Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0952 and Psyr_0953 are separated by 118 nucleotides Psyr_0953 and Psyr_0954 are separated by 302 nucleotides Psyr_0954 and Psyr_0955 are separated by 86 nucleotides Psyr_0955 and Psyr_0956 overlap by 8 nucleotides
Psyr_0952: Psyr_0952 - glutamate racemase, at 1,087,574 to 1,088,389
_0952
Psyr_0953: Psyr_0953 - conserved hypothetical protein, at 1,088,508 to 1,089,026
_0953
Psyr_0954: Psyr_0954 - Protein of unknown function UPF0153, at 1,089,329 to 1,089,553
_0954
Psyr_0955: Psyr_0955 - membrane protein, putative, at 1,089,640 to 1,090,149
_0955
Psyr_0956: Psyr_0956 - Cyclopropane-fatty-acyl-phospholipid synthase, at 1,090,142 to 1,091,410
_0956
Position (kb) 1089
1090 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 1088.424 kb on + strand at 1088.424 kb on + strand at 1088.425 kb on - strand at 1088.425 kb on - strand at 1088.425 kb on - strand at 1088.445 kb on + strand at 1088.445 kb on + strand at 1088.446 kb on - strand at 1088.446 kb on - strand at 1088.446 kb on - strand at 1088.446 kb on - strand at 1088.446 kb on - strand at 1088.447 kb on + strand at 1088.447 kb on + strand at 1088.447 kb on + strand at 1088.486 kb on + strand at 1088.491 kb on + strand at 1088.491 kb on + strand at 1088.491 kb on + strand at 1088.491 kb on + strand at 1088.492 kb on - strand at 1088.492 kb on - strand at 1088.492 kb on - strand at 1088.492 kb on - strand at 1088.495 kb on + strand at 1088.496 kb on - strand at 1088.496 kb on - strand at 1088.625 kb on + strand, within Psyr_0953 at 1088.625 kb on + strand, within Psyr_0953 at 1088.626 kb on - strand, within Psyr_0953 at 1088.654 kb on + strand, within Psyr_0953 at 1088.709 kb on + strand, within Psyr_0953 at 1088.709 kb on + strand, within Psyr_0953 at 1088.709 kb on + strand, within Psyr_0953 at 1088.709 kb on + strand, within Psyr_0953 at 1088.709 kb on + strand, within Psyr_0953 at 1088.710 kb on - strand, within Psyr_0953 at 1088.710 kb on - strand, within Psyr_0953 at 1088.710 kb on - strand, within Psyr_0953 at 1088.715 kb on + strand, within Psyr_0953 at 1088.715 kb on + strand, within Psyr_0953 at 1088.716 kb on - strand, within Psyr_0953 at 1088.733 kb on + strand, within Psyr_0953 at 1088.733 kb on + strand, within Psyr_0953 at 1088.733 kb on + strand, within Psyr_0953 at 1088.733 kb on + strand, within Psyr_0953 at 1088.734 kb on - strand, within Psyr_0953 at 1088.734 kb on - strand, within Psyr_0953 at 1088.742 kb on - strand, within Psyr_0953 at 1088.776 kb on - strand, within Psyr_0953 at 1088.805 kb on + strand, within Psyr_0953 at 1088.805 kb on + strand, within Psyr_0953 at 1088.805 kb on + strand, within Psyr_0953 at 1088.805 kb on + strand, within Psyr_0953 at 1088.809 kb on + strand, within Psyr_0953 at 1088.809 kb on + strand, within Psyr_0953 at 1088.809 kb on + strand, within Psyr_0953 at 1088.809 kb on + strand, within Psyr_0953 at 1088.809 kb on + strand, within Psyr_0953 at 1088.809 kb on + strand, within Psyr_0953 at 1088.809 kb on + strand, within Psyr_0953 at 1088.809 kb on + strand, within Psyr_0953 at 1088.809 kb on + strand, within Psyr_0953 at 1088.809 kb on + strand, within Psyr_0953 at 1088.809 kb on + strand, within Psyr_0953 at 1088.809 kb on + strand, within Psyr_0953 at 1088.810 kb on - strand, within Psyr_0953 at 1088.810 kb on - strand, within Psyr_0953 at 1088.810 kb on - strand, within Psyr_0953 at 1088.810 kb on - strand, within Psyr_0953 at 1088.810 kb on - strand, within Psyr_0953 at 1088.810 kb on - strand, within Psyr_0953 at 1088.810 kb on - strand, within Psyr_0953 at 1088.810 kb on - strand, within Psyr_0953 at 1088.810 kb on - strand, within Psyr_0953 at 1088.989 kb on + strand at 1088.989 kb on + strand at 1088.989 kb on + strand at 1088.989 kb on + strand at 1088.989 kb on + strand at 1088.989 kb on + strand at 1088.989 kb on + strand at 1088.989 kb on + strand at 1088.989 kb on + strand at 1088.989 kb on + strand at 1088.989 kb on + strand at 1088.990 kb on - strand at 1088.990 kb on - strand at 1088.990 kb on - strand at 1088.990 kb on - strand at 1088.990 kb on - strand at 1088.990 kb on - strand at 1088.990 kb on - strand at 1088.998 kb on - strand at 1089.009 kb on + strand at 1089.009 kb on + strand at 1089.009 kb on + strand at 1089.009 kb on + strand at 1089.009 kb on + strand at 1089.010 kb on - strand at 1089.183 kb on + strand at 1089.184 kb on - strand at 1089.185 kb on + strand at 1089.185 kb on + strand at 1089.185 kb on + strand at 1089.186 kb on - strand at 1089.186 kb on - strand at 1089.186 kb on - strand at 1089.255 kb on - strand at 1089.256 kb on - strand at 1089.289 kb on + strand at 1089.289 kb on + strand at 1089.289 kb on + strand at 1089.289 kb on + strand at 1089.289 kb on + strand at 1089.290 kb on - strand at 1089.290 kb on - strand at 1089.290 kb on - strand at 1089.291 kb on + strand at 1089.298 kb on + strand at 1089.298 kb on + strand at 1089.336 kb on + strand at 1089.336 kb on + strand at 1089.336 kb on + strand at 1089.337 kb on - strand at 1089.516 kb on + strand, within Psyr_0954 at 1089.516 kb on + strand, within Psyr_0954 at 1089.516 kb on + strand, within Psyr_0954 at 1089.517 kb on - strand, within Psyr_0954 at 1089.517 kb on - strand, within Psyr_0954 at 1089.635 kb on - strand at 1089.635 kb on - strand at 1089.700 kb on + strand, within Psyr_0955 at 1089.700 kb on + strand, within Psyr_0955 at 1089.700 kb on + strand, within Psyr_0955 at 1089.700 kb on + strand, within Psyr_0955 at 1089.700 kb on + strand, within Psyr_0955 at 1089.700 kb on + strand, within Psyr_0955 at 1089.700 kb on + strand, within Psyr_0955 at 1089.700 kb on + strand, within Psyr_0955 at 1089.700 kb on + strand, within Psyr_0955 at 1089.701 kb on - strand, within Psyr_0955 at 1089.701 kb on - strand, within Psyr_0955 at 1089.786 kb on - strand, within Psyr_0955 at 1089.786 kb on - strand, within Psyr_0955 at 1089.786 kb on - strand, within Psyr_0955 at 1089.786 kb on - strand, within Psyr_0955 at 1089.786 kb on - strand, within Psyr_0955 at 1089.788 kb on + strand, within Psyr_0955 at 1089.788 kb on + strand, within Psyr_0955 at 1089.788 kb on + strand, within Psyr_0955 at 1089.788 kb on + strand, within Psyr_0955 at 1089.789 kb on - strand, within Psyr_0955 at 1089.960 kb on + strand, within Psyr_0955 at 1089.960 kb on + strand, within Psyr_0955 at 1089.960 kb on + strand, within Psyr_0955 at 1089.960 kb on + strand, within Psyr_0955 at 1090.143 kb on + strand at 1090.143 kb on + strand at 1090.246 kb on - strand at 1090.254 kb on + strand at 1090.254 kb on + strand at 1090.287 kb on + strand, within Psyr_0956 at 1090.288 kb on - strand, within Psyr_0956 at 1090.461 kb on + strand, within Psyr_0956
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 1,088,424 + +0.4 1,088,424 + -1.6 1,088,425 - -0.4 1,088,425 - +0.2 1,088,425 - -0.6 1,088,445 + +0.7 1,088,445 + -2.1 1,088,446 - -0.6 1,088,446 - +1.4 1,088,446 - -0.9 1,088,446 - -0.3 1,088,446 - -0.4 1,088,447 + -1.2 1,088,447 + +0.6 1,088,447 + +0.8 1,088,486 + -1.4 1,088,491 + -0.9 1,088,491 + +0.0 1,088,491 + -1.3 1,088,491 + +0.2 1,088,492 - +0.1 1,088,492 - -0.1 1,088,492 - -2.4 1,088,492 - -0.2 1,088,495 + +0.2 1,088,496 - -0.3 1,088,496 - -0.5 1,088,625 + Psyr_0953 0.23 +0.4 1,088,625 + Psyr_0953 0.23 +0.6 1,088,626 - Psyr_0953 0.23 -0.6 1,088,654 + Psyr_0953 0.28 -0.9 1,088,709 + Psyr_0953 0.39 +0.4 1,088,709 + Psyr_0953 0.39 +0.7 1,088,709 + Psyr_0953 0.39 +0.3 1,088,709 + Psyr_0953 0.39 -0.5 1,088,709 + Psyr_0953 0.39 +0.0 1,088,710 - Psyr_0953 0.39 -0.4 1,088,710 - Psyr_0953 0.39 +0.6 1,088,710 - Psyr_0953 0.39 -0.1 1,088,715 + Psyr_0953 0.40 -0.2 1,088,715 + Psyr_0953 0.40 +0.7 1,088,716 - Psyr_0953 0.40 -0.1 1,088,733 + Psyr_0953 0.43 +0.2 1,088,733 + Psyr_0953 0.43 -0.0 1,088,733 + Psyr_0953 0.43 +1.0 1,088,733 + Psyr_0953 0.43 +0.6 1,088,734 - Psyr_0953 0.44 -0.5 1,088,734 - Psyr_0953 0.44 -0.5 1,088,742 - Psyr_0953 0.45 -0.7 1,088,776 - Psyr_0953 0.52 -0.6 1,088,805 + Psyr_0953 0.57 +0.9 1,088,805 + Psyr_0953 0.57 +1.8 1,088,805 + Psyr_0953 0.57 +1.4 1,088,805 + Psyr_0953 0.57 +0.2 1,088,809 + Psyr_0953 0.58 -0.0 1,088,809 + Psyr_0953 0.58 -0.8 1,088,809 + Psyr_0953 0.58 +1.8 1,088,809 + Psyr_0953 0.58 +0.6 1,088,809 + Psyr_0953 0.58 -0.4 1,088,809 + Psyr_0953 0.58 +0.7 1,088,809 + Psyr_0953 0.58 -0.2 1,088,809 + Psyr_0953 0.58 +1.2 1,088,809 + Psyr_0953 0.58 -0.7 1,088,809 + Psyr_0953 0.58 +0.3 1,088,809 + Psyr_0953 0.58 +0.4 1,088,809 + Psyr_0953 0.58 +0.4 1,088,810 - Psyr_0953 0.58 -1.0 1,088,810 - Psyr_0953 0.58 -1.2 1,088,810 - Psyr_0953 0.58 -1.1 1,088,810 - Psyr_0953 0.58 -0.9 1,088,810 - Psyr_0953 0.58 -4.1 1,088,810 - Psyr_0953 0.58 +0.5 1,088,810 - Psyr_0953 0.58 -0.8 1,088,810 - Psyr_0953 0.58 +1.4 1,088,810 - Psyr_0953 0.58 -1.2 1,088,989 + -0.5 1,088,989 + -0.3 1,088,989 + -0.3 1,088,989 + +0.2 1,088,989 + -0.1 1,088,989 + -2.6 1,088,989 + +0.5 1,088,989 + +0.4 1,088,989 + +0.0 1,088,989 + +1.9 1,088,989 + -0.0 1,088,990 - -0.8 1,088,990 - -0.6 1,088,990 - -0.7 1,088,990 - -0.4 1,088,990 - -0.3 1,088,990 - -1.3 1,088,990 - -0.6 1,088,998 - -2.5 1,089,009 + +1.6 1,089,009 + -0.9 1,089,009 + -0.8 1,089,009 + +1.6 1,089,009 + +0.1 1,089,010 - -2.2 1,089,183 + -0.5 1,089,184 - +0.6 1,089,185 + +0.6 1,089,185 + +0.5 1,089,185 + -0.8 1,089,186 - -0.0 1,089,186 - +0.1 1,089,186 - +0.4 1,089,255 - +0.2 1,089,256 - -0.1 1,089,289 + -2.6 1,089,289 + -3.3 1,089,289 + -1.6 1,089,289 + -0.7 1,089,289 + -0.2 1,089,290 - -1.2 1,089,290 - +0.9 1,089,290 - +1.8 1,089,291 + -1.1 1,089,298 + -1.1 1,089,298 + +0.1 1,089,336 + -1.6 1,089,336 + -0.6 1,089,336 + -0.8 1,089,337 - -0.2 1,089,516 + Psyr_0954 0.83 -2.2 1,089,516 + Psyr_0954 0.83 -1.8 1,089,516 + Psyr_0954 0.83 -0.9 1,089,517 - Psyr_0954 0.84 +0.4 1,089,517 - Psyr_0954 0.84 -0.7 1,089,635 - +0.4 1,089,635 - -1.1 1,089,700 + Psyr_0955 0.12 +1.3 1,089,700 + Psyr_0955 0.12 -0.0 1,089,700 + Psyr_0955 0.12 -0.3 1,089,700 + Psyr_0955 0.12 -1.9 1,089,700 + Psyr_0955 0.12 +0.1 1,089,700 + Psyr_0955 0.12 -1.0 1,089,700 + Psyr_0955 0.12 -2.5 1,089,700 + Psyr_0955 0.12 -0.2 1,089,700 + Psyr_0955 0.12 +2.4 1,089,701 - Psyr_0955 0.12 +0.5 1,089,701 - Psyr_0955 0.12 +1.1 1,089,786 - Psyr_0955 0.29 -0.0 1,089,786 - Psyr_0955 0.29 +1.2 1,089,786 - Psyr_0955 0.29 +0.3 1,089,786 - Psyr_0955 0.29 -1.2 1,089,786 - Psyr_0955 0.29 +0.0 1,089,788 + Psyr_0955 0.29 +0.9 1,089,788 + Psyr_0955 0.29 +3.0 1,089,788 + Psyr_0955 0.29 +1.3 1,089,788 + Psyr_0955 0.29 -0.6 1,089,789 - Psyr_0955 0.29 +1.0 1,089,960 + Psyr_0955 0.63 -0.1 1,089,960 + Psyr_0955 0.63 +0.3 1,089,960 + Psyr_0955 0.63 -0.4 1,089,960 + Psyr_0955 0.63 +1.5 1,090,143 + +0.8 1,090,143 + -1.0 1,090,246 - -0.0 1,090,254 + +0.1 1,090,254 + +1.0 1,090,287 + Psyr_0956 0.11 +0.9 1,090,288 - Psyr_0956 0.12 -0.7 1,090,461 + Psyr_0956 0.25 +0.0
Or see this region's nucleotide sequence