Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2017 and Psyr_2018 are separated by 90 nucleotides Psyr_2018 and Psyr_2019 are separated by 520 nucleotides
Psyr_2017: Psyr_2017 - Heat shock protein Hsp90:ATP-binding region, ATPase-like protein, at 2,340,585 to 2,342,498
_2017
Psyr_2018: Psyr_2018 - Dienelactone hydrolase, at 2,342,589 to 2,343,317
_2018
Psyr_2019: Psyr_2019 - 3-oxoacyl-[acyl-carrier-protein] synthase I, at 2,343,838 to 2,345,061
_2019
Position (kb)
2342
2343
2344 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2341.722 kb on + strand, within Psyr_2017 at 2341.723 kb on - strand, within Psyr_2017 at 2341.723 kb on - strand, within Psyr_2017 at 2341.723 kb on - strand, within Psyr_2017 at 2341.903 kb on - strand, within Psyr_2017 at 2341.905 kb on + strand, within Psyr_2017 at 2341.906 kb on - strand, within Psyr_2017 at 2341.924 kb on - strand, within Psyr_2017 at 2342.023 kb on - strand, within Psyr_2017 at 2342.312 kb on + strand at 2342.313 kb on - strand at 2342.313 kb on - strand at 2342.454 kb on + strand at 2342.483 kb on - strand at 2342.483 kb on - strand at 2342.643 kb on + strand at 2342.644 kb on - strand at 2342.644 kb on - strand at 2342.644 kb on - strand at 2342.660 kb on - strand at 2342.661 kb on + strand at 2342.662 kb on - strand, within Psyr_2018 at 2342.662 kb on - strand, within Psyr_2018 at 2342.662 kb on - strand, within Psyr_2018 at 2342.794 kb on - strand, within Psyr_2018 at 2342.816 kb on + strand, within Psyr_2018 at 2342.935 kb on + strand, within Psyr_2018 at 2342.935 kb on + strand, within Psyr_2018 at 2342.935 kb on + strand, within Psyr_2018 at 2342.936 kb on - strand, within Psyr_2018 at 2342.936 kb on - strand, within Psyr_2018 at 2343.181 kb on + strand, within Psyr_2018 at 2343.181 kb on + strand, within Psyr_2018 at 2343.181 kb on + strand, within Psyr_2018 at 2343.181 kb on + strand, within Psyr_2018 at 2343.181 kb on + strand, within Psyr_2018 at 2343.182 kb on - strand, within Psyr_2018 at 2343.182 kb on - strand, within Psyr_2018 at 2343.182 kb on - strand, within Psyr_2018 at 2343.182 kb on - strand, within Psyr_2018 at 2343.182 kb on - strand, within Psyr_2018 at 2343.183 kb on + strand, within Psyr_2018 at 2343.183 kb on + strand, within Psyr_2018 at 2343.184 kb on - strand, within Psyr_2018 at 2343.184 kb on - strand, within Psyr_2018 at 2343.184 kb on - strand, within Psyr_2018 at 2343.189 kb on + strand, within Psyr_2018 at 2343.190 kb on - strand, within Psyr_2018 at 2343.249 kb on + strand at 2343.249 kb on + strand at 2343.249 kb on + strand at 2343.249 kb on + strand at 2343.249 kb on + strand at 2343.249 kb on + strand at 2343.249 kb on + strand at 2343.249 kb on + strand at 2343.249 kb on + strand at 2343.250 kb on - strand at 2343.250 kb on - strand at 2343.250 kb on - strand at 2343.250 kb on - strand at 2343.750 kb on - strand at 2343.750 kb on - strand at 2343.750 kb on - strand at 2343.753 kb on + strand at 2343.753 kb on + strand at 2343.753 kb on + strand at 2343.753 kb on + strand at 2343.754 kb on - strand at 2343.754 kb on - strand at 2343.754 kb on - strand at 2343.768 kb on + strand at 2343.769 kb on - strand at 2343.769 kb on - strand at 2343.836 kb on + strand at 2343.839 kb on + strand at 2343.840 kb on - strand at 2343.840 kb on - strand at 2343.840 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 2,341,722 + Psyr_2017 0.59 +1.3 2,341,723 - Psyr_2017 0.59 -2.4 2,341,723 - Psyr_2017 0.59 +0.5 2,341,723 - Psyr_2017 0.59 +1.2 2,341,903 - Psyr_2017 0.69 -2.4 2,341,905 + Psyr_2017 0.69 +0.8 2,341,906 - Psyr_2017 0.69 +0.5 2,341,924 - Psyr_2017 0.70 -2.1 2,342,023 - Psyr_2017 0.75 -2.8 2,342,312 + +0.3 2,342,313 - -1.7 2,342,313 - -1.1 2,342,454 + +0.4 2,342,483 - +0.7 2,342,483 - -3.4 2,342,643 + +1.7 2,342,644 - -2.7 2,342,644 - -1.0 2,342,644 - -2.6 2,342,660 - -0.4 2,342,661 + -1.9 2,342,662 - Psyr_2018 0.10 -1.4 2,342,662 - Psyr_2018 0.10 -1.5 2,342,662 - Psyr_2018 0.10 +0.2 2,342,794 - Psyr_2018 0.28 -2.0 2,342,816 + Psyr_2018 0.31 -2.6 2,342,935 + Psyr_2018 0.47 -2.1 2,342,935 + Psyr_2018 0.47 +0.7 2,342,935 + Psyr_2018 0.47 -1.1 2,342,936 - Psyr_2018 0.48 +1.5 2,342,936 - Psyr_2018 0.48 -2.3 2,343,181 + Psyr_2018 0.81 -0.5 2,343,181 + Psyr_2018 0.81 -1.4 2,343,181 + Psyr_2018 0.81 -0.1 2,343,181 + Psyr_2018 0.81 -1.5 2,343,181 + Psyr_2018 0.81 +0.9 2,343,182 - Psyr_2018 0.81 +1.9 2,343,182 - Psyr_2018 0.81 -1.0 2,343,182 - Psyr_2018 0.81 +0.9 2,343,182 - Psyr_2018 0.81 -1.2 2,343,182 - Psyr_2018 0.81 +0.3 2,343,183 + Psyr_2018 0.81 -0.1 2,343,183 + Psyr_2018 0.81 +0.9 2,343,184 - Psyr_2018 0.82 -2.1 2,343,184 - Psyr_2018 0.82 -2.1 2,343,184 - Psyr_2018 0.82 -1.1 2,343,189 + Psyr_2018 0.82 -1.1 2,343,190 - Psyr_2018 0.82 -1.2 2,343,249 + +1.5 2,343,249 + -1.4 2,343,249 + -3.2 2,343,249 + +0.3 2,343,249 + -0.7 2,343,249 + +0.3 2,343,249 + -1.6 2,343,249 + -1.6 2,343,249 + +0.4 2,343,250 - +0.3 2,343,250 - -1.7 2,343,250 - -0.8 2,343,250 - -1.8 2,343,750 - -0.7 2,343,750 - -0.8 2,343,750 - -1.9 2,343,753 + -1.5 2,343,753 + +0.0 2,343,753 + +0.1 2,343,753 + +0.3 2,343,754 - -0.4 2,343,754 - -3.7 2,343,754 - -1.8 2,343,768 + -0.3 2,343,769 - -0.6 2,343,769 - -0.9 2,343,836 + -1.4 2,343,839 + -0.1 2,343,840 - +1.2 2,343,840 - -0.7 2,343,840 - +0.9
Or see this region's nucleotide sequence