Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1987 and Psyr_1988 are separated by 1 nucleotides Psyr_1988 and Psyr_1989 are separated by 12 nucleotides
Psyr_1987: Psyr_1987 - 2-keto-3-deoxygalactonate kinase, at 2,307,402 to 2,308,406
_1987
Psyr_1988: Psyr_1988 - 2-keto-3-deoxy-phosphogalactonate aldolase, at 2,308,408 to 2,309,043
_1988
Psyr_1989: Psyr_1989 - galactonate dehydratase, at 2,309,056 to 2,310,261
_1989
Position (kb)
2308
2309
2310 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2307.910 kb on + strand, within Psyr_1987 at 2307.910 kb on + strand, within Psyr_1987 at 2308.152 kb on + strand, within Psyr_1987 at 2308.269 kb on + strand, within Psyr_1987 at 2308.269 kb on + strand, within Psyr_1987 at 2308.882 kb on - strand, within Psyr_1988 at 2308.905 kb on - strand, within Psyr_1988 at 2308.905 kb on - strand, within Psyr_1988 at 2308.905 kb on - strand, within Psyr_1988 at 2308.905 kb on - strand, within Psyr_1988 at 2308.999 kb on + strand at 2308.999 kb on + strand at 2309.000 kb on - strand at 2309.000 kb on - strand at 2309.000 kb on - strand at 2309.000 kb on - strand at 2309.000 kb on - strand at 2309.000 kb on - strand at 2309.000 kb on - strand at 2309.085 kb on - strand at 2309.263 kb on + strand, within Psyr_1989 at 2309.263 kb on + strand, within Psyr_1989 at 2309.719 kb on + strand, within Psyr_1989 at 2309.720 kb on - strand, within Psyr_1989 at 2309.761 kb on + strand, within Psyr_1989 at 2309.761 kb on + strand, within Psyr_1989 at 2309.762 kb on - strand, within Psyr_1989 at 2309.762 kb on - strand, within Psyr_1989 at 2309.762 kb on - strand, within Psyr_1989 at 2309.762 kb on - strand, within Psyr_1989 at 2309.762 kb on - strand, within Psyr_1989 at 2309.762 kb on - strand, within Psyr_1989 at 2309.762 kb on - strand, within Psyr_1989 at 2309.762 kb on - strand, within Psyr_1989 at 2309.864 kb on - strand, within Psyr_1989 at 2309.944 kb on + strand, within Psyr_1989 at 2310.022 kb on + strand, within Psyr_1989 at 2310.023 kb on - strand, within Psyr_1989
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 2,307,910 + Psyr_1987 0.51 +1.6 2,307,910 + Psyr_1987 0.51 +0.5 2,308,152 + Psyr_1987 0.75 -1.1 2,308,269 + Psyr_1987 0.86 +2.1 2,308,269 + Psyr_1987 0.86 -1.8 2,308,882 - Psyr_1988 0.75 -0.9 2,308,905 - Psyr_1988 0.78 -1.8 2,308,905 - Psyr_1988 0.78 +0.1 2,308,905 - Psyr_1988 0.78 -1.1 2,308,905 - Psyr_1988 0.78 -0.8 2,308,999 + -0.7 2,308,999 + +1.5 2,309,000 - -1.6 2,309,000 - +0.0 2,309,000 - -0.1 2,309,000 - -1.6 2,309,000 - +0.3 2,309,000 - -1.5 2,309,000 - +0.9 2,309,085 - -0.9 2,309,263 + Psyr_1989 0.17 -0.1 2,309,263 + Psyr_1989 0.17 +0.5 2,309,719 + Psyr_1989 0.55 -0.3 2,309,720 - Psyr_1989 0.55 -1.1 2,309,761 + Psyr_1989 0.58 -0.4 2,309,761 + Psyr_1989 0.58 +0.9 2,309,762 - Psyr_1989 0.59 -1.7 2,309,762 - Psyr_1989 0.59 -1.1 2,309,762 - Psyr_1989 0.59 +0.2 2,309,762 - Psyr_1989 0.59 -0.1 2,309,762 - Psyr_1989 0.59 -1.1 2,309,762 - Psyr_1989 0.59 -2.8 2,309,762 - Psyr_1989 0.59 -0.7 2,309,762 - Psyr_1989 0.59 -0.5 2,309,864 - Psyr_1989 0.67 -1.1 2,309,944 + Psyr_1989 0.74 +0.3 2,310,022 + Psyr_1989 0.80 +1.4 2,310,023 - Psyr_1989 0.80 -0.6
Or see this region's nucleotide sequence