Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2028 and Psyr_2029 are separated by 69 nucleotides Psyr_2029 and Psyr_2030 are separated by 45 nucleotides Psyr_2030 and Psyr_2031 are separated by 115 nucleotides
Psyr_2028: Psyr_2028 - conserved hypothetical protein, at 2,352,422 to 2,353,252
_2028
Psyr_2029: Psyr_2029 - Protein of unknown function UPF0118, at 2,353,322 to 2,354,368
_2029
Psyr_2030: Psyr_2030 - Calcium-binding EF-hand, at 2,354,414 to 2,355,169
_2030
Psyr_2031: Psyr_2031 - ATP-binding region, ATPase-like:Histidine kinase, HAMP region:Histidine kinase A, N-terminal, at 2,355,285 to 2,356,319
_2031
Position (kb)
2353
2354
2355 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2352.394 kb on + strand at 2352.394 kb on + strand at 2352.395 kb on - strand at 2352.507 kb on + strand, within Psyr_2028 at 2352.507 kb on + strand, within Psyr_2028 at 2352.508 kb on - strand, within Psyr_2028 at 2352.508 kb on - strand, within Psyr_2028 at 2352.508 kb on - strand, within Psyr_2028 at 2352.684 kb on - strand, within Psyr_2028 at 2352.690 kb on + strand, within Psyr_2028 at 2352.690 kb on + strand, within Psyr_2028 at 2352.691 kb on - strand, within Psyr_2028 at 2352.691 kb on - strand, within Psyr_2028 at 2352.691 kb on - strand, within Psyr_2028 at 2352.839 kb on + strand, within Psyr_2028 at 2352.840 kb on - strand, within Psyr_2028 at 2352.914 kb on + strand, within Psyr_2028 at 2352.914 kb on + strand, within Psyr_2028 at 2352.915 kb on - strand, within Psyr_2028 at 2352.944 kb on + strand, within Psyr_2028 at 2352.945 kb on - strand, within Psyr_2028 at 2353.084 kb on + strand, within Psyr_2028 at 2353.250 kb on + strand at 2353.284 kb on + strand at 2353.285 kb on - strand at 2353.835 kb on + strand, within Psyr_2029 at 2353.836 kb on - strand, within Psyr_2029 at 2353.907 kb on + strand, within Psyr_2029 at 2353.907 kb on + strand, within Psyr_2029 at 2353.908 kb on - strand, within Psyr_2029 at 2353.945 kb on - strand, within Psyr_2029 at 2354.198 kb on + strand, within Psyr_2029 at 2354.198 kb on + strand, within Psyr_2029 at 2354.199 kb on - strand, within Psyr_2029 at 2354.199 kb on - strand, within Psyr_2029 at 2354.199 kb on - strand, within Psyr_2029 at 2354.223 kb on + strand, within Psyr_2029 at 2354.319 kb on - strand at 2354.467 kb on - strand at 2354.467 kb on - strand at 2354.587 kb on + strand, within Psyr_2030 at 2354.615 kb on - strand, within Psyr_2030 at 2354.615 kb on - strand, within Psyr_2030 at 2354.672 kb on - strand, within Psyr_2030 at 2355.135 kb on + strand at 2355.136 kb on - strand at 2355.155 kb on + strand at 2355.253 kb on + strand at 2355.253 kb on + strand at 2355.254 kb on - strand at 2355.254 kb on - strand at 2355.254 kb on - strand at 2355.254 kb on - strand at 2355.254 kb on - strand at 2355.262 kb on + strand at 2355.262 kb on + strand at 2355.262 kb on + strand at 2355.262 kb on + strand at 2355.263 kb on - strand at 2355.263 kb on - strand at 2355.281 kb on + strand at 2355.281 kb on + strand at 2355.281 kb on + strand at 2355.281 kb on + strand at 2355.281 kb on + strand at 2355.282 kb on - strand at 2355.282 kb on - strand at 2355.282 kb on - strand at 2355.282 kb on - strand at 2355.282 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 2,352,394 + +1.2 2,352,394 + -0.8 2,352,395 - +0.4 2,352,507 + Psyr_2028 0.10 -0.5 2,352,507 + Psyr_2028 0.10 -0.4 2,352,508 - Psyr_2028 0.10 -0.4 2,352,508 - Psyr_2028 0.10 +0.9 2,352,508 - Psyr_2028 0.10 -2.6 2,352,684 - Psyr_2028 0.32 -0.3 2,352,690 + Psyr_2028 0.32 -0.3 2,352,690 + Psyr_2028 0.32 -2.4 2,352,691 - Psyr_2028 0.32 +0.4 2,352,691 - Psyr_2028 0.32 -0.2 2,352,691 - Psyr_2028 0.32 -0.7 2,352,839 + Psyr_2028 0.50 -0.6 2,352,840 - Psyr_2028 0.50 -1.0 2,352,914 + Psyr_2028 0.59 -1.7 2,352,914 + Psyr_2028 0.59 -0.9 2,352,915 - Psyr_2028 0.59 -2.6 2,352,944 + Psyr_2028 0.63 -0.7 2,352,945 - Psyr_2028 0.63 +0.2 2,353,084 + Psyr_2028 0.80 +0.0 2,353,250 + -0.2 2,353,284 + +0.1 2,353,285 - -0.7 2,353,835 + Psyr_2029 0.49 +0.9 2,353,836 - Psyr_2029 0.49 +0.2 2,353,907 + Psyr_2029 0.56 +2.2 2,353,907 + Psyr_2029 0.56 +0.8 2,353,908 - Psyr_2029 0.56 +0.9 2,353,945 - Psyr_2029 0.60 +1.3 2,354,198 + Psyr_2029 0.84 +1.2 2,354,198 + Psyr_2029 0.84 -0.2 2,354,199 - Psyr_2029 0.84 -2.9 2,354,199 - Psyr_2029 0.84 +1.1 2,354,199 - Psyr_2029 0.84 -1.1 2,354,223 + Psyr_2029 0.86 +0.3 2,354,319 - -1.1 2,354,467 - +1.0 2,354,467 - -1.1 2,354,587 + Psyr_2030 0.23 -0.3 2,354,615 - Psyr_2030 0.27 +0.0 2,354,615 - Psyr_2030 0.27 -1.2 2,354,672 - Psyr_2030 0.34 -2.4 2,355,135 + +1.1 2,355,136 - +1.7 2,355,155 + -1.0 2,355,253 + +0.7 2,355,253 + -0.4 2,355,254 - -0.2 2,355,254 - -0.2 2,355,254 - -1.1 2,355,254 - +0.8 2,355,254 - +2.5 2,355,262 + -1.9 2,355,262 + +1.6 2,355,262 + -1.9 2,355,262 + +1.2 2,355,263 - +0.7 2,355,263 - -0.0 2,355,281 + +1.7 2,355,281 + +0.7 2,355,281 + -0.9 2,355,281 + -0.1 2,355,281 + +1.9 2,355,282 - +0.9 2,355,282 - -0.1 2,355,282 - -0.4 2,355,282 - +0.1 2,355,282 - +0.5
Or see this region's nucleotide sequence