Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2439 and Psyr_2440 are separated by 87 nucleotides Psyr_2440 and Psyr_2441 are separated by 240 nucleotides
Psyr_2439: Psyr_2439 - mannitol ABC transporter membrane protein / sorbitol ABC transporter membrane protein, at 2,823,940 to 2,824,872
_2439
Psyr_2440: Psyr_2440 - mannitol-binding protein / sorbitol-binding protein, at 2,824,960 to 2,826,282
_2440
Psyr_2441: Psyr_2441 - transcriptional regulator, AraC family, at 2,826,523 to 2,827,446
_2441
Position (kb)
2824
2825
2826
2827 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2824.004 kb on - strand at 2824.070 kb on + strand, within Psyr_2439 at 2824.070 kb on + strand, within Psyr_2439 at 2824.070 kb on + strand, within Psyr_2439 at 2824.070 kb on + strand, within Psyr_2439 at 2824.070 kb on + strand, within Psyr_2439 at 2824.070 kb on + strand, within Psyr_2439 at 2824.071 kb on - strand, within Psyr_2439 at 2824.071 kb on - strand, within Psyr_2439 at 2824.071 kb on - strand, within Psyr_2439 at 2824.071 kb on - strand, within Psyr_2439 at 2824.071 kb on - strand, within Psyr_2439 at 2824.703 kb on + strand, within Psyr_2439 at 2824.721 kb on + strand, within Psyr_2439 at 2824.721 kb on + strand, within Psyr_2439 at 2824.721 kb on + strand, within Psyr_2439 at 2824.721 kb on + strand, within Psyr_2439 at 2824.722 kb on - strand, within Psyr_2439 at 2824.722 kb on - strand, within Psyr_2439 at 2824.866 kb on + strand at 2824.866 kb on + strand at 2824.962 kb on - strand at 2825.047 kb on + strand at 2825.048 kb on - strand at 2825.048 kb on - strand at 2825.048 kb on - strand at 2825.048 kb on - strand at 2825.048 kb on - strand at 2825.130 kb on - strand, within Psyr_2440 at 2825.130 kb on - strand, within Psyr_2440 at 2825.130 kb on - strand, within Psyr_2440 at 2825.130 kb on - strand, within Psyr_2440 at 2825.217 kb on - strand, within Psyr_2440 at 2825.228 kb on + strand, within Psyr_2440 at 2825.228 kb on + strand, within Psyr_2440 at 2825.229 kb on - strand, within Psyr_2440 at 2825.229 kb on - strand, within Psyr_2440 at 2825.229 kb on - strand, within Psyr_2440 at 2825.229 kb on - strand, within Psyr_2440 at 2825.229 kb on - strand, within Psyr_2440 at 2825.229 kb on - strand, within Psyr_2440 at 2825.378 kb on + strand, within Psyr_2440 at 2825.421 kb on - strand, within Psyr_2440 at 2825.421 kb on - strand, within Psyr_2440 at 2825.421 kb on - strand, within Psyr_2440 at 2825.421 kb on - strand, within Psyr_2440 at 2825.587 kb on - strand, within Psyr_2440 at 2825.595 kb on - strand, within Psyr_2440 at 2825.595 kb on - strand, within Psyr_2440 at 2825.595 kb on - strand, within Psyr_2440 at 2825.669 kb on + strand, within Psyr_2440 at 2825.741 kb on + strand, within Psyr_2440 at 2825.799 kb on + strand, within Psyr_2440 at 2825.852 kb on + strand, within Psyr_2440 at 2825.852 kb on + strand, within Psyr_2440 at 2825.852 kb on + strand, within Psyr_2440 at 2825.852 kb on + strand, within Psyr_2440 at 2825.853 kb on - strand, within Psyr_2440 at 2825.874 kb on - strand, within Psyr_2440 at 2825.874 kb on - strand, within Psyr_2440 at 2825.874 kb on - strand, within Psyr_2440 at 2825.921 kb on - strand, within Psyr_2440 at 2825.948 kb on + strand, within Psyr_2440 at 2825.948 kb on + strand, within Psyr_2440 at 2825.949 kb on - strand, within Psyr_2440 at 2825.949 kb on - strand, within Psyr_2440 at 2826.011 kb on + strand, within Psyr_2440 at 2826.011 kb on + strand, within Psyr_2440 at 2826.011 kb on + strand, within Psyr_2440 at 2826.011 kb on + strand, within Psyr_2440 at 2826.011 kb on + strand, within Psyr_2440 at 2826.011 kb on + strand, within Psyr_2440 at 2826.011 kb on + strand, within Psyr_2440 at 2826.011 kb on + strand, within Psyr_2440 at 2826.012 kb on - strand, within Psyr_2440 at 2826.012 kb on - strand, within Psyr_2440 at 2826.012 kb on - strand, within Psyr_2440 at 2826.012 kb on - strand, within Psyr_2440 at 2826.012 kb on - strand, within Psyr_2440 at 2826.012 kb on - strand, within Psyr_2440 at 2826.012 kb on - strand, within Psyr_2440 at 2826.326 kb on + strand at 2826.370 kb on + strand at 2826.370 kb on + strand at 2826.371 kb on - strand at 2826.625 kb on - strand, within Psyr_2441 at 2826.703 kb on - strand, within Psyr_2441 at 2826.767 kb on + strand, within Psyr_2441 at 2826.768 kb on - strand, within Psyr_2441 at 2826.837 kb on + strand, within Psyr_2441 at 2826.838 kb on - strand, within Psyr_2441 at 2827.194 kb on + strand, within Psyr_2441 at 2827.194 kb on + strand, within Psyr_2441 at 2827.194 kb on + strand, within Psyr_2441 at 2827.250 kb on + strand, within Psyr_2441 at 2827.250 kb on + strand, within Psyr_2441
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 2,824,004 - -1.2 2,824,070 + Psyr_2439 0.14 -2.8 2,824,070 + Psyr_2439 0.14 +2.5 2,824,070 + Psyr_2439 0.14 -1.5 2,824,070 + Psyr_2439 0.14 +0.7 2,824,070 + Psyr_2439 0.14 -1.1 2,824,070 + Psyr_2439 0.14 +0.7 2,824,071 - Psyr_2439 0.14 -2.5 2,824,071 - Psyr_2439 0.14 +2.1 2,824,071 - Psyr_2439 0.14 +0.5 2,824,071 - Psyr_2439 0.14 +0.3 2,824,071 - Psyr_2439 0.14 +0.8 2,824,703 + Psyr_2439 0.82 -1.7 2,824,721 + Psyr_2439 0.84 +0.8 2,824,721 + Psyr_2439 0.84 +0.5 2,824,721 + Psyr_2439 0.84 +1.3 2,824,721 + Psyr_2439 0.84 +0.2 2,824,722 - Psyr_2439 0.84 -2.2 2,824,722 - Psyr_2439 0.84 -3.0 2,824,866 + +1.7 2,824,866 + -0.1 2,824,962 - -1.2 2,825,047 + +0.4 2,825,048 - -0.6 2,825,048 - -0.5 2,825,048 - +1.1 2,825,048 - +3.3 2,825,048 - +0.1 2,825,130 - Psyr_2440 0.13 +0.6 2,825,130 - Psyr_2440 0.13 -1.1 2,825,130 - Psyr_2440 0.13 +0.1 2,825,130 - Psyr_2440 0.13 +0.7 2,825,217 - Psyr_2440 0.19 +0.1 2,825,228 + Psyr_2440 0.20 -0.7 2,825,228 + Psyr_2440 0.20 -0.9 2,825,229 - Psyr_2440 0.20 +1.1 2,825,229 - Psyr_2440 0.20 +0.5 2,825,229 - Psyr_2440 0.20 -0.2 2,825,229 - Psyr_2440 0.20 +0.3 2,825,229 - Psyr_2440 0.20 +1.1 2,825,229 - Psyr_2440 0.20 +1.8 2,825,378 + Psyr_2440 0.32 -0.7 2,825,421 - Psyr_2440 0.35 -1.5 2,825,421 - Psyr_2440 0.35 -0.4 2,825,421 - Psyr_2440 0.35 -1.0 2,825,421 - Psyr_2440 0.35 -0.6 2,825,587 - Psyr_2440 0.47 +0.1 2,825,595 - Psyr_2440 0.48 -1.2 2,825,595 - Psyr_2440 0.48 -0.2 2,825,595 - Psyr_2440 0.48 -0.6 2,825,669 + Psyr_2440 0.54 -1.3 2,825,741 + Psyr_2440 0.59 +0.6 2,825,799 + Psyr_2440 0.63 +0.5 2,825,852 + Psyr_2440 0.67 -0.4 2,825,852 + Psyr_2440 0.67 -1.5 2,825,852 + Psyr_2440 0.67 -0.2 2,825,852 + Psyr_2440 0.67 -1.2 2,825,853 - Psyr_2440 0.67 -0.7 2,825,874 - Psyr_2440 0.69 +0.5 2,825,874 - Psyr_2440 0.69 +0.4 2,825,874 - Psyr_2440 0.69 +0.5 2,825,921 - Psyr_2440 0.73 -1.1 2,825,948 + Psyr_2440 0.75 +1.3 2,825,948 + Psyr_2440 0.75 +0.6 2,825,949 - Psyr_2440 0.75 -1.2 2,825,949 - Psyr_2440 0.75 -2.6 2,826,011 + Psyr_2440 0.79 -0.5 2,826,011 + Psyr_2440 0.79 -0.4 2,826,011 + Psyr_2440 0.79 +0.2 2,826,011 + Psyr_2440 0.79 +0.6 2,826,011 + Psyr_2440 0.79 -0.5 2,826,011 + Psyr_2440 0.79 -2.2 2,826,011 + Psyr_2440 0.79 +0.0 2,826,011 + Psyr_2440 0.79 -0.3 2,826,012 - Psyr_2440 0.80 -1.5 2,826,012 - Psyr_2440 0.80 +0.5 2,826,012 - Psyr_2440 0.80 -1.4 2,826,012 - Psyr_2440 0.80 -0.9 2,826,012 - Psyr_2440 0.80 +1.1 2,826,012 - Psyr_2440 0.80 +0.8 2,826,012 - Psyr_2440 0.80 -0.5 2,826,326 + +2.4 2,826,370 + +0.7 2,826,370 + +0.1 2,826,371 - -0.6 2,826,625 - Psyr_2441 0.11 +1.1 2,826,703 - Psyr_2441 0.19 -1.6 2,826,767 + Psyr_2441 0.26 -0.2 2,826,768 - Psyr_2441 0.27 -1.1 2,826,837 + Psyr_2441 0.34 +1.3 2,826,838 - Psyr_2441 0.34 -2.5 2,827,194 + Psyr_2441 0.73 -2.9 2,827,194 + Psyr_2441 0.73 +0.3 2,827,194 + Psyr_2441 0.73 -1.0 2,827,250 + Psyr_2441 0.79 +0.8 2,827,250 + Psyr_2441 0.79 -1.5
Or see this region's nucleotide sequence