Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1821 and Psyr_1822 are separated by 657 nucleotides Psyr_1822 and Psyr_1823 are separated by 5 nucleotides
Psyr_1821: Psyr_1821 - GCN5-related N-acetyltransferase, at 2,100,895 to 2,101,383
_1821
Psyr_1822: Psyr_1822 - MscS Mechanosensitive ion channel, at 2,102,041 to 2,103,165
_1822
Psyr_1823: Psyr_1823 - Twin-arginine translocation pathway signal, at 2,103,171 to 2,104,856
_1823
Position (kb)
2102
2103
2104 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2101.263 kb on + strand, within Psyr_1821 at 2101.277 kb on + strand, within Psyr_1821 at 2101.278 kb on - strand, within Psyr_1821 at 2101.278 kb on - strand, within Psyr_1821 at 2101.297 kb on + strand, within Psyr_1821 at 2101.297 kb on + strand, within Psyr_1821 at 2101.298 kb on - strand, within Psyr_1821 at 2101.298 kb on - strand, within Psyr_1821 at 2101.298 kb on - strand, within Psyr_1821 at 2101.298 kb on - strand, within Psyr_1821 at 2101.312 kb on - strand, within Psyr_1821 at 2101.764 kb on - strand at 2101.773 kb on + strand at 2101.812 kb on + strand at 2101.812 kb on + strand at 2101.812 kb on + strand at 2101.812 kb on + strand at 2101.813 kb on - strand at 2101.873 kb on - strand at 2101.873 kb on - strand at 2101.873 kb on - strand at 2101.930 kb on - strand at 2102.219 kb on - strand, within Psyr_1822 at 2102.219 kb on - strand, within Psyr_1822 at 2102.219 kb on - strand, within Psyr_1822 at 2102.250 kb on + strand, within Psyr_1822 at 2102.250 kb on + strand, within Psyr_1822 at 2102.250 kb on + strand, within Psyr_1822 at 2102.251 kb on - strand, within Psyr_1822 at 2102.253 kb on + strand, within Psyr_1822 at 2102.253 kb on + strand, within Psyr_1822 at 2102.254 kb on - strand, within Psyr_1822 at 2102.254 kb on - strand, within Psyr_1822 at 2102.254 kb on - strand, within Psyr_1822 at 2102.254 kb on - strand, within Psyr_1822 at 2102.254 kb on - strand, within Psyr_1822 at 2102.254 kb on - strand, within Psyr_1822 at 2102.254 kb on - strand, within Psyr_1822 at 2102.408 kb on - strand, within Psyr_1822 at 2102.535 kb on + strand, within Psyr_1822 at 2102.535 kb on + strand, within Psyr_1822 at 2102.536 kb on - strand, within Psyr_1822 at 2102.791 kb on + strand, within Psyr_1822 at 2102.792 kb on - strand, within Psyr_1822 at 2102.839 kb on + strand, within Psyr_1822 at 2102.839 kb on + strand, within Psyr_1822 at 2102.839 kb on + strand, within Psyr_1822 at 2102.839 kb on + strand, within Psyr_1822 at 2102.839 kb on + strand, within Psyr_1822 at 2102.839 kb on + strand, within Psyr_1822 at 2102.839 kb on + strand, within Psyr_1822 at 2102.840 kb on - strand, within Psyr_1822 at 2102.840 kb on - strand, within Psyr_1822 at 2102.840 kb on - strand, within Psyr_1822 at 2102.840 kb on - strand, within Psyr_1822 at 2102.862 kb on - strand, within Psyr_1822 at 2102.995 kb on + strand, within Psyr_1822 at 2102.996 kb on - strand, within Psyr_1822 at 2102.996 kb on - strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.005 kb on - strand, within Psyr_1822 at 2103.005 kb on - strand, within Psyr_1822 at 2103.005 kb on - strand, within Psyr_1822 at 2103.005 kb on - strand, within Psyr_1822 at 2103.005 kb on - strand, within Psyr_1822 at 2103.006 kb on + strand, within Psyr_1822 at 2103.094 kb on + strand at 2103.095 kb on - strand at 2103.117 kb on + strand at 2103.577 kb on + strand, within Psyr_1823 at 2103.577 kb on + strand, within Psyr_1823 at 2103.577 kb on + strand, within Psyr_1823 at 2103.577 kb on + strand, within Psyr_1823 at 2103.577 kb on + strand, within Psyr_1823 at 2103.578 kb on - strand, within Psyr_1823 at 2103.712 kb on + strand, within Psyr_1823 at 2103.712 kb on + strand, within Psyr_1823 at 2103.713 kb on - strand, within Psyr_1823 at 2103.734 kb on - strand, within Psyr_1823 at 2103.967 kb on + strand, within Psyr_1823 at 2103.968 kb on - strand, within Psyr_1823 at 2103.968 kb on - strand, within Psyr_1823 at 2103.968 kb on - strand, within Psyr_1823
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 2,101,263 + Psyr_1821 0.75 +0.5 2,101,277 + Psyr_1821 0.78 -0.2 2,101,278 - Psyr_1821 0.78 -0.4 2,101,278 - Psyr_1821 0.78 +0.5 2,101,297 + Psyr_1821 0.82 +0.4 2,101,297 + Psyr_1821 0.82 +0.1 2,101,298 - Psyr_1821 0.82 -2.3 2,101,298 - Psyr_1821 0.82 +0.3 2,101,298 - Psyr_1821 0.82 +0.1 2,101,298 - Psyr_1821 0.82 +0.7 2,101,312 - Psyr_1821 0.85 +1.9 2,101,764 - -0.5 2,101,773 + -2.5 2,101,812 + -1.0 2,101,812 + -0.8 2,101,812 + +0.5 2,101,812 + +2.1 2,101,813 - -0.5 2,101,873 - +0.5 2,101,873 - +3.5 2,101,873 - -0.2 2,101,930 - +1.5 2,102,219 - Psyr_1822 0.16 -0.2 2,102,219 - Psyr_1822 0.16 +1.0 2,102,219 - Psyr_1822 0.16 -0.2 2,102,250 + Psyr_1822 0.19 -1.3 2,102,250 + Psyr_1822 0.19 -1.2 2,102,250 + Psyr_1822 0.19 -0.4 2,102,251 - Psyr_1822 0.19 +0.5 2,102,253 + Psyr_1822 0.19 +0.5 2,102,253 + Psyr_1822 0.19 +0.3 2,102,254 - Psyr_1822 0.19 +0.3 2,102,254 - Psyr_1822 0.19 +1.0 2,102,254 - Psyr_1822 0.19 +0.4 2,102,254 - Psyr_1822 0.19 -1.3 2,102,254 - Psyr_1822 0.19 +0.3 2,102,254 - Psyr_1822 0.19 +1.1 2,102,254 - Psyr_1822 0.19 -1.5 2,102,408 - Psyr_1822 0.33 -2.6 2,102,535 + Psyr_1822 0.44 +3.7 2,102,535 + Psyr_1822 0.44 +1.3 2,102,536 - Psyr_1822 0.44 +0.0 2,102,791 + Psyr_1822 0.67 +0.1 2,102,792 - Psyr_1822 0.67 +0.8 2,102,839 + Psyr_1822 0.71 +0.3 2,102,839 + Psyr_1822 0.71 +0.8 2,102,839 + Psyr_1822 0.71 +1.3 2,102,839 + Psyr_1822 0.71 -0.8 2,102,839 + Psyr_1822 0.71 +0.8 2,102,839 + Psyr_1822 0.71 +1.6 2,102,839 + Psyr_1822 0.71 -0.8 2,102,840 - Psyr_1822 0.71 +0.9 2,102,840 - Psyr_1822 0.71 +0.5 2,102,840 - Psyr_1822 0.71 +0.3 2,102,840 - Psyr_1822 0.71 +0.7 2,102,862 - Psyr_1822 0.73 -0.4 2,102,995 + Psyr_1822 0.85 +0.5 2,102,996 - Psyr_1822 0.85 -0.5 2,102,996 - Psyr_1822 0.85 +1.3 2,103,004 + Psyr_1822 0.86 -1.1 2,103,004 + Psyr_1822 0.86 +0.2 2,103,004 + Psyr_1822 0.86 +0.5 2,103,004 + Psyr_1822 0.86 +1.5 2,103,004 + Psyr_1822 0.86 +2.4 2,103,004 + Psyr_1822 0.86 +0.7 2,103,004 + Psyr_1822 0.86 +0.7 2,103,004 + Psyr_1822 0.86 +0.6 2,103,004 + Psyr_1822 0.86 +1.0 2,103,005 - Psyr_1822 0.86 +0.9 2,103,005 - Psyr_1822 0.86 +0.2 2,103,005 - Psyr_1822 0.86 -2.7 2,103,005 - Psyr_1822 0.86 -0.3 2,103,005 - Psyr_1822 0.86 +2.3 2,103,006 + Psyr_1822 0.86 +0.3 2,103,094 + -0.1 2,103,095 - +0.8 2,103,117 + +0.4 2,103,577 + Psyr_1823 0.24 +0.8 2,103,577 + Psyr_1823 0.24 +0.0 2,103,577 + Psyr_1823 0.24 -1.5 2,103,577 + Psyr_1823 0.24 +1.0 2,103,577 + Psyr_1823 0.24 -0.2 2,103,578 - Psyr_1823 0.24 +1.7 2,103,712 + Psyr_1823 0.32 -2.6 2,103,712 + Psyr_1823 0.32 -1.6 2,103,713 - Psyr_1823 0.32 +1.9 2,103,734 - Psyr_1823 0.33 +1.0 2,103,967 + Psyr_1823 0.47 +0.7 2,103,968 - Psyr_1823 0.47 +0.1 2,103,968 - Psyr_1823 0.47 +0.7 2,103,968 - Psyr_1823 0.47 +0.1
Or see this region's nucleotide sequence