Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1830 and Psyr_1831 are separated by 59 nucleotides Psyr_1831 and Psyr_1832 are separated by 112 nucleotides Psyr_1832 and Psyr_1833 are separated by 249 nucleotides
Psyr_1830: Psyr_1830 - ABC transporter, transmembrane region:ABC transporter, at 2,110,600 to 2,112,369
_1830
Psyr_1831: Psyr_1831 - Protein of unknown function DUF1255, at 2,112,429 to 2,112,710
_1831
Psyr_1832: Psyr_1832 - Exonuclease, at 2,112,823 to 2,113,368
_1832
Psyr_1833: Psyr_1833 - amino acid ABC transporter substrate-binding protein, PAAT family, at 2,113,618 to 2,114,457
_1833
Position (kb)
2112
2113
2114 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2112.024 kb on + strand, within Psyr_1830 at 2112.202 kb on - strand at 2112.325 kb on + strand at 2112.368 kb on - strand at 2112.368 kb on - strand at 2112.368 kb on - strand at 2112.368 kb on - strand at 2112.437 kb on - strand at 2112.691 kb on + strand at 2112.692 kb on - strand at 2112.812 kb on + strand at 2112.812 kb on + strand at 2112.812 kb on + strand at 2112.812 kb on + strand at 2112.813 kb on - strand at 2112.817 kb on + strand at 2112.818 kb on - strand at 2112.858 kb on + strand at 2113.059 kb on - strand, within Psyr_1832 at 2113.059 kb on - strand, within Psyr_1832 at 2113.282 kb on + strand, within Psyr_1832 at 2113.282 kb on + strand, within Psyr_1832 at 2113.379 kb on + strand at 2113.379 kb on + strand at 2113.380 kb on - strand at 2113.457 kb on + strand at 2113.457 kb on + strand at 2113.458 kb on - strand at 2113.469 kb on + strand at 2113.470 kb on - strand at 2113.530 kb on + strand at 2113.580 kb on - strand at 2113.580 kb on - strand at 2113.597 kb on + strand at 2113.598 kb on - strand at 2113.598 kb on - strand at 2113.649 kb on + strand at 2113.649 kb on + strand at 2113.649 kb on + strand at 2113.768 kb on + strand, within Psyr_1833 at 2113.768 kb on + strand, within Psyr_1833 at 2113.768 kb on + strand, within Psyr_1833 at 2113.768 kb on + strand, within Psyr_1833 at 2113.768 kb on + strand, within Psyr_1833 at 2113.768 kb on + strand, within Psyr_1833 at 2113.769 kb on - strand, within Psyr_1833 at 2113.769 kb on - strand, within Psyr_1833 at 2113.769 kb on - strand, within Psyr_1833 at 2113.769 kb on - strand, within Psyr_1833 at 2113.769 kb on - strand, within Psyr_1833 at 2113.769 kb on - strand, within Psyr_1833 at 2113.769 kb on - strand, within Psyr_1833 at 2113.769 kb on - strand, within Psyr_1833 at 2113.769 kb on - strand, within Psyr_1833 at 2113.769 kb on - strand, within Psyr_1833 at 2113.770 kb on + strand, within Psyr_1833 at 2113.770 kb on + strand, within Psyr_1833 at 2113.770 kb on + strand, within Psyr_1833 at 2113.770 kb on + strand, within Psyr_1833 at 2113.770 kb on + strand, within Psyr_1833 at 2113.770 kb on + strand, within Psyr_1833 at 2113.770 kb on + strand, within Psyr_1833 at 2113.770 kb on + strand, within Psyr_1833 at 2113.770 kb on + strand, within Psyr_1833 at 2113.771 kb on - strand, within Psyr_1833 at 2113.771 kb on - strand, within Psyr_1833 at 2113.771 kb on - strand, within Psyr_1833 at 2113.771 kb on - strand, within Psyr_1833 at 2113.771 kb on - strand, within Psyr_1833 at 2113.771 kb on - strand, within Psyr_1833 at 2113.771 kb on - strand, within Psyr_1833 at 2113.771 kb on - strand, within Psyr_1833 at 2113.771 kb on - strand, within Psyr_1833 at 2113.771 kb on - strand, within Psyr_1833 at 2113.849 kb on + strand, within Psyr_1833 at 2113.849 kb on + strand, within Psyr_1833 at 2113.850 kb on - strand, within Psyr_1833 at 2113.850 kb on - strand, within Psyr_1833 at 2114.002 kb on + strand at 2114.002 kb on + strand, within Psyr_1833 at 2114.002 kb on + strand, within Psyr_1833 at 2114.002 kb on + strand, within Psyr_1833 at 2114.003 kb on - strand, within Psyr_1833 at 2114.003 kb on - strand, within Psyr_1833 at 2114.003 kb on - strand, within Psyr_1833 at 2114.003 kb on - strand, within Psyr_1833 at 2114.003 kb on - strand, within Psyr_1833 at 2114.003 kb on - strand, within Psyr_1833 at 2114.003 kb on - strand, within Psyr_1833 at 2114.003 kb on - strand, within Psyr_1833 at 2114.038 kb on + strand, within Psyr_1833 at 2114.038 kb on + strand, within Psyr_1833 at 2114.122 kb on + strand, within Psyr_1833 at 2114.123 kb on - strand, within Psyr_1833 at 2114.123 kb on - strand, within Psyr_1833 at 2114.145 kb on + strand, within Psyr_1833 at 2114.156 kb on - strand, within Psyr_1833 at 2114.156 kb on - strand, within Psyr_1833 at 2114.156 kb on - strand, within Psyr_1833 at 2114.225 kb on - strand, within Psyr_1833 at 2114.247 kb on + strand, within Psyr_1833 at 2114.334 kb on + strand, within Psyr_1833 at 2114.334 kb on + strand, within Psyr_1833 at 2114.335 kb on - strand, within Psyr_1833 at 2114.360 kb on + strand, within Psyr_1833
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 2,112,024 + Psyr_1830 0.80 +2.6 2,112,202 - +0.2 2,112,325 + +0.7 2,112,368 - -1.6 2,112,368 - +0.2 2,112,368 - +0.7 2,112,368 - +1.3 2,112,437 - +1.3 2,112,691 + -1.4 2,112,692 - +1.4 2,112,812 + -1.1 2,112,812 + -1.1 2,112,812 + -2.5 2,112,812 + -1.1 2,112,813 - -0.8 2,112,817 + +0.3 2,112,818 - +1.8 2,112,858 + -0.6 2,113,059 - Psyr_1832 0.43 -1.6 2,113,059 - Psyr_1832 0.43 -1.1 2,113,282 + Psyr_1832 0.84 -0.6 2,113,282 + Psyr_1832 0.84 -0.7 2,113,379 + -2.8 2,113,379 + -2.1 2,113,380 - +1.3 2,113,457 + -0.3 2,113,457 + -2.3 2,113,458 - +2.7 2,113,469 + -2.4 2,113,470 - +0.9 2,113,530 + +0.2 2,113,580 - +0.6 2,113,580 - +0.2 2,113,597 + +3.0 2,113,598 - +1.2 2,113,598 - +0.7 2,113,649 + -0.3 2,113,649 + +0.6 2,113,649 + +0.3 2,113,768 + Psyr_1833 0.18 +0.7 2,113,768 + Psyr_1833 0.18 -0.3 2,113,768 + Psyr_1833 0.18 +2.3 2,113,768 + Psyr_1833 0.18 +2.3 2,113,768 + Psyr_1833 0.18 -0.0 2,113,768 + Psyr_1833 0.18 -1.9 2,113,769 - Psyr_1833 0.18 -0.2 2,113,769 - Psyr_1833 0.18 -0.9 2,113,769 - Psyr_1833 0.18 +0.1 2,113,769 - Psyr_1833 0.18 +0.3 2,113,769 - Psyr_1833 0.18 +0.9 2,113,769 - Psyr_1833 0.18 -0.9 2,113,769 - Psyr_1833 0.18 +0.1 2,113,769 - Psyr_1833 0.18 -0.5 2,113,769 - Psyr_1833 0.18 -0.9 2,113,769 - Psyr_1833 0.18 -1.0 2,113,770 + Psyr_1833 0.18 -0.3 2,113,770 + Psyr_1833 0.18 +0.3 2,113,770 + Psyr_1833 0.18 +0.7 2,113,770 + Psyr_1833 0.18 +0.1 2,113,770 + Psyr_1833 0.18 -0.4 2,113,770 + Psyr_1833 0.18 +0.2 2,113,770 + Psyr_1833 0.18 +1.4 2,113,770 + Psyr_1833 0.18 +0.8 2,113,770 + Psyr_1833 0.18 +0.1 2,113,771 - Psyr_1833 0.18 -0.3 2,113,771 - Psyr_1833 0.18 -0.9 2,113,771 - Psyr_1833 0.18 -1.5 2,113,771 - Psyr_1833 0.18 +4.7 2,113,771 - Psyr_1833 0.18 +0.7 2,113,771 - Psyr_1833 0.18 +0.0 2,113,771 - Psyr_1833 0.18 -2.5 2,113,771 - Psyr_1833 0.18 +1.0 2,113,771 - Psyr_1833 0.18 -0.6 2,113,771 - Psyr_1833 0.18 -0.7 2,113,849 + Psyr_1833 0.28 +0.5 2,113,849 + Psyr_1833 0.28 +0.3 2,113,850 - Psyr_1833 0.28 -0.2 2,113,850 - Psyr_1833 0.28 +1.0 2,114,002 + -3.3 2,114,002 + Psyr_1833 0.46 -2.5 2,114,002 + Psyr_1833 0.46 +0.7 2,114,002 + Psyr_1833 0.46 -2.9 2,114,003 - Psyr_1833 0.46 +4.6 2,114,003 - Psyr_1833 0.46 +0.7 2,114,003 - Psyr_1833 0.46 -0.2 2,114,003 - Psyr_1833 0.46 -3.0 2,114,003 - Psyr_1833 0.46 -0.1 2,114,003 - Psyr_1833 0.46 -0.7 2,114,003 - Psyr_1833 0.46 +0.6 2,114,003 - Psyr_1833 0.46 -1.2 2,114,038 + Psyr_1833 0.50 +0.2 2,114,038 + Psyr_1833 0.50 -0.5 2,114,122 + Psyr_1833 0.60 +0.8 2,114,123 - Psyr_1833 0.60 -1.2 2,114,123 - Psyr_1833 0.60 -2.5 2,114,145 + Psyr_1833 0.63 +2.1 2,114,156 - Psyr_1833 0.64 -3.3 2,114,156 - Psyr_1833 0.64 -0.3 2,114,156 - Psyr_1833 0.64 -1.6 2,114,225 - Psyr_1833 0.72 +2.6 2,114,247 + Psyr_1833 0.75 +0.1 2,114,334 + Psyr_1833 0.85 +0.1 2,114,334 + Psyr_1833 0.85 +0.5 2,114,335 - Psyr_1833 0.85 -3.6 2,114,360 + Psyr_1833 0.88 -0.1
Or see this region's nucleotide sequence