Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0408

Experiment: KB with Novobiocin 250 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0407 and Psyr_0408 are separated by 4 nucleotidesPsyr_0408 and Psyr_0409 are separated by 114 nucleotides Psyr_0407: Psyr_0407 - type II and III secretion system protein:NolW-like protein:NolW-like protein, at 436,404 to 438,560 _0407 Psyr_0408: Psyr_0408 - shikimate kinase, at 438,565 to 439,083 _0408 Psyr_0409: Psyr_0409 - 3-dehydroquinate synthase, at 439,198 to 440,301 _0409 Position (kb) 438 439 440Strain fitness (log2 ratio) -1 0 1 2 3at 437.765 kb on + strand, within Psyr_0407at 437.838 kb on + strand, within Psyr_0407at 437.839 kb on - strand, within Psyr_0407at 437.874 kb on - strand, within Psyr_0407at 437.907 kb on + strand, within Psyr_0407at 437.907 kb on + strand, within Psyr_0407at 437.908 kb on - strand, within Psyr_0407at 437.921 kb on + strand, within Psyr_0407at 438.294 kb on + strand, within Psyr_0407at 438.401 kb on + strandat 438.410 kb on + strandat 438.410 kb on + strandat 438.410 kb on + strandat 438.411 kb on - strandat 438.411 kb on - strandat 438.411 kb on - strandat 438.411 kb on - strandat 438.445 kb on + strandat 438.445 kb on + strandat 438.445 kb on + strandat 438.445 kb on + strandat 438.445 kb on + strandat 438.446 kb on - strandat 438.450 kb on + strandat 438.578 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml
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437,765 + Psyr_0407 0.63 -0.4
437,838 + Psyr_0407 0.66 +0.6
437,839 - Psyr_0407 0.67 +0.5
437,874 - Psyr_0407 0.68 -0.1
437,907 + Psyr_0407 0.70 -0.7
437,907 + Psyr_0407 0.70 +0.3
437,908 - Psyr_0407 0.70 +1.2
437,921 + Psyr_0407 0.70 -0.6
438,294 + Psyr_0407 0.88 +0.9
438,401 + +0.4
438,410 + +1.2
438,410 + +1.6
438,410 + +3.2
438,411 - +2.3
438,411 - +2.7
438,411 - -0.1
438,411 - +0.2
438,445 + +1.0
438,445 + -0.3
438,445 + +1.1
438,445 + +0.3
438,445 + +0.5
438,446 - +2.0
438,450 + +0.3
438,578 + -0.3

Or see this region's nucleotide sequence