Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0317 and Psyr_0318 are separated by 303 nucleotides Psyr_0318 and Psyr_0319 overlap by 4 nucleotides Psyr_0319 and Psyr_0320 are separated by 150 nucleotides Psyr_0320 and Psyr_0321 are separated by 75 nucleotides
Psyr_0317: Psyr_0317 - 5-formyltetrahydrofolate cyclo-ligase, at 335,260 to 335,871
_0317
Psyr_0318: Psyr_0318 - cell division protein ZapA, at 336,175 to 336,504
_0318
Psyr_0319: Psyr_0319 - conserved hypothetical protein, at 336,501 to 336,710
_0319
Psyr_0320: Psyr_0320 - Protein of unknown function UPF0149, at 336,861 to 337,430
_0320
Psyr_0321: Psyr_0321 - aminopeptidase P, Metallo peptidase, MEROPS family M24B, at 337,506 to 338,840
_0321
Position (kb) 336
337
338 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 335.993 kb on + strand at 336.039 kb on - strand at 336.154 kb on + strand at 336.154 kb on + strand at 336.154 kb on + strand at 336.154 kb on + strand at 336.155 kb on - strand at 336.155 kb on - strand at 336.280 kb on + strand, within Psyr_0318 at 336.280 kb on + strand, within Psyr_0318 at 336.280 kb on + strand, within Psyr_0318 at 336.280 kb on + strand, within Psyr_0318 at 336.280 kb on + strand, within Psyr_0318 at 336.280 kb on + strand, within Psyr_0318 at 336.281 kb on - strand, within Psyr_0318 at 336.281 kb on - strand, within Psyr_0318 at 336.281 kb on - strand, within Psyr_0318 at 336.281 kb on - strand, within Psyr_0318 at 336.281 kb on - strand, within Psyr_0318 at 336.281 kb on - strand, within Psyr_0318 at 336.458 kb on + strand, within Psyr_0318 at 336.458 kb on + strand, within Psyr_0318 at 336.458 kb on + strand, within Psyr_0318 at 336.459 kb on - strand, within Psyr_0318 at 336.618 kb on + strand, within Psyr_0319 at 336.618 kb on + strand, within Psyr_0319 at 336.618 kb on + strand, within Psyr_0319 at 336.618 kb on + strand, within Psyr_0319 at 336.619 kb on - strand, within Psyr_0319 at 336.619 kb on - strand, within Psyr_0319 at 336.619 kb on - strand, within Psyr_0319 at 336.621 kb on + strand, within Psyr_0319 at 336.621 kb on + strand, within Psyr_0319 at 336.621 kb on + strand, within Psyr_0319 at 336.621 kb on + strand, within Psyr_0319 at 336.621 kb on + strand, within Psyr_0319 at 336.621 kb on + strand, within Psyr_0319 at 336.621 kb on + strand, within Psyr_0319 at 336.621 kb on + strand, within Psyr_0319 at 336.621 kb on + strand, within Psyr_0319 at 336.621 kb on + strand, within Psyr_0319 at 336.621 kb on + strand, within Psyr_0319 at 336.622 kb on - strand, within Psyr_0319 at 336.641 kb on + strand, within Psyr_0319 at 336.655 kb on + strand, within Psyr_0319 at 336.655 kb on + strand, within Psyr_0319 at 336.655 kb on + strand, within Psyr_0319 at 336.656 kb on - strand, within Psyr_0319 at 336.656 kb on - strand, within Psyr_0319 at 336.656 kb on - strand, within Psyr_0319 at 336.656 kb on - strand, within Psyr_0319 at 336.725 kb on + strand at 336.725 kb on + strand at 336.725 kb on + strand at 336.725 kb on + strand at 336.808 kb on + strand at 336.813 kb on + strand at 336.813 kb on + strand at 336.813 kb on + strand at 336.813 kb on + strand at 336.829 kb on + strand at 336.829 kb on + strand at 336.829 kb on + strand at 336.853 kb on - strand at 336.856 kb on + strand at 336.882 kb on + strand at 336.920 kb on + strand, within Psyr_0320 at 336.925 kb on + strand, within Psyr_0320 at 337.332 kb on + strand, within Psyr_0320 at 337.350 kb on + strand, within Psyr_0320 at 337.350 kb on + strand, within Psyr_0320 at 337.350 kb on + strand, within Psyr_0320 at 337.350 kb on + strand, within Psyr_0320 at 337.350 kb on + strand, within Psyr_0320 at 337.350 kb on + strand, within Psyr_0320 at 337.350 kb on + strand, within Psyr_0320 at 337.350 kb on + strand, within Psyr_0320 at 337.350 kb on + strand, within Psyr_0320 at 337.350 kb on + strand, within Psyr_0320 at 337.350 kb on + strand, within Psyr_0320 at 337.350 kb on + strand, within Psyr_0320 at 337.351 kb on - strand, within Psyr_0320 at 337.351 kb on - strand, within Psyr_0320 at 337.530 kb on + strand at 337.716 kb on + strand, within Psyr_0321 at 337.725 kb on + strand, within Psyr_0321 at 337.725 kb on + strand, within Psyr_0321 at 337.725 kb on + strand, within Psyr_0321 at 337.725 kb on + strand, within Psyr_0321 at 337.725 kb on + strand, within Psyr_0321 at 337.725 kb on + strand, within Psyr_0321 at 337.725 kb on + strand, within Psyr_0321 at 337.725 kb on + strand, within Psyr_0321 at 337.725 kb on + strand, within Psyr_0321 at 337.725 kb on + strand, within Psyr_0321 at 337.725 kb on + strand, within Psyr_0321 at 337.729 kb on + strand, within Psyr_0321 at 337.729 kb on + strand, within Psyr_0321 at 337.729 kb on + strand, within Psyr_0321 at 337.729 kb on + strand, within Psyr_0321 at 337.729 kb on + strand, within Psyr_0321 at 337.729 kb on + strand, within Psyr_0321 at 337.729 kb on + strand, within Psyr_0321 at 337.730 kb on - strand, within Psyr_0321 at 337.730 kb on - strand, within Psyr_0321 at 337.730 kb on - strand, within Psyr_0321 at 337.730 kb on - strand, within Psyr_0321 at 337.730 kb on - strand, within Psyr_0321 at 337.730 kb on - strand, within Psyr_0321 at 337.887 kb on + strand, within Psyr_0321 at 337.887 kb on + strand, within Psyr_0321 at 338.028 kb on + strand, within Psyr_0321 at 338.028 kb on + strand, within Psyr_0321 at 338.028 kb on + strand, within Psyr_0321 at 338.028 kb on + strand, within Psyr_0321 at 338.072 kb on + strand, within Psyr_0321 at 338.190 kb on + strand, within Psyr_0321 at 338.307 kb on + strand, within Psyr_0321 at 338.308 kb on - strand, within Psyr_0321 at 338.308 kb on - strand, within Psyr_0321 at 338.373 kb on + strand, within Psyr_0321 at 338.373 kb on + strand, within Psyr_0321 at 338.373 kb on + strand, within Psyr_0321 at 338.373 kb on + strand, within Psyr_0321 at 338.373 kb on + strand, within Psyr_0321 at 338.374 kb on - strand, within Psyr_0321 at 338.374 kb on - strand, within Psyr_0321
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 335,993 + +1.1 336,039 - +1.0 336,154 + +0.6 336,154 + +0.2 336,154 + +1.4 336,154 + +0.3 336,155 - +0.2 336,155 - +0.5 336,280 + Psyr_0318 0.32 +0.7 336,280 + Psyr_0318 0.32 +1.6 336,280 + Psyr_0318 0.32 -2.9 336,280 + Psyr_0318 0.32 +0.1 336,280 + Psyr_0318 0.32 +1.5 336,280 + Psyr_0318 0.32 +0.6 336,281 - Psyr_0318 0.32 -0.8 336,281 - Psyr_0318 0.32 +0.6 336,281 - Psyr_0318 0.32 +2.9 336,281 - Psyr_0318 0.32 +2.4 336,281 - Psyr_0318 0.32 -0.3 336,281 - Psyr_0318 0.32 +0.7 336,458 + Psyr_0318 0.86 -0.3 336,458 + Psyr_0318 0.86 +0.7 336,458 + Psyr_0318 0.86 -0.9 336,459 - Psyr_0318 0.86 +1.3 336,618 + Psyr_0319 0.56 +0.7 336,618 + Psyr_0319 0.56 +0.7 336,618 + Psyr_0319 0.56 -0.6 336,618 + Psyr_0319 0.56 +1.3 336,619 - Psyr_0319 0.56 +1.2 336,619 - Psyr_0319 0.56 +0.2 336,619 - Psyr_0319 0.56 -0.0 336,621 + Psyr_0319 0.57 +1.7 336,621 + Psyr_0319 0.57 +0.5 336,621 + Psyr_0319 0.57 +1.1 336,621 + Psyr_0319 0.57 -1.8 336,621 + Psyr_0319 0.57 -0.6 336,621 + Psyr_0319 0.57 +0.5 336,621 + Psyr_0319 0.57 +0.5 336,621 + Psyr_0319 0.57 +1.6 336,621 + Psyr_0319 0.57 -0.9 336,621 + Psyr_0319 0.57 -2.4 336,621 + Psyr_0319 0.57 +0.6 336,622 - Psyr_0319 0.58 -0.7 336,641 + Psyr_0319 0.67 -0.5 336,655 + Psyr_0319 0.73 +0.6 336,655 + Psyr_0319 0.73 +1.5 336,655 + Psyr_0319 0.73 +0.4 336,656 - Psyr_0319 0.74 -0.0 336,656 - Psyr_0319 0.74 -1.0 336,656 - Psyr_0319 0.74 -2.2 336,656 - Psyr_0319 0.74 -3.0 336,725 + +0.6 336,725 + +0.2 336,725 + +0.8 336,725 + +1.2 336,808 + +1.3 336,813 + +0.1 336,813 + +0.1 336,813 + +1.3 336,813 + +0.3 336,829 + +1.1 336,829 + -1.3 336,829 + +1.4 336,853 - -0.4 336,856 + +0.1 336,882 + -1.5 336,920 + Psyr_0320 0.10 +1.1 336,925 + Psyr_0320 0.11 +0.5 337,332 + Psyr_0320 0.83 +1.3 337,350 + Psyr_0320 0.86 +1.6 337,350 + Psyr_0320 0.86 +0.5 337,350 + Psyr_0320 0.86 +0.1 337,350 + Psyr_0320 0.86 +1.2 337,350 + Psyr_0320 0.86 +3.3 337,350 + Psyr_0320 0.86 +0.5 337,350 + Psyr_0320 0.86 +0.6 337,350 + Psyr_0320 0.86 +1.2 337,350 + Psyr_0320 0.86 +0.4 337,350 + Psyr_0320 0.86 +1.2 337,350 + Psyr_0320 0.86 -1.2 337,350 + Psyr_0320 0.86 -0.2 337,351 - Psyr_0320 0.86 -1.9 337,351 - Psyr_0320 0.86 +0.4 337,530 + +0.5 337,716 + Psyr_0321 0.16 +0.5 337,725 + Psyr_0321 0.16 -2.1 337,725 + Psyr_0321 0.16 -0.2 337,725 + Psyr_0321 0.16 -0.6 337,725 + Psyr_0321 0.16 +0.1 337,725 + Psyr_0321 0.16 -1.8 337,725 + Psyr_0321 0.16 +0.2 337,725 + Psyr_0321 0.16 +0.1 337,725 + Psyr_0321 0.16 +0.1 337,725 + Psyr_0321 0.16 +0.8 337,725 + Psyr_0321 0.16 +2.7 337,725 + Psyr_0321 0.16 -0.9 337,729 + Psyr_0321 0.17 +0.3 337,729 + Psyr_0321 0.17 +1.3 337,729 + Psyr_0321 0.17 +1.5 337,729 + Psyr_0321 0.17 -1.6 337,729 + Psyr_0321 0.17 -0.9 337,729 + Psyr_0321 0.17 -0.0 337,729 + Psyr_0321 0.17 -0.9 337,730 - Psyr_0321 0.17 -0.7 337,730 - Psyr_0321 0.17 -2.2 337,730 - Psyr_0321 0.17 -0.9 337,730 - Psyr_0321 0.17 -3.4 337,730 - Psyr_0321 0.17 -1.6 337,730 - Psyr_0321 0.17 -0.3 337,887 + Psyr_0321 0.29 -0.2 337,887 + Psyr_0321 0.29 +1.5 338,028 + Psyr_0321 0.39 +1.5 338,028 + Psyr_0321 0.39 -2.6 338,028 + Psyr_0321 0.39 +0.2 338,028 + Psyr_0321 0.39 -3.1 338,072 + Psyr_0321 0.42 -0.6 338,190 + Psyr_0321 0.51 +0.9 338,307 + Psyr_0321 0.60 +0.3 338,308 - Psyr_0321 0.60 -1.6 338,308 - Psyr_0321 0.60 +0.2 338,373 + Psyr_0321 0.65 -1.9 338,373 + Psyr_0321 0.65 -0.7 338,373 + Psyr_0321 0.65 +0.2 338,373 + Psyr_0321 0.65 +0.3 338,373 + Psyr_0321 0.65 -1.0 338,374 - Psyr_0321 0.65 -2.9 338,374 - Psyr_0321 0.65 -0.4
Or see this region's nucleotide sequence