Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0458 and Psyr_0459 are separated by 311 nucleotides Psyr_0459 and Psyr_0460 are separated by 189 nucleotides
Psyr_0458: Psyr_0458 - Helicase, C-terminal:Type III restriction enzyme, res subunit:DEAD/DEAH box helicase, N-terminal, at 494,944 to 496,833
_0458
Psyr_0459: Psyr_0459 - 5,10-methylenetetrahydrofolate reductase, at 497,145 to 497,990
_0459
Psyr_0460: Psyr_0460 - adenosylhomocysteinase, at 498,180 to 499,589
_0460
Position (kb)
497
498 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 496.258 kb on - strand, within Psyr_0458 at 496.683 kb on + strand at 496.684 kb on - strand at 496.837 kb on + strand at 496.837 kb on + strand at 496.837 kb on + strand at 496.858 kb on + strand at 496.858 kb on + strand at 496.858 kb on + strand at 496.858 kb on + strand at 496.897 kb on - strand at 497.042 kb on + strand at 497.042 kb on + strand at 497.043 kb on - strand at 497.043 kb on - strand at 497.043 kb on - strand at 497.044 kb on + strand at 497.044 kb on + strand at 497.044 kb on + strand at 497.045 kb on - strand at 497.045 kb on - strand at 497.045 kb on - strand at 497.061 kb on + strand at 497.089 kb on + strand at 497.089 kb on + strand at 497.090 kb on - strand at 497.090 kb on - strand at 497.090 kb on - strand at 497.207 kb on - strand at 497.207 kb on - strand at 497.306 kb on - strand, within Psyr_0459 at 497.322 kb on - strand, within Psyr_0459 at 497.380 kb on + strand, within Psyr_0459 at 497.380 kb on + strand, within Psyr_0459 at 497.380 kb on + strand, within Psyr_0459 at 497.380 kb on + strand, within Psyr_0459 at 497.380 kb on + strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.479 kb on + strand, within Psyr_0459 at 497.479 kb on + strand, within Psyr_0459 at 497.479 kb on + strand, within Psyr_0459 at 497.480 kb on - strand, within Psyr_0459 at 497.480 kb on - strand, within Psyr_0459 at 497.728 kb on + strand, within Psyr_0459 at 497.729 kb on - strand, within Psyr_0459 at 497.729 kb on - strand, within Psyr_0459 at 497.769 kb on + strand, within Psyr_0459 at 497.866 kb on + strand, within Psyr_0459 at 497.866 kb on + strand, within Psyr_0459 at 497.866 kb on + strand, within Psyr_0459 at 497.866 kb on + strand, within Psyr_0459 at 497.866 kb on + strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.893 kb on + strand, within Psyr_0459 at 497.893 kb on + strand, within Psyr_0459 at 497.893 kb on + strand, within Psyr_0459 at 497.893 kb on + strand, within Psyr_0459 at 497.893 kb on + strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.951 kb on + strand at 498.013 kb on + strand at 498.014 kb on - strand at 498.014 kb on - strand at 498.014 kb on - strand at 498.024 kb on + strand at 498.025 kb on - strand at 498.044 kb on + strand at 498.045 kb on - strand at 498.045 kb on - strand at 498.087 kb on + strand at 498.088 kb on - strand at 498.088 kb on - strand at 498.088 kb on - strand at 498.088 kb on - strand at 498.088 kb on - strand at 498.174 kb on + strand at 498.174 kb on + strand at 498.174 kb on + strand at 498.174 kb on + strand at 498.174 kb on + strand at 498.174 kb on + strand at 498.175 kb on - strand at 498.175 kb on - strand at 498.175 kb on - strand at 498.175 kb on - strand at 498.178 kb on + strand at 498.178 kb on + strand at 498.178 kb on + strand at 498.178 kb on + strand at 498.179 kb on - strand at 498.179 kb on - strand at 498.179 kb on - strand at 498.179 kb on - strand at 498.179 kb on - strand at 498.179 kb on - strand at 498.179 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 496,258 - Psyr_0458 0.70 -0.5 496,683 + -0.1 496,684 - -0.4 496,837 + -1.4 496,837 + -0.4 496,837 + -0.2 496,858 + -1.2 496,858 + -0.4 496,858 + -0.1 496,858 + -0.7 496,897 - +0.6 497,042 + +0.2 497,042 + -2.9 497,043 - +0.3 497,043 - -0.4 497,043 - -1.1 497,044 + +0.1 497,044 + +0.2 497,044 + +0.9 497,045 - -1.3 497,045 - -0.3 497,045 - -0.6 497,061 + +2.0 497,089 + +0.6 497,089 + +1.0 497,090 - +0.1 497,090 - +1.0 497,090 - -1.6 497,207 - -0.7 497,207 - -0.3 497,306 - Psyr_0459 0.19 -0.6 497,322 - Psyr_0459 0.21 +0.3 497,380 + Psyr_0459 0.28 +0.6 497,380 + Psyr_0459 0.28 +1.3 497,380 + Psyr_0459 0.28 +0.9 497,380 + Psyr_0459 0.28 +0.9 497,380 + Psyr_0459 0.28 +0.5 497,381 - Psyr_0459 0.28 -1.8 497,381 - Psyr_0459 0.28 -1.6 497,381 - Psyr_0459 0.28 -0.5 497,381 - Psyr_0459 0.28 -0.3 497,381 - Psyr_0459 0.28 +2.0 497,458 + Psyr_0459 0.37 +0.6 497,458 + Psyr_0459 0.37 +0.4 497,458 + Psyr_0459 0.37 +0.2 497,458 + Psyr_0459 0.37 -0.9 497,458 + Psyr_0459 0.37 +1.3 497,458 + Psyr_0459 0.37 -3.4 497,458 + Psyr_0459 0.37 +0.3 497,458 + Psyr_0459 0.37 -0.0 497,458 + Psyr_0459 0.37 -1.8 497,459 - Psyr_0459 0.37 -0.6 497,459 - Psyr_0459 0.37 +2.2 497,459 - Psyr_0459 0.37 +1.5 497,479 + Psyr_0459 0.39 +0.8 497,479 + Psyr_0459 0.39 -0.2 497,479 + Psyr_0459 0.39 +1.6 497,480 - Psyr_0459 0.40 -0.7 497,480 - Psyr_0459 0.40 -0.3 497,728 + Psyr_0459 0.69 -0.4 497,729 - Psyr_0459 0.69 +0.8 497,729 - Psyr_0459 0.69 -0.6 497,769 + Psyr_0459 0.74 +0.4 497,866 + Psyr_0459 0.85 +0.5 497,866 + Psyr_0459 0.85 +0.0 497,866 + Psyr_0459 0.85 +0.3 497,866 + Psyr_0459 0.85 +0.7 497,866 + Psyr_0459 0.85 +0.1 497,867 - Psyr_0459 0.85 +0.5 497,867 - Psyr_0459 0.85 +1.0 497,867 - Psyr_0459 0.85 +0.7 497,867 - Psyr_0459 0.85 +1.4 497,867 - Psyr_0459 0.85 +0.8 497,867 - Psyr_0459 0.85 +0.3 497,893 + Psyr_0459 0.88 +0.9 497,893 + Psyr_0459 0.88 +0.5 497,893 + Psyr_0459 0.88 +0.3 497,893 + Psyr_0459 0.88 +0.2 497,893 + Psyr_0459 0.88 +0.7 497,894 - Psyr_0459 0.89 +0.4 497,894 - Psyr_0459 0.89 -0.0 497,894 - Psyr_0459 0.89 +4.4 497,894 - Psyr_0459 0.89 +1.4 497,894 - Psyr_0459 0.89 -1.6 497,894 - Psyr_0459 0.89 -1.8 497,894 - Psyr_0459 0.89 -0.8 497,951 + -0.1 498,013 + -1.5 498,014 - +0.8 498,014 - +0.1 498,014 - +0.0 498,024 + +1.0 498,025 - +0.2 498,044 + +0.7 498,045 - -1.5 498,045 - +0.9 498,087 + +0.7 498,088 - +0.3 498,088 - +0.2 498,088 - -0.5 498,088 - +0.5 498,088 - +1.8 498,174 + -1.3 498,174 + -0.7 498,174 + -0.4 498,174 + -0.5 498,174 + -0.0 498,174 + -0.7 498,175 - +0.2 498,175 - -0.8 498,175 - +0.9 498,175 - -0.5 498,178 + -0.6 498,178 + +1.4 498,178 + -0.9 498,178 + +1.6 498,179 - +0.7 498,179 - -0.5 498,179 - +0.6 498,179 - -0.4 498,179 - +0.1 498,179 - +1.0 498,179 - -0.4
Or see this region's nucleotide sequence