Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0343 and Psyr_0344 are separated by 352 nucleotides Psyr_0344 and Psyr_0345 overlap by 4 nucleotides
Psyr_0343: Psyr_0343 - hypothetical protein, at 365,313 to 366,428
_0343
Psyr_0344: Psyr_0344 - Secretion protein HlyD, at 366,781 to 367,899
_0344
Psyr_0345: Psyr_0345 - Secretion protein HlyD, at 367,896 to 368,981
_0345
Position (kb) 366
367
368 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 365.781 kb on + strand, within Psyr_0343 at 365.781 kb on + strand, within Psyr_0343 at 365.781 kb on + strand, within Psyr_0343 at 365.781 kb on + strand, within Psyr_0343 at 365.860 kb on + strand, within Psyr_0343 at 365.860 kb on + strand, within Psyr_0343 at 365.860 kb on + strand, within Psyr_0343 at 365.860 kb on + strand, within Psyr_0343 at 365.860 kb on + strand, within Psyr_0343 at 365.860 kb on + strand, within Psyr_0343 at 365.861 kb on - strand, within Psyr_0343 at 365.861 kb on - strand, within Psyr_0343 at 365.861 kb on - strand, within Psyr_0343 at 365.861 kb on - strand, within Psyr_0343 at 365.864 kb on + strand, within Psyr_0343 at 365.864 kb on + strand, within Psyr_0343 at 365.864 kb on + strand, within Psyr_0343 at 365.865 kb on - strand, within Psyr_0343 at 365.865 kb on - strand, within Psyr_0343 at 365.865 kb on - strand, within Psyr_0343 at 365.865 kb on - strand, within Psyr_0343 at 365.865 kb on - strand, within Psyr_0343 at 365.922 kb on - strand, within Psyr_0343 at 365.922 kb on - strand, within Psyr_0343 at 365.988 kb on - strand, within Psyr_0343 at 366.005 kb on - strand, within Psyr_0343 at 366.026 kb on - strand, within Psyr_0343 at 366.047 kb on + strand, within Psyr_0343 at 366.197 kb on + strand at 366.197 kb on + strand, within Psyr_0343 at 366.197 kb on + strand, within Psyr_0343 at 366.197 kb on + strand, within Psyr_0343 at 366.198 kb on - strand, within Psyr_0343 at 366.198 kb on - strand, within Psyr_0343 at 366.198 kb on - strand, within Psyr_0343 at 366.198 kb on - strand, within Psyr_0343 at 366.198 kb on - strand, within Psyr_0343 at 366.215 kb on + strand, within Psyr_0343 at 366.255 kb on + strand, within Psyr_0343 at 366.255 kb on + strand, within Psyr_0343 at 366.255 kb on + strand, within Psyr_0343 at 366.255 kb on + strand, within Psyr_0343 at 366.255 kb on + strand, within Psyr_0343 at 366.256 kb on - strand, within Psyr_0343 at 366.291 kb on - strand, within Psyr_0343 at 366.295 kb on + strand, within Psyr_0343 at 366.295 kb on + strand, within Psyr_0343 at 366.296 kb on - strand, within Psyr_0343 at 366.322 kb on + strand at 366.323 kb on - strand at 366.323 kb on - strand at 366.323 kb on - strand at 366.325 kb on + strand at 366.325 kb on + strand at 366.325 kb on + strand at 366.326 kb on - strand at 366.339 kb on - strand at 366.339 kb on - strand at 366.341 kb on + strand at 366.341 kb on + strand at 366.458 kb on + strand at 366.458 kb on + strand at 366.458 kb on + strand at 366.498 kb on + strand at 366.498 kb on + strand at 366.498 kb on + strand at 366.499 kb on - strand at 366.499 kb on - strand at 366.499 kb on - strand at 366.502 kb on + strand at 366.580 kb on + strand at 366.580 kb on + strand at 366.580 kb on + strand at 366.580 kb on + strand at 366.580 kb on + strand at 366.580 kb on + strand at 366.580 kb on + strand at 366.580 kb on + strand at 366.580 kb on + strand at 366.580 kb on + strand at 366.581 kb on - strand at 366.581 kb on - strand at 366.581 kb on - strand at 366.581 kb on - strand at 366.581 kb on - strand at 366.581 kb on - strand at 366.581 kb on - strand at 366.581 kb on - strand at 366.581 kb on - strand at 366.581 kb on - strand at 366.581 kb on - strand at 366.584 kb on + strand at 366.584 kb on + strand at 366.584 kb on + strand at 366.584 kb on + strand at 366.584 kb on + strand at 366.584 kb on + strand at 366.584 kb on + strand at 366.584 kb on + strand at 366.584 kb on + strand at 366.584 kb on + strand at 366.584 kb on + strand at 366.584 kb on + strand at 366.585 kb on - strand at 366.585 kb on - strand at 366.585 kb on - strand at 366.585 kb on - strand at 366.585 kb on - strand at 366.585 kb on - strand at 366.585 kb on - strand at 366.585 kb on - strand at 366.585 kb on - strand at 366.585 kb on - strand at 366.585 kb on - strand at 366.585 kb on - strand at 366.585 kb on - strand at 366.585 kb on - strand at 366.585 kb on - strand at 366.585 kb on - strand at 366.585 kb on - strand at 366.585 kb on - strand at 366.585 kb on - strand at 366.645 kb on + strand at 366.645 kb on + strand at 366.645 kb on + strand at 366.645 kb on + strand at 366.646 kb on - strand at 366.646 kb on - strand at 366.646 kb on - strand at 366.646 kb on - strand at 366.648 kb on - strand at 366.648 kb on - strand at 366.648 kb on - strand at 366.679 kb on + strand at 366.679 kb on + strand at 366.680 kb on - strand at 366.721 kb on - strand at 366.792 kb on + strand at 366.793 kb on - strand at 366.793 kb on - strand at 366.807 kb on + strand at 366.807 kb on + strand at 366.807 kb on + strand at 366.807 kb on + strand at 367.177 kb on + strand, within Psyr_0344 at 367.177 kb on + strand, within Psyr_0344 at 367.178 kb on - strand, within Psyr_0344 at 367.179 kb on + strand, within Psyr_0344 at 367.180 kb on - strand, within Psyr_0344 at 367.180 kb on - strand, within Psyr_0344 at 367.180 kb on - strand, within Psyr_0344 at 367.180 kb on - strand, within Psyr_0344 at 367.195 kb on + strand, within Psyr_0344 at 367.195 kb on + strand, within Psyr_0344 at 367.195 kb on + strand, within Psyr_0344 at 367.195 kb on + strand, within Psyr_0344 at 367.196 kb on - strand, within Psyr_0344 at 367.417 kb on + strand, within Psyr_0344 at 367.418 kb on - strand, within Psyr_0344 at 367.418 kb on - strand, within Psyr_0344 at 367.515 kb on - strand, within Psyr_0344 at 367.542 kb on + strand, within Psyr_0344 at 367.700 kb on + strand, within Psyr_0344 at 367.832 kb on + strand at 367.832 kb on + strand at 367.832 kb on + strand at 367.833 kb on - strand at 367.833 kb on - strand at 367.948 kb on + strand at 367.949 kb on - strand at 367.949 kb on - strand at 368.128 kb on + strand, within Psyr_0345 at 368.129 kb on - strand, within Psyr_0345 at 368.534 kb on + strand, within Psyr_0345 at 368.566 kb on + strand, within Psyr_0345 at 368.566 kb on + strand, within Psyr_0345 at 368.567 kb on - strand, within Psyr_0345 at 368.654 kb on + strand, within Psyr_0345 at 368.654 kb on + strand, within Psyr_0345 at 368.654 kb on + strand, within Psyr_0345 at 368.655 kb on - strand, within Psyr_0345 at 368.655 kb on - strand, within Psyr_0345 at 368.655 kb on - strand, within Psyr_0345 at 368.723 kb on - strand, within Psyr_0345 at 368.850 kb on - strand, within Psyr_0345
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 365,781 + Psyr_0343 0.42 +1.6 365,781 + Psyr_0343 0.42 -2.5 365,781 + Psyr_0343 0.42 +0.4 365,781 + Psyr_0343 0.42 +0.1 365,860 + Psyr_0343 0.49 +0.5 365,860 + Psyr_0343 0.49 +0.0 365,860 + Psyr_0343 0.49 +0.3 365,860 + Psyr_0343 0.49 +2.5 365,860 + Psyr_0343 0.49 +0.4 365,860 + Psyr_0343 0.49 -0.9 365,861 - Psyr_0343 0.49 -1.3 365,861 - Psyr_0343 0.49 +0.6 365,861 - Psyr_0343 0.49 -0.2 365,861 - Psyr_0343 0.49 +0.5 365,864 + Psyr_0343 0.49 -0.2 365,864 + Psyr_0343 0.49 +0.2 365,864 + Psyr_0343 0.49 -0.2 365,865 - Psyr_0343 0.49 -0.2 365,865 - Psyr_0343 0.49 -1.6 365,865 - Psyr_0343 0.49 +1.1 365,865 - Psyr_0343 0.49 +0.6 365,865 - Psyr_0343 0.49 -1.2 365,922 - Psyr_0343 0.55 -0.3 365,922 - Psyr_0343 0.55 +0.5 365,988 - Psyr_0343 0.60 -0.2 366,005 - Psyr_0343 0.62 +0.1 366,026 - Psyr_0343 0.64 +0.5 366,047 + Psyr_0343 0.66 +1.0 366,197 + -2.3 366,197 + Psyr_0343 0.79 -0.3 366,197 + Psyr_0343 0.79 -1.5 366,197 + Psyr_0343 0.79 -0.1 366,198 - Psyr_0343 0.79 +0.9 366,198 - Psyr_0343 0.79 -0.4 366,198 - Psyr_0343 0.79 -0.5 366,198 - Psyr_0343 0.79 -0.8 366,198 - Psyr_0343 0.79 +1.0 366,215 + Psyr_0343 0.81 -0.4 366,255 + Psyr_0343 0.84 -1.2 366,255 + Psyr_0343 0.84 +0.2 366,255 + Psyr_0343 0.84 +0.9 366,255 + Psyr_0343 0.84 +0.7 366,255 + Psyr_0343 0.84 +0.8 366,256 - Psyr_0343 0.84 +0.1 366,291 - Psyr_0343 0.88 +1.7 366,295 + Psyr_0343 0.88 +0.2 366,295 + Psyr_0343 0.88 +1.0 366,296 - Psyr_0343 0.88 +2.4 366,322 + +0.6 366,323 - +0.7 366,323 - +0.1 366,323 - -0.9 366,325 + -0.2 366,325 + +1.0 366,325 + +2.1 366,326 - -0.8 366,339 - -2.8 366,339 - -1.1 366,341 + -0.0 366,341 + +0.1 366,458 + +1.2 366,458 + -0.3 366,458 + +0.4 366,498 + -1.0 366,498 + -2.4 366,498 + -1.4 366,499 - -3.1 366,499 - -0.1 366,499 - -1.2 366,502 + -0.2 366,580 + +0.4 366,580 + -0.6 366,580 + -0.4 366,580 + +0.7 366,580 + +0.7 366,580 + +0.8 366,580 + +2.1 366,580 + +0.6 366,580 + +0.6 366,580 + -0.1 366,581 - -1.2 366,581 - -1.1 366,581 - -2.4 366,581 - +0.6 366,581 - -1.8 366,581 - +0.2 366,581 - -1.1 366,581 - -0.9 366,581 - -0.5 366,581 - -0.2 366,581 - -0.2 366,584 + +0.3 366,584 + +0.2 366,584 + +1.7 366,584 + -0.8 366,584 + -0.3 366,584 + -0.6 366,584 + -0.6 366,584 + +0.4 366,584 + -0.4 366,584 + -2.3 366,584 + -1.0 366,584 + +0.7 366,585 - -1.0 366,585 - -1.5 366,585 - +0.2 366,585 - -1.6 366,585 - -0.1 366,585 - -1.1 366,585 - +0.0 366,585 - -0.0 366,585 - -0.9 366,585 - -1.8 366,585 - -1.5 366,585 - -2.5 366,585 - -1.2 366,585 - -0.4 366,585 - -0.4 366,585 - -0.2 366,585 - -1.3 366,585 - +0.6 366,585 - -3.7 366,645 + -0.5 366,645 + +1.4 366,645 + -0.4 366,645 + -0.9 366,646 - +0.9 366,646 - -1.0 366,646 - -0.6 366,646 - -2.2 366,648 - +0.5 366,648 - +4.1 366,648 - -1.6 366,679 + -0.7 366,679 + +1.4 366,680 - -0.1 366,721 - -0.1 366,792 + -0.1 366,793 - +0.8 366,793 - -2.4 366,807 + +0.0 366,807 + +0.1 366,807 + +0.3 366,807 + -1.2 367,177 + Psyr_0344 0.35 +0.2 367,177 + Psyr_0344 0.35 +0.8 367,178 - Psyr_0344 0.35 +0.3 367,179 + Psyr_0344 0.36 +0.8 367,180 - Psyr_0344 0.36 +0.2 367,180 - Psyr_0344 0.36 +0.4 367,180 - Psyr_0344 0.36 +0.1 367,180 - Psyr_0344 0.36 +1.1 367,195 + Psyr_0344 0.37 -0.9 367,195 + Psyr_0344 0.37 -0.7 367,195 + Psyr_0344 0.37 +0.5 367,195 + Psyr_0344 0.37 +0.4 367,196 - Psyr_0344 0.37 -0.9 367,417 + Psyr_0344 0.57 -1.9 367,418 - Psyr_0344 0.57 -1.8 367,418 - Psyr_0344 0.57 +0.1 367,515 - Psyr_0344 0.66 -0.7 367,542 + Psyr_0344 0.68 +1.4 367,700 + Psyr_0344 0.82 -1.0 367,832 + +0.7 367,832 + +0.4 367,832 + -3.7 367,833 - +0.2 367,833 - +1.4 367,948 + +0.3 367,949 - +0.3 367,949 - +0.4 368,128 + Psyr_0345 0.21 -0.5 368,129 - Psyr_0345 0.21 -1.3 368,534 + Psyr_0345 0.59 +0.5 368,566 + Psyr_0345 0.62 +1.2 368,566 + Psyr_0345 0.62 +0.3 368,567 - Psyr_0345 0.62 +1.2 368,654 + Psyr_0345 0.70 +0.1 368,654 + Psyr_0345 0.70 +1.7 368,654 + Psyr_0345 0.70 -0.3 368,655 - Psyr_0345 0.70 +0.8 368,655 - Psyr_0345 0.70 +0.2 368,655 - Psyr_0345 0.70 -0.7 368,723 - Psyr_0345 0.76 -1.8 368,850 - Psyr_0345 0.88 +0.2
Or see this region's nucleotide sequence