Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0689 and Psyr_0690 are separated by 33 nucleotides Psyr_0690 and Psyr_0691 are separated by 204 nucleotides
Psyr_0689: Psyr_0689 - methylmalonate-semialdehyde dehydrogenase (acylating), at 792,585 to 794,111
_0689
Psyr_0690: Psyr_0690 - 3-hydroxyisobutyrate dehydrogenase, at 794,145 to 795,032
_0690
Psyr_0691: Psyr_0691 - gamma-glutamyltransferase 1, Threonine peptidase, MEROPS family T03, at 795,237 to 796,964
_0691
Position (kb)
794
795
796 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 793.317 kb on + strand, within Psyr_0689 at 793.317 kb on + strand, within Psyr_0689 at 793.387 kb on + strand, within Psyr_0689 at 793.427 kb on - strand, within Psyr_0689 at 793.749 kb on + strand, within Psyr_0689 at 793.749 kb on + strand, within Psyr_0689 at 793.749 kb on + strand, within Psyr_0689 at 793.749 kb on + strand, within Psyr_0689 at 793.749 kb on + strand, within Psyr_0689 at 793.749 kb on + strand, within Psyr_0689 at 793.750 kb on - strand, within Psyr_0689 at 793.750 kb on - strand, within Psyr_0689 at 793.750 kb on - strand, within Psyr_0689 at 793.925 kb on + strand, within Psyr_0689 at 793.926 kb on - strand, within Psyr_0689 at 794.002 kb on - strand at 794.025 kb on + strand at 794.025 kb on + strand at 794.025 kb on + strand at 794.025 kb on + strand at 794.025 kb on + strand at 794.025 kb on + strand at 794.025 kb on + strand at 794.026 kb on - strand at 794.026 kb on - strand at 794.026 kb on - strand at 794.026 kb on - strand at 794.026 kb on - strand at 794.026 kb on - strand at 794.026 kb on - strand at 794.174 kb on + strand at 794.365 kb on - strand, within Psyr_0690 at 794.762 kb on + strand, within Psyr_0690 at 794.763 kb on - strand, within Psyr_0690 at 794.763 kb on - strand, within Psyr_0690 at 794.796 kb on + strand, within Psyr_0690 at 794.797 kb on - strand, within Psyr_0690 at 794.797 kb on - strand, within Psyr_0690 at 794.797 kb on - strand, within Psyr_0690 at 794.797 kb on - strand, within Psyr_0690 at 794.797 kb on - strand, within Psyr_0690 at 794.797 kb on - strand, within Psyr_0690 at 794.955 kb on + strand at 794.956 kb on - strand at 795.030 kb on + strand at 795.030 kb on + strand at 795.031 kb on - strand at 795.031 kb on - strand at 795.031 kb on - strand at 795.031 kb on - strand at 795.031 kb on - strand at 795.031 kb on - strand at 795.291 kb on + strand at 795.292 kb on - strand at 795.388 kb on + strand at 795.388 kb on + strand at 795.388 kb on + strand at 795.388 kb on + strand at 795.388 kb on + strand at 795.389 kb on - strand at 795.389 kb on - strand at 795.389 kb on - strand at 795.389 kb on - strand at 795.389 kb on - strand at 795.389 kb on - strand at 795.405 kb on - strand at 795.570 kb on + strand, within Psyr_0691 at 795.570 kb on + strand, within Psyr_0691 at 795.570 kb on + strand, within Psyr_0691 at 795.570 kb on + strand, within Psyr_0691 at 795.570 kb on + strand, within Psyr_0691 at 795.570 kb on + strand, within Psyr_0691 at 795.570 kb on + strand, within Psyr_0691 at 795.570 kb on + strand, within Psyr_0691 at 795.570 kb on + strand, within Psyr_0691 at 795.570 kb on + strand, within Psyr_0691 at 795.570 kb on + strand, within Psyr_0691 at 795.571 kb on - strand, within Psyr_0691 at 795.571 kb on - strand, within Psyr_0691 at 795.571 kb on - strand, within Psyr_0691 at 795.571 kb on - strand, within Psyr_0691 at 795.571 kb on - strand, within Psyr_0691 at 795.571 kb on - strand, within Psyr_0691 at 795.571 kb on - strand, within Psyr_0691 at 795.675 kb on + strand, within Psyr_0691 at 795.676 kb on - strand, within Psyr_0691 at 795.676 kb on - strand, within Psyr_0691 at 795.676 kb on - strand, within Psyr_0691 at 795.676 kb on - strand, within Psyr_0691 at 795.739 kb on + strand, within Psyr_0691 at 795.739 kb on + strand, within Psyr_0691 at 795.739 kb on + strand, within Psyr_0691 at 795.740 kb on - strand, within Psyr_0691 at 795.761 kb on + strand, within Psyr_0691 at 795.761 kb on + strand, within Psyr_0691 at 795.921 kb on + strand, within Psyr_0691 at 795.922 kb on - strand, within Psyr_0691 at 795.922 kb on - strand, within Psyr_0691 at 795.922 kb on - strand, within Psyr_0691 at 796.002 kb on + strand, within Psyr_0691 at 796.002 kb on + strand, within Psyr_0691 at 796.003 kb on - strand, within Psyr_0691 at 796.003 kb on - strand, within Psyr_0691 at 796.003 kb on - strand, within Psyr_0691 at 796.003 kb on - strand, within Psyr_0691 at 796.003 kb on - strand, within Psyr_0691
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 793,317 + Psyr_0689 0.48 +0.6 793,317 + Psyr_0689 0.48 -1.1 793,387 + Psyr_0689 0.53 +0.5 793,427 - Psyr_0689 0.55 +0.1 793,749 + Psyr_0689 0.76 -0.2 793,749 + Psyr_0689 0.76 +0.1 793,749 + Psyr_0689 0.76 -0.8 793,749 + Psyr_0689 0.76 +1.5 793,749 + Psyr_0689 0.76 +0.2 793,749 + Psyr_0689 0.76 +0.4 793,750 - Psyr_0689 0.76 -0.8 793,750 - Psyr_0689 0.76 -0.0 793,750 - Psyr_0689 0.76 -1.1 793,925 + Psyr_0689 0.88 +2.4 793,926 - Psyr_0689 0.88 -0.0 794,002 - -1.1 794,025 + +0.3 794,025 + -0.1 794,025 + -0.2 794,025 + -0.6 794,025 + +0.5 794,025 + +0.2 794,025 + -0.1 794,026 - -0.9 794,026 - -2.6 794,026 - +0.1 794,026 - -1.5 794,026 - -0.8 794,026 - +0.5 794,026 - -0.7 794,174 + +0.6 794,365 - Psyr_0690 0.25 -0.0 794,762 + Psyr_0690 0.69 -0.5 794,763 - Psyr_0690 0.70 -1.6 794,763 - Psyr_0690 0.70 -2.3 794,796 + Psyr_0690 0.73 +0.4 794,797 - Psyr_0690 0.73 -0.2 794,797 - Psyr_0690 0.73 -1.3 794,797 - Psyr_0690 0.73 +0.7 794,797 - Psyr_0690 0.73 -1.2 794,797 - Psyr_0690 0.73 -0.7 794,797 - Psyr_0690 0.73 -0.3 794,955 + +1.2 794,956 - -1.1 795,030 + -0.3 795,030 + -0.7 795,031 - +3.2 795,031 - +0.4 795,031 - +0.4 795,031 - -0.8 795,031 - -1.8 795,031 - +1.2 795,291 + -0.4 795,292 - +0.7 795,388 + -0.0 795,388 + +0.5 795,388 + +1.0 795,388 + -0.3 795,388 + +0.4 795,389 - -0.6 795,389 - -1.6 795,389 - -1.6 795,389 - +0.8 795,389 - +0.4 795,389 - +1.2 795,405 - -0.9 795,570 + Psyr_0691 0.19 +1.0 795,570 + Psyr_0691 0.19 +0.5 795,570 + Psyr_0691 0.19 +0.7 795,570 + Psyr_0691 0.19 -1.4 795,570 + Psyr_0691 0.19 -0.8 795,570 + Psyr_0691 0.19 +1.0 795,570 + Psyr_0691 0.19 -0.6 795,570 + Psyr_0691 0.19 -0.5 795,570 + Psyr_0691 0.19 +0.0 795,570 + Psyr_0691 0.19 +1.0 795,570 + Psyr_0691 0.19 -0.5 795,571 - Psyr_0691 0.19 +1.1 795,571 - Psyr_0691 0.19 -0.4 795,571 - Psyr_0691 0.19 -1.2 795,571 - Psyr_0691 0.19 -1.0 795,571 - Psyr_0691 0.19 +0.1 795,571 - Psyr_0691 0.19 -0.5 795,571 - Psyr_0691 0.19 -0.5 795,675 + Psyr_0691 0.25 -1.0 795,676 - Psyr_0691 0.25 +0.3 795,676 - Psyr_0691 0.25 +0.1 795,676 - Psyr_0691 0.25 -0.9 795,676 - Psyr_0691 0.25 -3.4 795,739 + Psyr_0691 0.29 -2.0 795,739 + Psyr_0691 0.29 -3.9 795,739 + Psyr_0691 0.29 -0.8 795,740 - Psyr_0691 0.29 +0.5 795,761 + Psyr_0691 0.30 +0.0 795,761 + Psyr_0691 0.30 -0.4 795,921 + Psyr_0691 0.40 -0.4 795,922 - Psyr_0691 0.40 -0.3 795,922 - Psyr_0691 0.40 +1.0 795,922 - Psyr_0691 0.40 -1.2 796,002 + Psyr_0691 0.44 -1.1 796,002 + Psyr_0691 0.44 -0.2 796,003 - Psyr_0691 0.44 +0.3 796,003 - Psyr_0691 0.44 +0.3 796,003 - Psyr_0691 0.44 +1.4 796,003 - Psyr_0691 0.44 +3.1 796,003 - Psyr_0691 0.44 +0.3
Or see this region's nucleotide sequence