Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2517 and Psyr_2518 are separated by 293 nucleotides Psyr_2518 and Psyr_2519 are separated by 19 nucleotides Psyr_2519 and Psyr_2520 are separated by 204 nucleotides Psyr_2520 and Psyr_2521 overlap by 4 nucleotides
Psyr_2517: Psyr_2517 - hypothetical protein, at 2,926,540 to 2,926,785
_2517
Psyr_2518: Psyr_2518 - conserved hypothetical protein, at 2,927,079 to 2,927,765
_2518
Psyr_2519: Psyr_2519 - Aldo/keto reductase, at 2,927,785 to 2,928,780
_2519
Psyr_2520: Psyr_2520 - SOS-response transcriptional repressor, LexA, at 2,928,985 to 2,929,602
_2520
Psyr_2521: Psyr_2521 - conserved hypothetical protein, at 2,929,599 to 2,930,222
_2521
Position (kb)
2927
2928
2929 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2926.789 kb on + strand at 2926.812 kb on - strand at 2926.954 kb on + strand at 2927.022 kb on + strand at 2927.084 kb on + strand at 2927.084 kb on + strand at 2927.231 kb on - strand, within Psyr_2518 at 2927.231 kb on - strand, within Psyr_2518 at 2927.403 kb on + strand, within Psyr_2518 at 2927.403 kb on + strand, within Psyr_2518 at 2927.403 kb on + strand, within Psyr_2518 at 2927.409 kb on + strand, within Psyr_2518 at 2927.409 kb on + strand, within Psyr_2518 at 2927.409 kb on + strand, within Psyr_2518 at 2927.409 kb on + strand, within Psyr_2518 at 2927.410 kb on - strand, within Psyr_2518 at 2927.410 kb on - strand, within Psyr_2518 at 2927.410 kb on - strand, within Psyr_2518 at 2927.410 kb on - strand, within Psyr_2518 at 2927.526 kb on + strand, within Psyr_2518 at 2927.526 kb on + strand, within Psyr_2518 at 2927.527 kb on - strand, within Psyr_2518 at 2927.527 kb on - strand, within Psyr_2518 at 2927.544 kb on + strand, within Psyr_2518 at 2927.544 kb on + strand, within Psyr_2518 at 2927.544 kb on + strand, within Psyr_2518 at 2927.544 kb on + strand, within Psyr_2518 at 2927.544 kb on + strand, within Psyr_2518 at 2927.544 kb on + strand, within Psyr_2518 at 2927.544 kb on + strand, within Psyr_2518 at 2927.544 kb on + strand, within Psyr_2518 at 2927.544 kb on + strand, within Psyr_2518 at 2927.707 kb on - strand at 2927.819 kb on + strand at 2927.820 kb on - strand at 2927.925 kb on - strand, within Psyr_2519 at 2928.170 kb on + strand, within Psyr_2519 at 2928.325 kb on + strand, within Psyr_2519 at 2928.325 kb on + strand, within Psyr_2519 at 2928.325 kb on + strand, within Psyr_2519 at 2928.325 kb on + strand, within Psyr_2519 at 2928.325 kb on + strand, within Psyr_2519 at 2928.326 kb on - strand, within Psyr_2519 at 2928.326 kb on - strand, within Psyr_2519 at 2928.326 kb on - strand, within Psyr_2519 at 2928.416 kb on + strand, within Psyr_2519 at 2928.416 kb on + strand, within Psyr_2519 at 2928.417 kb on - strand, within Psyr_2519 at 2928.417 kb on - strand, within Psyr_2519 at 2928.417 kb on - strand, within Psyr_2519 at 2928.548 kb on + strand, within Psyr_2519 at 2928.549 kb on - strand, within Psyr_2519 at 2928.549 kb on - strand, within Psyr_2519 at 2928.553 kb on - strand, within Psyr_2519 at 2928.553 kb on - strand, within Psyr_2519 at 2928.611 kb on + strand, within Psyr_2519 at 2928.611 kb on + strand, within Psyr_2519 at 2928.612 kb on - strand, within Psyr_2519 at 2928.612 kb on - strand, within Psyr_2519 at 2928.612 kb on - strand, within Psyr_2519 at 2928.701 kb on + strand at 2928.701 kb on + strand at 2928.702 kb on - strand at 2928.702 kb on - strand at 2928.702 kb on - strand at 2928.702 kb on - strand at 2928.892 kb on + strand at 2928.969 kb on - strand at 2929.004 kb on + strand at 2929.005 kb on - strand at 2929.029 kb on + strand at 2929.030 kb on - strand at 2929.336 kb on + strand, within Psyr_2520 at 2929.410 kb on + strand, within Psyr_2520
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 2,926,789 + -0.7 2,926,812 - -3.8 2,926,954 + +2.8 2,927,022 + -0.9 2,927,084 + -0.8 2,927,084 + -2.4 2,927,231 - Psyr_2518 0.22 +0.5 2,927,231 - Psyr_2518 0.22 +0.7 2,927,403 + Psyr_2518 0.47 -2.0 2,927,403 + Psyr_2518 0.47 +0.1 2,927,403 + Psyr_2518 0.47 -0.4 2,927,409 + Psyr_2518 0.48 -2.1 2,927,409 + Psyr_2518 0.48 +0.6 2,927,409 + Psyr_2518 0.48 +0.8 2,927,409 + Psyr_2518 0.48 -0.4 2,927,410 - Psyr_2518 0.48 +0.4 2,927,410 - Psyr_2518 0.48 -0.8 2,927,410 - Psyr_2518 0.48 -0.0 2,927,410 - Psyr_2518 0.48 -1.0 2,927,526 + Psyr_2518 0.65 +1.2 2,927,526 + Psyr_2518 0.65 -2.5 2,927,527 - Psyr_2518 0.65 -0.4 2,927,527 - Psyr_2518 0.65 -0.2 2,927,544 + Psyr_2518 0.68 +0.5 2,927,544 + Psyr_2518 0.68 +0.6 2,927,544 + Psyr_2518 0.68 +0.9 2,927,544 + Psyr_2518 0.68 +2.8 2,927,544 + Psyr_2518 0.68 -2.0 2,927,544 + Psyr_2518 0.68 -0.5 2,927,544 + Psyr_2518 0.68 -2.4 2,927,544 + Psyr_2518 0.68 -0.4 2,927,544 + Psyr_2518 0.68 -0.2 2,927,707 - +2.0 2,927,819 + +1.0 2,927,820 - +0.8 2,927,925 - Psyr_2519 0.14 -0.6 2,928,170 + Psyr_2519 0.39 +0.3 2,928,325 + Psyr_2519 0.54 +0.9 2,928,325 + Psyr_2519 0.54 +0.3 2,928,325 + Psyr_2519 0.54 +0.5 2,928,325 + Psyr_2519 0.54 +0.2 2,928,325 + Psyr_2519 0.54 +1.3 2,928,326 - Psyr_2519 0.54 +0.3 2,928,326 - Psyr_2519 0.54 +0.1 2,928,326 - Psyr_2519 0.54 +0.5 2,928,416 + Psyr_2519 0.63 +0.7 2,928,416 + Psyr_2519 0.63 -0.7 2,928,417 - Psyr_2519 0.63 +3.2 2,928,417 - Psyr_2519 0.63 +0.4 2,928,417 - Psyr_2519 0.63 +1.8 2,928,548 + Psyr_2519 0.77 -0.2 2,928,549 - Psyr_2519 0.77 +0.5 2,928,549 - Psyr_2519 0.77 +2.2 2,928,553 - Psyr_2519 0.77 -0.6 2,928,553 - Psyr_2519 0.77 +0.2 2,928,611 + Psyr_2519 0.83 -1.6 2,928,611 + Psyr_2519 0.83 -2.7 2,928,612 - Psyr_2519 0.83 +1.5 2,928,612 - Psyr_2519 0.83 +0.9 2,928,612 - Psyr_2519 0.83 +0.7 2,928,701 + -0.3 2,928,701 + -1.2 2,928,702 - -0.3 2,928,702 - -0.6 2,928,702 - -0.8 2,928,702 - -0.6 2,928,892 + -0.4 2,928,969 - +0.2 2,929,004 + -1.4 2,929,005 - +0.7 2,929,029 + -0.4 2,929,030 - -0.4 2,929,336 + Psyr_2520 0.57 +0.1 2,929,410 + Psyr_2520 0.69 -0.6
Or see this region's nucleotide sequence