Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2458 and Psyr_2459 are separated by 55 nucleotides Psyr_2459 and Psyr_2460 are separated by 205 nucleotides Psyr_2460 and Psyr_2461 are separated by 164 nucleotides Psyr_2461 and Psyr_2462 overlap by 17 nucleotides
Psyr_2458: Psyr_2458 - Sterol-binding protein, at 2,846,627 to 2,846,944
_2458
Psyr_2459: Psyr_2459 - Phosphoglycerate/bisphosphoglycerate mutase, at 2,847,000 to 2,847,710
_2459
Psyr_2460: Psyr_2460 - inner membrane peptidase, Serine peptidase, MEROPS family S49, at 2,847,916 to 2,848,938
_2460
Psyr_2461: Psyr_2461 - Uncharacterized conserved protein UCP030820, at 2,849,103 to 2,849,597
_2461
Psyr_2462: Psyr_2462 - Nitrite/sulfite reductase, hemoprotein beta-component, ferrodoxin-like:Nitrite and sulphite reductase 4Fe-4S region, at 2,849,581 to 2,851,239
_2462
Position (kb)
2847
2848
2849 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2846.921 kb on + strand at 2846.933 kb on + strand at 2846.933 kb on + strand at 2846.959 kb on - strand at 2846.963 kb on + strand at 2846.963 kb on + strand at 2846.964 kb on - strand at 2846.964 kb on - strand at 2846.964 kb on - strand at 2846.964 kb on - strand at 2846.964 kb on - strand at 2847.008 kb on - strand at 2847.092 kb on + strand, within Psyr_2459 at 2847.093 kb on - strand, within Psyr_2459 at 2847.110 kb on + strand, within Psyr_2459 at 2847.110 kb on + strand, within Psyr_2459 at 2847.111 kb on - strand, within Psyr_2459 at 2847.111 kb on - strand, within Psyr_2459 at 2847.302 kb on + strand, within Psyr_2459 at 2847.387 kb on + strand, within Psyr_2459 at 2847.387 kb on + strand, within Psyr_2459 at 2847.387 kb on + strand, within Psyr_2459 at 2847.387 kb on + strand, within Psyr_2459 at 2847.387 kb on + strand, within Psyr_2459 at 2847.388 kb on - strand, within Psyr_2459 at 2847.388 kb on - strand, within Psyr_2459 at 2847.388 kb on - strand, within Psyr_2459 at 2847.388 kb on - strand, within Psyr_2459 at 2847.390 kb on - strand, within Psyr_2459 at 2847.390 kb on - strand, within Psyr_2459 at 2847.515 kb on - strand, within Psyr_2459 at 2847.515 kb on - strand, within Psyr_2459 at 2847.525 kb on + strand, within Psyr_2459 at 2847.526 kb on - strand, within Psyr_2459 at 2847.526 kb on - strand, within Psyr_2459 at 2847.526 kb on - strand, within Psyr_2459 at 2847.526 kb on - strand, within Psyr_2459 at 2847.526 kb on - strand, within Psyr_2459 at 2847.655 kb on + strand at 2847.656 kb on - strand at 2847.690 kb on + strand at 2847.697 kb on + strand at 2847.698 kb on - strand at 2847.698 kb on - strand at 2847.698 kb on - strand at 2847.698 kb on - strand at 2847.786 kb on - strand at 2847.858 kb on - strand at 2847.911 kb on + strand at 2848.069 kb on + strand, within Psyr_2460 at 2848.069 kb on + strand, within Psyr_2460 at 2848.069 kb on + strand, within Psyr_2460 at 2848.069 kb on + strand, within Psyr_2460 at 2848.070 kb on - strand, within Psyr_2460 at 2848.070 kb on - strand, within Psyr_2460 at 2848.195 kb on + strand, within Psyr_2460 at 2848.195 kb on + strand, within Psyr_2460 at 2848.395 kb on - strand, within Psyr_2460 at 2848.426 kb on + strand, within Psyr_2460 at 2848.426 kb on + strand, within Psyr_2460 at 2848.426 kb on + strand, within Psyr_2460 at 2848.426 kb on + strand, within Psyr_2460 at 2848.426 kb on + strand, within Psyr_2460 at 2848.427 kb on - strand, within Psyr_2460 at 2848.427 kb on - strand, within Psyr_2460 at 2848.570 kb on + strand, within Psyr_2460 at 2848.570 kb on + strand, within Psyr_2460 at 2848.571 kb on - strand, within Psyr_2460 at 2848.571 kb on - strand, within Psyr_2460 at 2848.571 kb on - strand, within Psyr_2460 at 2848.721 kb on + strand, within Psyr_2460 at 2848.721 kb on + strand, within Psyr_2460 at 2848.721 kb on + strand, within Psyr_2460 at 2848.722 kb on - strand, within Psyr_2460 at 2848.722 kb on - strand, within Psyr_2460 at 2848.828 kb on + strand, within Psyr_2460 at 2848.828 kb on + strand, within Psyr_2460 at 2848.829 kb on - strand, within Psyr_2460 at 2848.939 kb on + strand at 2848.939 kb on + strand at 2848.939 kb on + strand at 2848.939 kb on + strand at 2848.939 kb on + strand at 2848.939 kb on + strand at 2848.940 kb on - strand at 2848.940 kb on - strand at 2848.945 kb on + strand at 2848.945 kb on + strand at 2848.945 kb on + strand at 2848.945 kb on + strand at 2848.945 kb on + strand at 2848.946 kb on - strand at 2848.946 kb on - strand at 2849.149 kb on + strand at 2849.188 kb on + strand, within Psyr_2461 at 2849.242 kb on + strand, within Psyr_2461 at 2849.242 kb on + strand, within Psyr_2461 at 2849.243 kb on - strand, within Psyr_2461 at 2849.243 kb on - strand, within Psyr_2461 at 2849.294 kb on - strand, within Psyr_2461 at 2849.597 kb on + strand at 2849.628 kb on + strand at 2849.629 kb on - strand at 2849.636 kb on + strand at 2849.637 kb on - strand at 2849.637 kb on - strand at 2849.679 kb on - strand at 2849.792 kb on + strand, within Psyr_2462 at 2849.877 kb on + strand, within Psyr_2462 at 2849.878 kb on - strand, within Psyr_2462 at 2849.880 kb on + strand, within Psyr_2462
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 2,846,921 + +0.8 2,846,933 + -0.0 2,846,933 + +0.5 2,846,959 - -2.7 2,846,963 + +1.6 2,846,963 + -0.7 2,846,964 - -1.0 2,846,964 - -0.5 2,846,964 - +1.1 2,846,964 - -0.9 2,846,964 - +0.4 2,847,008 - +0.8 2,847,092 + Psyr_2459 0.13 +1.1 2,847,093 - Psyr_2459 0.13 -0.5 2,847,110 + Psyr_2459 0.15 +1.7 2,847,110 + Psyr_2459 0.15 +2.1 2,847,111 - Psyr_2459 0.16 +0.1 2,847,111 - Psyr_2459 0.16 +0.6 2,847,302 + Psyr_2459 0.42 -1.7 2,847,387 + Psyr_2459 0.54 -0.5 2,847,387 + Psyr_2459 0.54 +0.5 2,847,387 + Psyr_2459 0.54 -0.6 2,847,387 + Psyr_2459 0.54 +2.0 2,847,387 + Psyr_2459 0.54 +0.4 2,847,388 - Psyr_2459 0.55 +0.6 2,847,388 - Psyr_2459 0.55 -1.2 2,847,388 - Psyr_2459 0.55 +0.5 2,847,388 - Psyr_2459 0.55 +0.6 2,847,390 - Psyr_2459 0.55 +0.3 2,847,390 - Psyr_2459 0.55 +0.2 2,847,515 - Psyr_2459 0.72 -2.3 2,847,515 - Psyr_2459 0.72 +2.1 2,847,525 + Psyr_2459 0.74 +0.8 2,847,526 - Psyr_2459 0.74 -0.2 2,847,526 - Psyr_2459 0.74 -1.2 2,847,526 - Psyr_2459 0.74 -1.9 2,847,526 - Psyr_2459 0.74 +1.9 2,847,526 - Psyr_2459 0.74 +0.6 2,847,655 + -1.2 2,847,656 - -0.8 2,847,690 + -2.2 2,847,697 + +1.0 2,847,698 - +0.0 2,847,698 - -0.2 2,847,698 - -1.2 2,847,698 - -1.7 2,847,786 - +1.9 2,847,858 - +1.5 2,847,911 + -0.5 2,848,069 + Psyr_2460 0.15 -2.8 2,848,069 + Psyr_2460 0.15 -1.7 2,848,069 + Psyr_2460 0.15 +2.1 2,848,069 + Psyr_2460 0.15 -0.7 2,848,070 - Psyr_2460 0.15 -2.0 2,848,070 - Psyr_2460 0.15 -0.5 2,848,195 + Psyr_2460 0.27 -0.9 2,848,195 + Psyr_2460 0.27 -0.9 2,848,395 - Psyr_2460 0.47 -2.2 2,848,426 + Psyr_2460 0.50 -1.3 2,848,426 + Psyr_2460 0.50 -3.2 2,848,426 + Psyr_2460 0.50 -2.5 2,848,426 + Psyr_2460 0.50 -2.0 2,848,426 + Psyr_2460 0.50 -0.6 2,848,427 - Psyr_2460 0.50 -2.2 2,848,427 - Psyr_2460 0.50 -0.7 2,848,570 + Psyr_2460 0.64 -2.7 2,848,570 + Psyr_2460 0.64 -1.7 2,848,571 - Psyr_2460 0.64 -2.6 2,848,571 - Psyr_2460 0.64 -1.7 2,848,571 - Psyr_2460 0.64 -0.2 2,848,721 + Psyr_2460 0.79 +1.1 2,848,721 + Psyr_2460 0.79 -1.6 2,848,721 + Psyr_2460 0.79 +1.4 2,848,722 - Psyr_2460 0.79 -0.5 2,848,722 - Psyr_2460 0.79 -1.5 2,848,828 + Psyr_2460 0.89 -1.3 2,848,828 + Psyr_2460 0.89 -1.5 2,848,829 - Psyr_2460 0.89 -0.9 2,848,939 + +0.5 2,848,939 + +1.1 2,848,939 + -3.5 2,848,939 + +0.4 2,848,939 + -0.2 2,848,939 + -1.2 2,848,940 - -1.2 2,848,940 - +1.1 2,848,945 + -0.2 2,848,945 + -1.1 2,848,945 + -0.0 2,848,945 + +1.1 2,848,945 + -0.5 2,848,946 - +0.2 2,848,946 - +0.2 2,849,149 + -2.3 2,849,188 + Psyr_2461 0.17 +0.4 2,849,242 + Psyr_2461 0.28 +0.6 2,849,242 + Psyr_2461 0.28 -2.0 2,849,243 - Psyr_2461 0.28 +0.1 2,849,243 - Psyr_2461 0.28 -0.3 2,849,294 - Psyr_2461 0.39 +1.0 2,849,597 + -0.9 2,849,628 + +0.6 2,849,629 - -0.1 2,849,636 + +0.3 2,849,637 - -0.8 2,849,637 - +1.0 2,849,679 - +1.1 2,849,792 + Psyr_2462 0.13 +0.5 2,849,877 + Psyr_2462 0.18 +0.2 2,849,878 - Psyr_2462 0.18 +0.1 2,849,880 + Psyr_2462 0.18 -2.9
Or see this region's nucleotide sequence