
Position (kb)
2938
2939
2940 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2937.307 kb on + strand, within Psyr_2526 at 2937.308 kb on - strand, within Psyr_2526 at 2937.373 kb on + strand, within Psyr_2526 at 2937.374 kb on - strand, within Psyr_2526 at 2937.626 kb on + strand, within Psyr_2526 at 2937.626 kb on + strand, within Psyr_2526 at 2937.736 kb on + strand, within Psyr_2526 at 2937.736 kb on + strand, within Psyr_2526 at 2937.736 kb on + strand, within Psyr_2526 at 2937.737 kb on - strand, within Psyr_2526 at 2937.917 kb on - strand, within Psyr_2526 at 2937.931 kb on + strand, within Psyr_2526 at 2937.932 kb on - strand, within Psyr_2526 at 2937.937 kb on + strand, within Psyr_2526 at 2937.937 kb on + strand, within Psyr_2526 at 2937.938 kb on - strand, within Psyr_2526 at 2938.003 kb on + strand, within Psyr_2526 at 2938.039 kb on + strand at 2938.039 kb on + strand at 2938.040 kb on - strand at 2938.040 kb on - strand at 2938.070 kb on + strand at 2938.152 kb on + strand at 2938.152 kb on + strand at 2938.152 kb on + strand at 2938.221 kb on + strand at 2938.221 kb on + strand at 2938.221 kb on + strand at 2938.266 kb on - strand at 2938.286 kb on + strand at 2938.286 kb on + strand at 2938.286 kb on + strand at 2938.286 kb on + strand at 2938.286 kb on + strand at 2938.491 kb on + strand, within Psyr_2527 at 2938.588 kb on - strand, within Psyr_2527 at 2938.636 kb on - strand, within Psyr_2527 at 2938.677 kb on + strand, within Psyr_2527 at 2938.678 kb on - strand, within Psyr_2527 at 2938.678 kb on - strand, within Psyr_2527 at 2938.681 kb on - strand, within Psyr_2527 at 2938.681 kb on - strand, within Psyr_2527 at 2938.707 kb on + strand, within Psyr_2527 at 2939.285 kb on + strand, within Psyr_2527 at 2939.285 kb on + strand, within Psyr_2527 at 2939.285 kb on + strand, within Psyr_2527 at 2939.286 kb on - strand, within Psyr_2527 at 2939.286 kb on - strand, within Psyr_2527 at 2939.286 kb on - strand, within Psyr_2527 at 2939.428 kb on + strand at 2939.428 kb on + strand at 2939.428 kb on + strand at 2939.429 kb on - strand at 2939.429 kb on - strand at 2939.429 kb on - strand at 2939.595 kb on - strand, within Psyr_2528 at 2939.595 kb on - strand, within Psyr_2528 at 2939.595 kb on - strand, within Psyr_2528 at 2939.595 kb on - strand, within Psyr_2528 at 2939.595 kb on - strand, within Psyr_2528 at 2939.987 kb on + strand, within Psyr_2528 at 2939.987 kb on + strand, within Psyr_2528 at 2939.989 kb on + strand, within Psyr_2528 at 2939.990 kb on - strand, within Psyr_2528 at 2939.990 kb on - strand, within Psyr_2528 at 2940.209 kb on + strand, within Psyr_2528 at 2940.210 kb on - strand, within Psyr_2528
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 2,937,307 + Psyr_2526 0.45 +0.8 2,937,308 - Psyr_2526 0.45 -0.1 2,937,373 + Psyr_2526 0.49 +0.4 2,937,374 - Psyr_2526 0.49 -0.0 2,937,626 + Psyr_2526 0.65 -1.7 2,937,626 + Psyr_2526 0.65 +0.9 2,937,736 + Psyr_2526 0.72 -0.1 2,937,736 + Psyr_2526 0.72 +1.0 2,937,736 + Psyr_2526 0.72 -0.5 2,937,737 - Psyr_2526 0.72 +4.0 2,937,917 - Psyr_2526 0.83 -1.5 2,937,931 + Psyr_2526 0.84 -1.0 2,937,932 - Psyr_2526 0.84 +1.0 2,937,937 + Psyr_2526 0.84 -0.0 2,937,937 + Psyr_2526 0.84 -0.1 2,937,938 - Psyr_2526 0.84 -0.7 2,938,003 + Psyr_2526 0.89 +0.3 2,938,039 + -0.5 2,938,039 + -2.5 2,938,040 - -0.2 2,938,040 - -0.8 2,938,070 + +1.7 2,938,152 + -0.2 2,938,152 + -0.8 2,938,152 + -0.2 2,938,221 + +1.3 2,938,221 + -0.8 2,938,221 + -0.1 2,938,266 - +0.5 2,938,286 + -1.4 2,938,286 + +0.4 2,938,286 + -1.6 2,938,286 + -0.4 2,938,286 + +0.7 2,938,491 + Psyr_2527 0.19 -1.4 2,938,588 - Psyr_2527 0.27 +0.2 2,938,636 - Psyr_2527 0.31 +0.1 2,938,677 + Psyr_2527 0.35 -0.4 2,938,678 - Psyr_2527 0.35 -1.0 2,938,678 - Psyr_2527 0.35 +1.2 2,938,681 - Psyr_2527 0.35 -0.2 2,938,681 - Psyr_2527 0.35 -0.9 2,938,707 + Psyr_2527 0.37 +0.1 2,939,285 + Psyr_2527 0.88 -1.1 2,939,285 + Psyr_2527 0.88 -0.8 2,939,285 + Psyr_2527 0.88 -0.6 2,939,286 - Psyr_2527 0.88 -2.2 2,939,286 - Psyr_2527 0.88 -1.4 2,939,286 - Psyr_2527 0.88 -2.8 2,939,428 + +1.2 2,939,428 + -1.6 2,939,428 + +0.2 2,939,429 - +0.1 2,939,429 - +1.2 2,939,429 - +0.8 2,939,595 - Psyr_2528 0.19 +0.5 2,939,595 - Psyr_2528 0.19 +0.5 2,939,595 - Psyr_2528 0.19 +0.2 2,939,595 - Psyr_2528 0.19 +0.2 2,939,595 - Psyr_2528 0.19 -1.8 2,939,987 + Psyr_2528 0.62 -1.2 2,939,987 + Psyr_2528 0.62 +0.1 2,939,989 + Psyr_2528 0.63 +0.6 2,939,990 - Psyr_2528 0.63 +0.5 2,939,990 - Psyr_2528 0.63 +1.2 2,940,209 + Psyr_2528 0.87 -1.2 2,940,210 - Psyr_2528 0.87 +2.5
Or see this region's nucleotide sequence