Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1387 and Psyr_1388 are separated by 96 nucleotides Psyr_1388 and Psyr_1389 are separated by 10 nucleotides Psyr_1389 and Psyr_1390 are separated by 79 nucleotides Psyr_1390 and Psyr_1391 are separated by 92 nucleotides
Psyr_1387: Psyr_1387 - Ferredoxin--NADP(+) reductase, at 1,569,129 to 1,569,908
_1387
Psyr_1388: Psyr_1388 - Protein of unknown function UPF0066, at 1,570,005 to 1,570,697
_1388
Psyr_1389: Psyr_1389 - Protein of unknown function DUF1456, at 1,570,708 to 1,571,169
_1389
Psyr_1390: Psyr_1390 - RNA-binding S4, at 1,571,249 to 1,571,959
_1390
Psyr_1391: Psyr_1391 - GCN5-related N-acetyltransferase, at 1,572,052 to 1,572,492
_1391
Position (kb)
1570
1571
1572 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1569.906 kb on + strand at 1569.906 kb on + strand at 1569.907 kb on - strand at 1569.907 kb on - strand at 1569.907 kb on - strand at 1570.090 kb on + strand, within Psyr_1388 at 1570.300 kb on + strand, within Psyr_1388 at 1570.300 kb on + strand, within Psyr_1388 at 1570.300 kb on + strand, within Psyr_1388 at 1570.300 kb on + strand, within Psyr_1388 at 1570.300 kb on + strand, within Psyr_1388 at 1570.300 kb on + strand, within Psyr_1388 at 1570.301 kb on - strand, within Psyr_1388 at 1570.301 kb on - strand, within Psyr_1388 at 1570.301 kb on - strand, within Psyr_1388 at 1570.301 kb on - strand, within Psyr_1388 at 1570.301 kb on - strand, within Psyr_1388 at 1570.690 kb on + strand at 1570.690 kb on + strand at 1570.690 kb on + strand at 1570.690 kb on + strand at 1570.690 kb on + strand at 1570.690 kb on + strand at 1570.691 kb on - strand at 1570.691 kb on - strand at 1570.691 kb on - strand at 1570.691 kb on - strand at 1570.691 kb on - strand at 1570.691 kb on - strand at 1570.691 kb on - strand at 1570.775 kb on + strand, within Psyr_1389 at 1570.909 kb on - strand, within Psyr_1389 at 1570.974 kb on - strand, within Psyr_1389 at 1570.974 kb on - strand, within Psyr_1389 at 1570.974 kb on - strand, within Psyr_1389 at 1570.974 kb on - strand, within Psyr_1389 at 1570.974 kb on - strand, within Psyr_1389 at 1571.024 kb on + strand, within Psyr_1389 at 1571.024 kb on + strand, within Psyr_1389 at 1571.135 kb on + strand at 1571.135 kb on + strand at 1571.136 kb on - strand at 1571.136 kb on - strand at 1571.136 kb on - strand at 1571.152 kb on + strand at 1571.152 kb on + strand at 1571.152 kb on + strand at 1571.152 kb on + strand at 1571.152 kb on + strand at 1571.152 kb on + strand at 1571.153 kb on - strand at 1571.153 kb on - strand at 1571.153 kb on - strand at 1571.186 kb on + strand at 1571.186 kb on + strand at 1571.186 kb on + strand at 1571.186 kb on + strand at 1571.187 kb on - strand at 1571.226 kb on + strand at 1571.227 kb on - strand at 1571.227 kb on - strand at 1571.227 kb on - strand at 1571.229 kb on + strand at 1571.229 kb on + strand at 1571.230 kb on - strand at 1571.274 kb on + strand at 1571.275 kb on - strand at 1571.275 kb on - strand at 1571.466 kb on + strand, within Psyr_1390 at 1571.466 kb on + strand, within Psyr_1390 at 1571.466 kb on + strand, within Psyr_1390 at 1571.466 kb on + strand, within Psyr_1390 at 1571.466 kb on + strand, within Psyr_1390 at 1571.467 kb on - strand, within Psyr_1390 at 1571.467 kb on - strand, within Psyr_1390 at 1571.467 kb on - strand, within Psyr_1390 at 1571.532 kb on + strand, within Psyr_1390 at 1571.532 kb on + strand, within Psyr_1390 at 1571.532 kb on + strand, within Psyr_1390 at 1571.532 kb on + strand, within Psyr_1390 at 1571.533 kb on - strand, within Psyr_1390 at 1571.533 kb on - strand, within Psyr_1390 at 1571.533 kb on - strand, within Psyr_1390 at 1571.533 kb on - strand, within Psyr_1390 at 1571.679 kb on + strand, within Psyr_1390 at 1571.680 kb on - strand, within Psyr_1390 at 1571.680 kb on - strand, within Psyr_1390 at 1571.691 kb on - strand, within Psyr_1390 at 1571.978 kb on + strand at 1571.978 kb on + strand at 1571.998 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 1,569,906 + -1.6 1,569,906 + +0.5 1,569,907 - +2.3 1,569,907 - -0.1 1,569,907 - -1.1 1,570,090 + Psyr_1388 0.12 +0.3 1,570,300 + Psyr_1388 0.43 -2.5 1,570,300 + Psyr_1388 0.43 -0.3 1,570,300 + Psyr_1388 0.43 +0.3 1,570,300 + Psyr_1388 0.43 -0.7 1,570,300 + Psyr_1388 0.43 +1.7 1,570,300 + Psyr_1388 0.43 +1.4 1,570,301 - Psyr_1388 0.43 -0.2 1,570,301 - Psyr_1388 0.43 -0.1 1,570,301 - Psyr_1388 0.43 +0.3 1,570,301 - Psyr_1388 0.43 +1.4 1,570,301 - Psyr_1388 0.43 +1.2 1,570,690 + -0.3 1,570,690 + -0.2 1,570,690 + +0.6 1,570,690 + -0.8 1,570,690 + -2.9 1,570,690 + +0.7 1,570,691 - -0.2 1,570,691 - -1.4 1,570,691 - -0.1 1,570,691 - +0.6 1,570,691 - +0.9 1,570,691 - +0.7 1,570,691 - -0.6 1,570,775 + Psyr_1389 0.15 +0.0 1,570,909 - Psyr_1389 0.44 -2.0 1,570,974 - Psyr_1389 0.58 +0.3 1,570,974 - Psyr_1389 0.58 -3.4 1,570,974 - Psyr_1389 0.58 -0.5 1,570,974 - Psyr_1389 0.58 -1.0 1,570,974 - Psyr_1389 0.58 -1.4 1,571,024 + Psyr_1389 0.68 -0.9 1,571,024 + Psyr_1389 0.68 +0.6 1,571,135 + -0.3 1,571,135 + +1.3 1,571,136 - -1.1 1,571,136 - -1.2 1,571,136 - +0.2 1,571,152 + -0.1 1,571,152 + +0.0 1,571,152 + -1.0 1,571,152 + +0.4 1,571,152 + +0.3 1,571,152 + +1.3 1,571,153 - +0.4 1,571,153 - -1.0 1,571,153 - -1.0 1,571,186 + +1.0 1,571,186 + -1.1 1,571,186 + +0.6 1,571,186 + +0.5 1,571,187 - -0.7 1,571,226 + -1.0 1,571,227 - -0.4 1,571,227 - -1.4 1,571,227 - +0.1 1,571,229 + -2.4 1,571,229 + +2.6 1,571,230 - -1.9 1,571,274 + +0.2 1,571,275 - +0.8 1,571,275 - +1.4 1,571,466 + Psyr_1390 0.31 +2.1 1,571,466 + Psyr_1390 0.31 -1.8 1,571,466 + Psyr_1390 0.31 -0.2 1,571,466 + Psyr_1390 0.31 -0.7 1,571,466 + Psyr_1390 0.31 -0.8 1,571,467 - Psyr_1390 0.31 -2.0 1,571,467 - Psyr_1390 0.31 -0.4 1,571,467 - Psyr_1390 0.31 -0.1 1,571,532 + Psyr_1390 0.40 -1.8 1,571,532 + Psyr_1390 0.40 -2.6 1,571,532 + Psyr_1390 0.40 -0.1 1,571,532 + Psyr_1390 0.40 -1.0 1,571,533 - Psyr_1390 0.40 -0.0 1,571,533 - Psyr_1390 0.40 -0.4 1,571,533 - Psyr_1390 0.40 -0.6 1,571,533 - Psyr_1390 0.40 +0.3 1,571,679 + Psyr_1390 0.60 +0.8 1,571,680 - Psyr_1390 0.61 +1.8 1,571,680 - Psyr_1390 0.61 -0.7 1,571,691 - Psyr_1390 0.62 -0.4 1,571,978 + +0.2 1,571,978 + +0.8 1,571,998 - -0.7
Or see this region's nucleotide sequence