Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0518 and Psyr_0519 are separated by 384 nucleotides Psyr_0519 and Psyr_0520 are separated by 129 nucleotides
Psyr_0518: Psyr_0518 - 2-oxo-acid dehydrogenase E1 component homodimeric type, at 576,281 to 578,926
_0518
Psyr_0519: Psyr_0519 - Glutamate-ammonia-ligase adenylyltransferase, at 579,311 to 582,268
_0519
Psyr_0520: Psyr_0520 - Glycosyl transferase, family 9, at 582,398 to 583,432
_0520
Position (kb) 579
580
581
582
583 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 579.001 kb on - strand at 579.001 kb on - strand at 579.137 kb on - strand at 579.142 kb on - strand at 579.142 kb on - strand at 579.318 kb on + strand at 579.319 kb on - strand at 579.556 kb on + strand at 579.556 kb on + strand at 579.557 kb on - strand at 579.557 kb on - strand at 579.639 kb on + strand, within Psyr_0519 at 579.640 kb on - strand, within Psyr_0519 at 579.640 kb on - strand, within Psyr_0519 at 579.762 kb on - strand, within Psyr_0519 at 579.762 kb on - strand, within Psyr_0519 at 579.773 kb on + strand, within Psyr_0519 at 579.773 kb on + strand, within Psyr_0519 at 579.773 kb on + strand, within Psyr_0519 at 579.773 kb on + strand, within Psyr_0519 at 579.774 kb on - strand, within Psyr_0519 at 580.106 kb on + strand, within Psyr_0519 at 580.106 kb on + strand, within Psyr_0519 at 580.107 kb on - strand, within Psyr_0519 at 580.109 kb on + strand, within Psyr_0519 at 580.109 kb on + strand, within Psyr_0519 at 580.109 kb on + strand, within Psyr_0519 at 580.452 kb on - strand, within Psyr_0519 at 580.506 kb on - strand, within Psyr_0519 at 580.506 kb on - strand, within Psyr_0519 at 580.637 kb on + strand, within Psyr_0519 at 580.637 kb on + strand, within Psyr_0519 at 580.637 kb on + strand, within Psyr_0519 at 580.637 kb on + strand, within Psyr_0519 at 580.637 kb on + strand, within Psyr_0519 at 580.638 kb on - strand, within Psyr_0519 at 580.638 kb on - strand, within Psyr_0519 at 580.638 kb on - strand, within Psyr_0519 at 580.638 kb on - strand, within Psyr_0519 at 580.638 kb on - strand, within Psyr_0519 at 580.638 kb on - strand, within Psyr_0519 at 580.638 kb on - strand, within Psyr_0519 at 580.638 kb on - strand, within Psyr_0519 at 580.987 kb on - strand, within Psyr_0519 at 580.987 kb on - strand, within Psyr_0519 at 581.012 kb on + strand, within Psyr_0519 at 581.012 kb on + strand, within Psyr_0519 at 581.019 kb on + strand, within Psyr_0519 at 581.020 kb on - strand, within Psyr_0519 at 581.203 kb on + strand, within Psyr_0519 at 581.324 kb on + strand, within Psyr_0519 at 581.325 kb on - strand, within Psyr_0519 at 581.456 kb on + strand, within Psyr_0519 at 581.639 kb on + strand, within Psyr_0519 at 581.639 kb on + strand, within Psyr_0519 at 581.640 kb on - strand, within Psyr_0519 at 582.011 kb on + strand at 582.056 kb on + strand at 582.056 kb on + strand at 582.140 kb on + strand at 582.140 kb on + strand at 582.140 kb on + strand at 582.140 kb on + strand at 582.140 kb on + strand at 582.140 kb on + strand at 582.140 kb on + strand at 582.140 kb on + strand at 582.140 kb on + strand at 582.142 kb on + strand at 582.142 kb on + strand at 582.304 kb on + strand at 582.304 kb on + strand at 582.304 kb on + strand at 582.304 kb on + strand at 582.304 kb on + strand at 582.304 kb on + strand at 582.304 kb on + strand at 582.304 kb on + strand at 582.304 kb on + strand at 582.306 kb on + strand at 582.312 kb on + strand at 582.312 kb on + strand at 582.312 kb on + strand at 582.312 kb on + strand at 582.312 kb on + strand at 582.312 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 579,001 - +0.0 579,001 - -2.9 579,137 - -2.4 579,142 - -4.0 579,142 - +0.7 579,318 + -2.4 579,319 - -0.4 579,556 + +1.8 579,556 + +0.1 579,557 - -2.8 579,557 - -0.4 579,639 + Psyr_0519 0.11 -1.4 579,640 - Psyr_0519 0.11 -1.1 579,640 - Psyr_0519 0.11 -3.6 579,762 - Psyr_0519 0.15 -0.6 579,762 - Psyr_0519 0.15 -0.3 579,773 + Psyr_0519 0.16 -1.0 579,773 + Psyr_0519 0.16 +0.6 579,773 + Psyr_0519 0.16 -0.2 579,773 + Psyr_0519 0.16 -1.7 579,774 - Psyr_0519 0.16 +0.4 580,106 + Psyr_0519 0.27 +0.1 580,106 + Psyr_0519 0.27 -2.4 580,107 - Psyr_0519 0.27 -0.5 580,109 + Psyr_0519 0.27 -0.6 580,109 + Psyr_0519 0.27 -0.3 580,109 + Psyr_0519 0.27 -0.1 580,452 - Psyr_0519 0.39 -0.2 580,506 - Psyr_0519 0.40 -0.5 580,506 - Psyr_0519 0.40 -2.4 580,637 + Psyr_0519 0.45 -0.4 580,637 + Psyr_0519 0.45 -0.6 580,637 + Psyr_0519 0.45 +1.1 580,637 + Psyr_0519 0.45 +0.6 580,637 + Psyr_0519 0.45 -0.6 580,638 - Psyr_0519 0.45 -0.6 580,638 - Psyr_0519 0.45 -2.9 580,638 - Psyr_0519 0.45 -1.2 580,638 - Psyr_0519 0.45 -3.3 580,638 - Psyr_0519 0.45 -0.5 580,638 - Psyr_0519 0.45 -1.3 580,638 - Psyr_0519 0.45 -0.6 580,638 - Psyr_0519 0.45 -1.9 580,987 - Psyr_0519 0.57 +0.3 580,987 - Psyr_0519 0.57 +1.1 581,012 + Psyr_0519 0.58 -1.9 581,012 + Psyr_0519 0.58 +0.7 581,019 + Psyr_0519 0.58 -0.6 581,020 - Psyr_0519 0.58 -0.4 581,203 + Psyr_0519 0.64 -0.4 581,324 + Psyr_0519 0.68 -0.3 581,325 - Psyr_0519 0.68 -0.3 581,456 + Psyr_0519 0.73 +0.1 581,639 + Psyr_0519 0.79 -0.3 581,639 + Psyr_0519 0.79 -2.8 581,640 - Psyr_0519 0.79 -0.6 582,011 + -1.9 582,056 + -1.4 582,056 + -0.5 582,140 + -0.1 582,140 + -0.1 582,140 + +0.4 582,140 + +0.1 582,140 + -1.2 582,140 + -0.1 582,140 + -1.7 582,140 + +1.7 582,140 + -0.9 582,142 + -1.4 582,142 + -0.4 582,304 + -0.5 582,304 + +0.3 582,304 + +1.2 582,304 + -0.6 582,304 + +0.3 582,304 + -1.9 582,304 + +0.1 582,304 + -0.5 582,304 + -0.6 582,306 + -1.4 582,312 + -1.6 582,312 + +0.4 582,312 + -0.4 582,312 + +0.4 582,312 + -0.2 582,312 + -0.0
Or see this region's nucleotide sequence