Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0635 and Psyr_0636 are separated by 203 nucleotides Psyr_0636 and Psyr_0637 overlap by 4 nucleotides Psyr_0637 and Psyr_0638 are separated by 14 nucleotides Psyr_0638 and Psyr_0639 are separated by 84 nucleotides
Psyr_0635: Psyr_0635 - MaoC-like dehydratase, at 725,613 to 726,068
_0635
Psyr_0636: Psyr_0636 - LrgA, at 726,272 to 726,634
_0636
Psyr_0637: Psyr_0637 - LrgB-like protein, at 726,631 to 727,347
_0637
Psyr_0638: Psyr_0638 - Peptidase S16, lon N-terminal, at 727,362 to 727,952
_0638
Psyr_0639: Psyr_0639 - lipoprotein, putative, at 728,037 to 728,603
_0639
Position (kb)
726
727
728 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 725.632 kb on - strand at 725.803 kb on + strand, within Psyr_0635 at 725.803 kb on + strand, within Psyr_0635 at 725.835 kb on + strand, within Psyr_0635 at 725.836 kb on - strand, within Psyr_0635 at 725.836 kb on - strand, within Psyr_0635 at 725.981 kb on + strand, within Psyr_0635 at 726.034 kb on + strand at 726.034 kb on + strand at 726.034 kb on + strand at 726.035 kb on - strand at 726.035 kb on - strand at 726.035 kb on - strand at 726.035 kb on - strand at 726.035 kb on - strand at 726.068 kb on + strand at 726.068 kb on + strand at 726.068 kb on + strand at 726.068 kb on + strand at 726.068 kb on + strand at 726.069 kb on - strand at 726.069 kb on - strand at 726.069 kb on - strand at 726.095 kb on + strand at 726.095 kb on + strand at 726.096 kb on - strand at 726.096 kb on - strand at 726.096 kb on - strand at 726.096 kb on - strand at 726.223 kb on + strand at 726.223 kb on + strand at 726.223 kb on + strand at 726.224 kb on - strand at 726.224 kb on - strand at 726.224 kb on - strand at 726.224 kb on - strand at 726.224 kb on - strand at 726.224 kb on - strand at 726.225 kb on + strand at 726.225 kb on + strand at 726.225 kb on + strand at 726.226 kb on - strand at 726.226 kb on - strand at 726.226 kb on - strand at 726.255 kb on + strand at 726.255 kb on + strand at 726.255 kb on + strand at 726.383 kb on - strand, within Psyr_0636 at 726.383 kb on - strand, within Psyr_0636 at 726.389 kb on + strand, within Psyr_0636 at 726.389 kb on + strand, within Psyr_0636 at 726.389 kb on + strand, within Psyr_0636 at 726.389 kb on + strand, within Psyr_0636 at 726.389 kb on + strand, within Psyr_0636 at 726.389 kb on + strand, within Psyr_0636 at 726.390 kb on - strand, within Psyr_0636 at 726.390 kb on - strand, within Psyr_0636 at 726.498 kb on - strand, within Psyr_0636 at 726.498 kb on - strand, within Psyr_0636 at 726.498 kb on - strand at 726.498 kb on - strand, within Psyr_0636 at 726.710 kb on - strand, within Psyr_0637 at 726.817 kb on + strand, within Psyr_0637 at 726.817 kb on + strand, within Psyr_0637 at 726.817 kb on + strand, within Psyr_0637 at 726.817 kb on + strand, within Psyr_0637 at 726.817 kb on + strand, within Psyr_0637 at 726.895 kb on + strand, within Psyr_0637 at 726.896 kb on - strand, within Psyr_0637 at 726.903 kb on + strand, within Psyr_0637 at 726.903 kb on + strand, within Psyr_0637 at 727.131 kb on - strand, within Psyr_0637 at 727.345 kb on + strand at 727.346 kb on - strand at 727.362 kb on - strand at 727.496 kb on + strand, within Psyr_0638 at 727.497 kb on - strand, within Psyr_0638 at 727.809 kb on + strand, within Psyr_0638 at 727.834 kb on - strand, within Psyr_0638 at 727.982 kb on + strand at 728.180 kb on + strand, within Psyr_0639 at 728.180 kb on + strand, within Psyr_0639 at 728.180 kb on + strand, within Psyr_0639 at 728.181 kb on - strand, within Psyr_0639 at 728.181 kb on - strand, within Psyr_0639 at 728.181 kb on - strand, within Psyr_0639 at 728.206 kb on + strand, within Psyr_0639 at 728.206 kb on + strand, within Psyr_0639 at 728.206 kb on - strand, within Psyr_0639 at 728.207 kb on - strand, within Psyr_0639 at 728.207 kb on - strand, within Psyr_0639 at 728.284 kb on + strand, within Psyr_0639 at 728.284 kb on + strand, within Psyr_0639 at 728.285 kb on - strand, within Psyr_0639
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 725,632 - -0.3 725,803 + Psyr_0635 0.42 +1.3 725,803 + Psyr_0635 0.42 -0.1 725,835 + Psyr_0635 0.49 +0.5 725,836 - Psyr_0635 0.49 -1.0 725,836 - Psyr_0635 0.49 +1.1 725,981 + Psyr_0635 0.81 -1.9 726,034 + -0.4 726,034 + +0.9 726,034 + +0.3 726,035 - -0.3 726,035 - -0.3 726,035 - +1.0 726,035 - +0.3 726,035 - +0.6 726,068 + +0.7 726,068 + -1.5 726,068 + +0.3 726,068 + +1.4 726,068 + +0.2 726,069 - -0.6 726,069 - +3.6 726,069 - -1.3 726,095 + +0.3 726,095 + -0.2 726,096 - -1.5 726,096 - -0.1 726,096 - +0.5 726,096 - -0.0 726,223 + -1.9 726,223 + +0.3 726,223 + -2.6 726,224 - -0.2 726,224 - -0.0 726,224 - -0.3 726,224 - +0.2 726,224 - -0.4 726,224 - -0.6 726,225 + -0.8 726,225 + +1.4 726,225 + -0.7 726,226 - -0.2 726,226 - -2.1 726,226 - -0.4 726,255 + +0.6 726,255 + +0.9 726,255 + +0.8 726,383 - Psyr_0636 0.31 -0.1 726,383 - Psyr_0636 0.31 +1.0 726,389 + Psyr_0636 0.32 +0.2 726,389 + Psyr_0636 0.32 +4.6 726,389 + Psyr_0636 0.32 +0.9 726,389 + Psyr_0636 0.32 -1.4 726,389 + Psyr_0636 0.32 -0.1 726,389 + Psyr_0636 0.32 +1.7 726,390 - Psyr_0636 0.33 +0.7 726,390 - Psyr_0636 0.33 -0.7 726,498 - Psyr_0636 0.62 -1.5 726,498 - Psyr_0636 0.62 +0.3 726,498 - -0.0 726,498 - Psyr_0636 0.62 -0.3 726,710 - Psyr_0637 0.11 +0.3 726,817 + Psyr_0637 0.26 +0.6 726,817 + Psyr_0637 0.26 +0.4 726,817 + Psyr_0637 0.26 +1.0 726,817 + Psyr_0637 0.26 +0.4 726,817 + Psyr_0637 0.26 +0.4 726,895 + Psyr_0637 0.37 +1.0 726,896 - Psyr_0637 0.37 -0.4 726,903 + Psyr_0637 0.38 +2.0 726,903 + Psyr_0637 0.38 +0.1 727,131 - Psyr_0637 0.70 -0.3 727,345 + +1.0 727,346 - +0.3 727,362 - -2.8 727,496 + Psyr_0638 0.23 -0.1 727,497 - Psyr_0638 0.23 -4.0 727,809 + Psyr_0638 0.76 -0.5 727,834 - Psyr_0638 0.80 +1.9 727,982 + -1.9 728,180 + Psyr_0639 0.25 -0.2 728,180 + Psyr_0639 0.25 -1.0 728,180 + Psyr_0639 0.25 +1.0 728,181 - Psyr_0639 0.25 -0.2 728,181 - Psyr_0639 0.25 -2.2 728,181 - Psyr_0639 0.25 -2.9 728,206 + Psyr_0639 0.30 +0.3 728,206 + Psyr_0639 0.30 -0.6 728,206 - Psyr_0639 0.30 +0.1 728,207 - Psyr_0639 0.30 +0.2 728,207 - Psyr_0639 0.30 -3.1 728,284 + Psyr_0639 0.44 -0.1 728,284 + Psyr_0639 0.44 -0.3 728,285 - Psyr_0639 0.44 +1.3
Or see this region's nucleotide sequence