Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2085 and Psyr_2086 are separated by 159 nucleotides Psyr_2086 and Psyr_2087 are separated by 68 nucleotides
Psyr_2085: Psyr_2085 - methylisocitrate lyase, at 2,430,393 to 2,431,286
_2085
Psyr_2086: Psyr_2086 - 2-methylcitrate synthase, at 2,431,446 to 2,432,573
_2086
Psyr_2087: Psyr_2087 - aconitase / 2-methylcitrate dehydratase (trans-methylaconitate-forming), at 2,432,642 to 2,435,230
_2087
Position (kb)
2431
2432
2433 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2430.521 kb on + strand, within Psyr_2085 at 2430.522 kb on - strand, within Psyr_2085 at 2430.525 kb on + strand, within Psyr_2085 at 2430.525 kb on + strand, within Psyr_2085 at 2430.525 kb on + strand, within Psyr_2085 at 2430.525 kb on + strand, within Psyr_2085 at 2430.525 kb on + strand, within Psyr_2085 at 2430.526 kb on - strand, within Psyr_2085 at 2430.526 kb on - strand, within Psyr_2085 at 2430.598 kb on + strand, within Psyr_2085 at 2430.599 kb on - strand, within Psyr_2085 at 2430.599 kb on - strand, within Psyr_2085 at 2430.930 kb on - strand, within Psyr_2085 at 2430.948 kb on - strand, within Psyr_2085 at 2430.984 kb on + strand, within Psyr_2085 at 2430.984 kb on + strand, within Psyr_2085 at 2430.984 kb on + strand, within Psyr_2085 at 2430.985 kb on - strand, within Psyr_2085 at 2431.059 kb on - strand, within Psyr_2085 at 2431.218 kb on + strand at 2431.219 kb on - strand at 2431.219 kb on - strand at 2431.352 kb on + strand at 2431.352 kb on + strand at 2431.353 kb on - strand at 2431.382 kb on + strand at 2431.462 kb on + strand at 2431.545 kb on + strand at 2431.602 kb on + strand, within Psyr_2086 at 2431.650 kb on + strand, within Psyr_2086 at 2431.650 kb on + strand, within Psyr_2086 at 2431.650 kb on + strand, within Psyr_2086 at 2431.650 kb on + strand, within Psyr_2086 at 2431.650 kb on + strand, within Psyr_2086 at 2431.650 kb on + strand, within Psyr_2086 at 2431.651 kb on - strand, within Psyr_2086 at 2431.651 kb on - strand, within Psyr_2086 at 2431.651 kb on - strand, within Psyr_2086 at 2431.651 kb on - strand, within Psyr_2086 at 2431.651 kb on - strand, within Psyr_2086 at 2431.651 kb on - strand, within Psyr_2086 at 2431.653 kb on - strand, within Psyr_2086 at 2432.065 kb on - strand, within Psyr_2086 at 2432.236 kb on - strand, within Psyr_2086 at 2432.385 kb on + strand, within Psyr_2086 at 2432.385 kb on + strand, within Psyr_2086 at 2432.386 kb on - strand, within Psyr_2086 at 2432.526 kb on + strand at 2432.526 kb on + strand at 2432.526 kb on + strand at 2432.527 kb on - strand at 2432.571 kb on + strand at 2432.688 kb on - strand at 2432.732 kb on + strand at 2432.747 kb on + strand at 2432.747 kb on + strand at 2432.747 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 2,430,521 + Psyr_2085 0.14 +1.1 2,430,522 - Psyr_2085 0.14 +0.8 2,430,525 + Psyr_2085 0.15 +0.6 2,430,525 + Psyr_2085 0.15 +2.5 2,430,525 + Psyr_2085 0.15 +0.0 2,430,525 + Psyr_2085 0.15 -0.3 2,430,525 + Psyr_2085 0.15 -0.6 2,430,526 - Psyr_2085 0.15 +0.5 2,430,526 - Psyr_2085 0.15 -1.7 2,430,598 + Psyr_2085 0.23 +1.4 2,430,599 - Psyr_2085 0.23 +1.6 2,430,599 - Psyr_2085 0.23 +0.1 2,430,930 - Psyr_2085 0.60 -3.5 2,430,948 - Psyr_2085 0.62 -0.5 2,430,984 + Psyr_2085 0.66 -0.6 2,430,984 + Psyr_2085 0.66 +0.6 2,430,984 + Psyr_2085 0.66 +2.9 2,430,985 - Psyr_2085 0.66 -0.4 2,431,059 - Psyr_2085 0.74 -1.0 2,431,218 + +1.9 2,431,219 - -1.1 2,431,219 - -1.7 2,431,352 + +1.3 2,431,352 + +0.2 2,431,353 - +1.3 2,431,382 + -0.1 2,431,462 + +0.3 2,431,545 + -1.4 2,431,602 + Psyr_2086 0.14 +0.3 2,431,650 + Psyr_2086 0.18 +0.2 2,431,650 + Psyr_2086 0.18 +0.5 2,431,650 + Psyr_2086 0.18 +2.5 2,431,650 + Psyr_2086 0.18 -3.2 2,431,650 + Psyr_2086 0.18 -0.8 2,431,650 + Psyr_2086 0.18 -1.3 2,431,651 - Psyr_2086 0.18 -0.4 2,431,651 - Psyr_2086 0.18 +0.4 2,431,651 - Psyr_2086 0.18 -0.6 2,431,651 - Psyr_2086 0.18 -1.1 2,431,651 - Psyr_2086 0.18 -0.3 2,431,651 - Psyr_2086 0.18 -2.8 2,431,653 - Psyr_2086 0.18 -1.7 2,432,065 - Psyr_2086 0.55 -2.2 2,432,236 - Psyr_2086 0.70 +0.6 2,432,385 + Psyr_2086 0.83 -0.9 2,432,385 + Psyr_2086 0.83 -1.3 2,432,386 - Psyr_2086 0.83 -1.3 2,432,526 + -1.0 2,432,526 + -0.1 2,432,526 + -2.1 2,432,527 - -2.9 2,432,571 + -0.4 2,432,688 - +0.3 2,432,732 + -0.5 2,432,747 + -0.1 2,432,747 + -1.1 2,432,747 + -1.5
Or see this region's nucleotide sequence