Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2313 and Psyr_2314 are separated by 69 nucleotides Psyr_2314 and Psyr_2315 are separated by 370 nucleotides
Psyr_2313: Psyr_2313 - peptidoglycan glycosyltransferase / cell elongation-specific peptidoglycan D,D-transpeptidase, at 2,686,551 to 2,688,440
_2313
Psyr_2314: Psyr_2314 - pyruvate dehydrogenase (cytochrome), at 2,688,510 to 2,690,315
_2314
Psyr_2315: Psyr_2315 - methyl-accepting chemotaxis sensory transducer, at 2,690,686 to 2,692,647
_2315
Position (kb)
2688
2689
2690
2691 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2687.607 kb on + strand, within Psyr_2313 at 2687.608 kb on - strand, within Psyr_2313 at 2687.637 kb on + strand, within Psyr_2313 at 2687.637 kb on + strand, within Psyr_2313 at 2687.638 kb on - strand, within Psyr_2313 at 2687.642 kb on + strand, within Psyr_2313 at 2687.643 kb on - strand, within Psyr_2313 at 2687.709 kb on + strand, within Psyr_2313 at 2687.709 kb on + strand, within Psyr_2313 at 2687.709 kb on + strand, within Psyr_2313 at 2687.709 kb on + strand, within Psyr_2313 at 2687.710 kb on - strand, within Psyr_2313 at 2687.710 kb on - strand, within Psyr_2313 at 2687.710 kb on - strand, within Psyr_2313 at 2687.710 kb on - strand, within Psyr_2313 at 2687.710 kb on - strand, within Psyr_2313 at 2687.710 kb on - strand, within Psyr_2313 at 2687.710 kb on - strand, within Psyr_2313 at 2687.710 kb on - strand, within Psyr_2313 at 2687.757 kb on + strand, within Psyr_2313 at 2687.757 kb on + strand, within Psyr_2313 at 2687.757 kb on + strand, within Psyr_2313 at 2687.757 kb on + strand, within Psyr_2313 at 2687.757 kb on + strand, within Psyr_2313 at 2687.758 kb on - strand, within Psyr_2313 at 2687.758 kb on - strand, within Psyr_2313 at 2687.758 kb on - strand, within Psyr_2313 at 2687.758 kb on - strand, within Psyr_2313 at 2687.758 kb on - strand, within Psyr_2313 at 2687.907 kb on + strand, within Psyr_2313 at 2687.908 kb on - strand, within Psyr_2313 at 2688.038 kb on + strand, within Psyr_2313 at 2688.205 kb on - strand, within Psyr_2313 at 2688.205 kb on - strand, within Psyr_2313 at 2688.399 kb on + strand at 2688.399 kb on + strand at 2688.399 kb on + strand at 2688.559 kb on - strand at 2688.571 kb on - strand at 2688.571 kb on - strand at 2688.852 kb on + strand, within Psyr_2314 at 2688.853 kb on - strand, within Psyr_2314 at 2688.853 kb on - strand, within Psyr_2314 at 2688.853 kb on - strand, within Psyr_2314 at 2688.927 kb on + strand, within Psyr_2314 at 2689.317 kb on + strand, within Psyr_2314 at 2689.317 kb on + strand, within Psyr_2314 at 2689.317 kb on + strand, within Psyr_2314 at 2689.318 kb on - strand, within Psyr_2314 at 2689.438 kb on - strand, within Psyr_2314 at 2689.465 kb on + strand, within Psyr_2314 at 2689.465 kb on + strand, within Psyr_2314 at 2689.465 kb on + strand, within Psyr_2314 at 2689.465 kb on + strand, within Psyr_2314 at 2689.681 kb on - strand, within Psyr_2314 at 2690.022 kb on + strand, within Psyr_2314 at 2690.023 kb on - strand, within Psyr_2314 at 2690.023 kb on - strand, within Psyr_2314 at 2690.244 kb on + strand at 2690.244 kb on + strand at 2690.244 kb on + strand at 2690.244 kb on + strand at 2690.244 kb on + strand at 2690.316 kb on + strand at 2690.316 kb on + strand at 2690.316 kb on + strand at 2690.316 kb on + strand at 2690.317 kb on - strand at 2690.317 kb on - strand at 2690.321 kb on + strand at 2690.322 kb on - strand at 2690.322 kb on - strand at 2690.324 kb on - strand at 2690.342 kb on + strand at 2690.412 kb on + strand at 2690.497 kb on - strand at 2690.497 kb on - strand at 2690.519 kb on - strand at 2690.519 kb on - strand at 2690.656 kb on + strand at 2690.710 kb on - strand at 2690.729 kb on + strand at 2690.802 kb on + strand at 2690.802 kb on + strand at 2690.995 kb on - strand, within Psyr_2315 at 2691.016 kb on + strand, within Psyr_2315 at 2691.091 kb on - strand, within Psyr_2315 at 2691.133 kb on + strand, within Psyr_2315 at 2691.134 kb on - strand, within Psyr_2315 at 2691.167 kb on + strand, within Psyr_2315 at 2691.168 kb on - strand, within Psyr_2315
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 2,687,607 + Psyr_2313 0.56 +1.0 2,687,608 - Psyr_2313 0.56 -0.6 2,687,637 + Psyr_2313 0.57 -3.3 2,687,637 + Psyr_2313 0.57 +0.8 2,687,638 - Psyr_2313 0.58 -0.2 2,687,642 + Psyr_2313 0.58 +1.9 2,687,643 - Psyr_2313 0.58 +0.4 2,687,709 + Psyr_2313 0.61 -1.4 2,687,709 + Psyr_2313 0.61 -1.3 2,687,709 + Psyr_2313 0.61 +1.6 2,687,709 + Psyr_2313 0.61 -0.3 2,687,710 - Psyr_2313 0.61 +1.0 2,687,710 - Psyr_2313 0.61 -2.4 2,687,710 - Psyr_2313 0.61 +2.6 2,687,710 - Psyr_2313 0.61 +1.6 2,687,710 - Psyr_2313 0.61 +1.0 2,687,710 - Psyr_2313 0.61 +0.9 2,687,710 - Psyr_2313 0.61 +1.0 2,687,710 - Psyr_2313 0.61 -0.6 2,687,757 + Psyr_2313 0.64 -1.6 2,687,757 + Psyr_2313 0.64 -0.2 2,687,757 + Psyr_2313 0.64 +0.9 2,687,757 + Psyr_2313 0.64 +0.7 2,687,757 + Psyr_2313 0.64 -1.0 2,687,758 - Psyr_2313 0.64 -0.6 2,687,758 - Psyr_2313 0.64 +0.0 2,687,758 - Psyr_2313 0.64 -1.6 2,687,758 - Psyr_2313 0.64 -0.0 2,687,758 - Psyr_2313 0.64 +0.0 2,687,907 + Psyr_2313 0.72 +0.5 2,687,908 - Psyr_2313 0.72 -0.1 2,688,038 + Psyr_2313 0.79 -0.3 2,688,205 - Psyr_2313 0.88 -3.2 2,688,205 - Psyr_2313 0.88 +0.4 2,688,399 + -1.6 2,688,399 + +0.4 2,688,399 + +0.4 2,688,559 - +0.2 2,688,571 - +0.3 2,688,571 - -2.0 2,688,852 + Psyr_2314 0.19 -1.6 2,688,853 - Psyr_2314 0.19 -0.1 2,688,853 - Psyr_2314 0.19 -0.7 2,688,853 - Psyr_2314 0.19 +0.6 2,688,927 + Psyr_2314 0.23 -2.8 2,689,317 + Psyr_2314 0.45 -0.8 2,689,317 + Psyr_2314 0.45 -1.2 2,689,317 + Psyr_2314 0.45 +0.0 2,689,318 - Psyr_2314 0.45 -0.7 2,689,438 - Psyr_2314 0.51 +0.0 2,689,465 + Psyr_2314 0.53 +0.2 2,689,465 + Psyr_2314 0.53 +0.8 2,689,465 + Psyr_2314 0.53 -1.6 2,689,465 + Psyr_2314 0.53 -2.4 2,689,681 - Psyr_2314 0.65 +1.0 2,690,022 + Psyr_2314 0.84 -1.0 2,690,023 - Psyr_2314 0.84 -3.6 2,690,023 - Psyr_2314 0.84 +0.6 2,690,244 + -0.4 2,690,244 + +0.4 2,690,244 + +0.9 2,690,244 + +0.8 2,690,244 + +1.0 2,690,316 + +0.6 2,690,316 + +1.3 2,690,316 + +3.1 2,690,316 + +1.3 2,690,317 - -0.4 2,690,317 - -0.6 2,690,321 + -1.8 2,690,322 - -0.6 2,690,322 - +0.2 2,690,324 - -1.3 2,690,342 + -3.0 2,690,412 + -1.6 2,690,497 - +2.8 2,690,497 - +1.4 2,690,519 - -0.4 2,690,519 - -0.7 2,690,656 + -0.7 2,690,710 - +0.6 2,690,729 + +0.7 2,690,802 + +0.7 2,690,802 + -2.1 2,690,995 - Psyr_2315 0.16 -1.2 2,691,016 + Psyr_2315 0.17 -1.6 2,691,091 - Psyr_2315 0.21 +1.8 2,691,133 + Psyr_2315 0.23 +0.4 2,691,134 - Psyr_2315 0.23 -0.4 2,691,167 + Psyr_2315 0.25 -2.0 2,691,168 - Psyr_2315 0.25 +0.7
Or see this region's nucleotide sequence