Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2222 and Psyr_2223 overlap by 4 nucleotides Psyr_2223 and Psyr_2224 are separated by 13 nucleotides
Psyr_2222: Psyr_2222 - aminomethyltransferase, at 2,582,408 to 2,585,314
_2222
Psyr_2223: Psyr_2223 - sarcosine oxidase, delta subunit, at 2,585,311 to 2,585,616
_2223
Psyr_2224: Psyr_2224 - FAD dependent oxidoreductase, at 2,585,630 to 2,586,871
_2224
Position (kb)
2585
2586 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2584.360 kb on - strand, within Psyr_2222 at 2584.413 kb on + strand, within Psyr_2222 at 2584.414 kb on - strand, within Psyr_2222 at 2584.537 kb on - strand, within Psyr_2222 at 2584.567 kb on - strand, within Psyr_2222 at 2584.567 kb on - strand, within Psyr_2222 at 2584.567 kb on - strand, within Psyr_2222 at 2584.567 kb on - strand, within Psyr_2222 at 2584.696 kb on - strand, within Psyr_2222 at 2584.696 kb on - strand, within Psyr_2222 at 2584.824 kb on + strand, within Psyr_2222 at 2584.825 kb on - strand, within Psyr_2222 at 2584.825 kb on - strand, within Psyr_2222 at 2584.825 kb on - strand, within Psyr_2222 at 2584.825 kb on - strand, within Psyr_2222 at 2584.840 kb on - strand, within Psyr_2222 at 2584.929 kb on + strand, within Psyr_2222 at 2584.929 kb on + strand, within Psyr_2222 at 2584.930 kb on - strand, within Psyr_2222 at 2584.945 kb on - strand, within Psyr_2222 at 2585.069 kb on + strand at 2585.070 kb on - strand at 2585.148 kb on + strand at 2585.148 kb on + strand at 2585.148 kb on + strand at 2585.148 kb on + strand at 2585.226 kb on + strand at 2585.226 kb on + strand at 2585.498 kb on + strand, within Psyr_2223 at 2585.499 kb on - strand, within Psyr_2223 at 2585.808 kb on + strand, within Psyr_2224 at 2585.808 kb on + strand, within Psyr_2224 at 2585.811 kb on + strand, within Psyr_2224 at 2585.812 kb on - strand at 2585.812 kb on - strand, within Psyr_2224 at 2585.847 kb on + strand, within Psyr_2224 at 2586.036 kb on + strand, within Psyr_2224 at 2586.036 kb on + strand, within Psyr_2224 at 2586.036 kb on + strand, within Psyr_2224 at 2586.037 kb on - strand, within Psyr_2224 at 2586.037 kb on - strand, within Psyr_2224 at 2586.037 kb on - strand, within Psyr_2224 at 2586.037 kb on - strand, within Psyr_2224 at 2586.252 kb on - strand, within Psyr_2224 at 2586.252 kb on - strand, within Psyr_2224 at 2586.252 kb on - strand, within Psyr_2224 at 2586.252 kb on - strand, within Psyr_2224 at 2586.252 kb on - strand, within Psyr_2224 at 2586.543 kb on + strand, within Psyr_2224 at 2586.543 kb on + strand, within Psyr_2224 at 2586.543 kb on + strand, within Psyr_2224 at 2586.543 kb on + strand, within Psyr_2224 at 2586.543 kb on + strand, within Psyr_2224 at 2586.543 kb on + strand, within Psyr_2224 at 2586.543 kb on + strand, within Psyr_2224 at 2586.543 kb on + strand, within Psyr_2224 at 2586.544 kb on - strand, within Psyr_2224 at 2586.603 kb on + strand, within Psyr_2224 at 2586.603 kb on + strand, within Psyr_2224 at 2586.603 kb on + strand, within Psyr_2224 at 2586.603 kb on + strand, within Psyr_2224 at 2586.603 kb on + strand, within Psyr_2224 at 2586.604 kb on - strand, within Psyr_2224 at 2586.604 kb on - strand, within Psyr_2224
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 2,584,360 - Psyr_2222 0.67 -0.6 2,584,413 + Psyr_2222 0.69 -1.6 2,584,414 - Psyr_2222 0.69 +0.0 2,584,537 - Psyr_2222 0.73 +1.0 2,584,567 - Psyr_2222 0.74 +1.5 2,584,567 - Psyr_2222 0.74 +0.1 2,584,567 - Psyr_2222 0.74 -2.1 2,584,567 - Psyr_2222 0.74 -2.9 2,584,696 - Psyr_2222 0.79 -0.6 2,584,696 - Psyr_2222 0.79 -2.1 2,584,824 + Psyr_2222 0.83 -0.9 2,584,825 - Psyr_2222 0.83 +0.3 2,584,825 - Psyr_2222 0.83 -0.2 2,584,825 - Psyr_2222 0.83 +1.4 2,584,825 - Psyr_2222 0.83 +0.9 2,584,840 - Psyr_2222 0.84 -1.1 2,584,929 + Psyr_2222 0.87 +0.9 2,584,929 + Psyr_2222 0.87 +2.6 2,584,930 - Psyr_2222 0.87 +1.3 2,584,945 - Psyr_2222 0.87 +1.0 2,585,069 + +0.8 2,585,070 - -0.2 2,585,148 + +1.0 2,585,148 + -1.2 2,585,148 + -1.6 2,585,148 + +0.0 2,585,226 + +0.5 2,585,226 + -0.6 2,585,498 + Psyr_2223 0.61 -0.1 2,585,499 - Psyr_2223 0.61 -1.8 2,585,808 + Psyr_2224 0.14 -0.4 2,585,808 + Psyr_2224 0.14 +1.6 2,585,811 + Psyr_2224 0.15 +0.8 2,585,812 - +3.0 2,585,812 - Psyr_2224 0.15 -0.3 2,585,847 + Psyr_2224 0.17 -1.0 2,586,036 + Psyr_2224 0.33 -0.7 2,586,036 + Psyr_2224 0.33 +0.6 2,586,036 + Psyr_2224 0.33 +0.3 2,586,037 - Psyr_2224 0.33 -0.2 2,586,037 - Psyr_2224 0.33 +1.4 2,586,037 - Psyr_2224 0.33 -0.9 2,586,037 - Psyr_2224 0.33 -1.4 2,586,252 - Psyr_2224 0.50 +0.1 2,586,252 - Psyr_2224 0.50 +0.3 2,586,252 - Psyr_2224 0.50 +0.6 2,586,252 - Psyr_2224 0.50 -0.1 2,586,252 - Psyr_2224 0.50 +1.4 2,586,543 + Psyr_2224 0.74 -2.8 2,586,543 + Psyr_2224 0.74 -0.0 2,586,543 + Psyr_2224 0.74 +0.8 2,586,543 + Psyr_2224 0.74 -0.0 2,586,543 + Psyr_2224 0.74 +0.5 2,586,543 + Psyr_2224 0.74 -1.4 2,586,543 + Psyr_2224 0.74 +3.3 2,586,543 + Psyr_2224 0.74 -1.7 2,586,544 - Psyr_2224 0.74 -0.3 2,586,603 + Psyr_2224 0.78 +2.2 2,586,603 + Psyr_2224 0.78 +1.0 2,586,603 + Psyr_2224 0.78 +1.3 2,586,603 + Psyr_2224 0.78 -0.0 2,586,603 + Psyr_2224 0.78 -0.7 2,586,604 - Psyr_2224 0.78 -1.2 2,586,604 - Psyr_2224 0.78 -0.1
Or see this region's nucleotide sequence