Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2336 and Psyr_2337 are separated by 45 nucleotides Psyr_2337 and Psyr_2338 are separated by 65 nucleotides Psyr_2338 and Psyr_2339 are separated by 50 nucleotides
Psyr_2336: Psyr_2336 - transcriptional regulator, IclR family, at 2,713,737 to 2,714,576
_2336
Psyr_2337: Psyr_2337 - conserved hypothetical protein, at 2,714,622 to 2,715,584
_2337
Psyr_2338: Psyr_2338 - Protein of unknown function DUF1275, at 2,715,650 to 2,716,354
_2338
Psyr_2339: Psyr_2339 - hypothetical protein, at 2,716,405 to 2,716,632
_2339
Position (kb)
2714
2715
2716 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2713.685 kb on - strand at 2713.702 kb on + strand at 2713.720 kb on + strand at 2713.721 kb on - strand at 2713.723 kb on - strand at 2713.730 kb on + strand at 2713.730 kb on + strand at 2713.730 kb on + strand at 2713.731 kb on - strand at 2713.731 kb on - strand at 2714.250 kb on + strand, within Psyr_2336 at 2714.863 kb on + strand, within Psyr_2337 at 2714.864 kb on - strand, within Psyr_2337 at 2715.230 kb on - strand, within Psyr_2337 at 2715.230 kb on - strand, within Psyr_2337 at 2715.307 kb on + strand, within Psyr_2337 at 2715.308 kb on - strand, within Psyr_2337 at 2715.613 kb on - strand at 2715.613 kb on - strand at 2715.623 kb on + strand at 2715.623 kb on + strand at 2715.623 kb on + strand at 2715.624 kb on - strand at 2715.663 kb on + strand at 2715.663 kb on + strand at 2715.663 kb on + strand at 2715.664 kb on - strand at 2715.664 kb on - strand at 2715.664 kb on - strand at 2715.664 kb on - strand at 2715.664 kb on - strand at 2715.776 kb on + strand, within Psyr_2338 at 2715.776 kb on + strand, within Psyr_2338 at 2715.776 kb on + strand, within Psyr_2338 at 2715.776 kb on + strand, within Psyr_2338 at 2715.777 kb on - strand, within Psyr_2338 at 2715.777 kb on - strand, within Psyr_2338 at 2715.777 kb on - strand, within Psyr_2338 at 2715.897 kb on + strand, within Psyr_2338 at 2715.897 kb on + strand, within Psyr_2338 at 2715.897 kb on + strand, within Psyr_2338 at 2715.897 kb on + strand, within Psyr_2338 at 2715.897 kb on + strand, within Psyr_2338 at 2715.897 kb on + strand, within Psyr_2338 at 2715.898 kb on - strand, within Psyr_2338 at 2715.898 kb on - strand, within Psyr_2338 at 2715.898 kb on - strand, within Psyr_2338 at 2715.898 kb on - strand, within Psyr_2338 at 2715.898 kb on - strand, within Psyr_2338 at 2715.986 kb on - strand, within Psyr_2338 at 2716.406 kb on + strand at 2716.407 kb on - strand at 2716.442 kb on - strand, within Psyr_2339 at 2716.442 kb on - strand, within Psyr_2339 at 2716.442 kb on - strand, within Psyr_2339 at 2716.454 kb on + strand, within Psyr_2339 at 2716.454 kb on + strand, within Psyr_2339 at 2716.481 kb on + strand, within Psyr_2339 at 2716.482 kb on - strand, within Psyr_2339
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 2,713,685 - +0.6 2,713,702 + -0.4 2,713,720 + +0.3 2,713,721 - +0.6 2,713,723 - -2.5 2,713,730 + +0.4 2,713,730 + -0.4 2,713,730 + +1.0 2,713,731 - -0.2 2,713,731 - +1.4 2,714,250 + Psyr_2336 0.61 +2.9 2,714,863 + Psyr_2337 0.25 -0.3 2,714,864 - Psyr_2337 0.25 -0.4 2,715,230 - Psyr_2337 0.63 -2.8 2,715,230 - Psyr_2337 0.63 -0.1 2,715,307 + Psyr_2337 0.71 +0.0 2,715,308 - Psyr_2337 0.71 -1.8 2,715,613 - +1.0 2,715,613 - -0.5 2,715,623 + -0.5 2,715,623 + +2.8 2,715,623 + -0.3 2,715,624 - -0.3 2,715,663 + +1.0 2,715,663 + -0.2 2,715,663 + -0.8 2,715,664 - -1.0 2,715,664 - +1.0 2,715,664 - +1.0 2,715,664 - +1.4 2,715,664 - -1.0 2,715,776 + Psyr_2338 0.18 -0.6 2,715,776 + Psyr_2338 0.18 +0.2 2,715,776 + Psyr_2338 0.18 +0.3 2,715,776 + Psyr_2338 0.18 +0.2 2,715,777 - Psyr_2338 0.18 +1.5 2,715,777 - Psyr_2338 0.18 -1.0 2,715,777 - Psyr_2338 0.18 +1.6 2,715,897 + Psyr_2338 0.35 +0.9 2,715,897 + Psyr_2338 0.35 +0.3 2,715,897 + Psyr_2338 0.35 -1.8 2,715,897 + Psyr_2338 0.35 +0.0 2,715,897 + Psyr_2338 0.35 -1.7 2,715,897 + Psyr_2338 0.35 +1.5 2,715,898 - Psyr_2338 0.35 +0.1 2,715,898 - Psyr_2338 0.35 -0.9 2,715,898 - Psyr_2338 0.35 -1.0 2,715,898 - Psyr_2338 0.35 -3.3 2,715,898 - Psyr_2338 0.35 +0.5 2,715,986 - Psyr_2338 0.48 -0.3 2,716,406 + +0.0 2,716,407 - -2.0 2,716,442 - Psyr_2339 0.16 -2.2 2,716,442 - Psyr_2339 0.16 +1.6 2,716,442 - Psyr_2339 0.16 +0.7 2,716,454 + Psyr_2339 0.21 -0.1 2,716,454 + Psyr_2339 0.21 +2.0 2,716,481 + Psyr_2339 0.33 -0.5 2,716,482 - Psyr_2339 0.34 -2.4
Or see this region's nucleotide sequence