Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0313 and Psyr_0314 are separated by 130 nucleotides Psyr_0314 and Psyr_0315 are separated by 6 nucleotides Psyr_0315 and Psyr_0316 are separated by 193 nucleotides Psyr_0316 and Psyr_0317 are separated by 45 nucleotides
Psyr_0313: Psyr_0313 - Zinc-containing alcohol dehydrogenase superfamily, at 332,888 to 333,874
_0313
Psyr_0314: Psyr_0314 - Flagellar basal body-associated protein FliL, at 334,005 to 334,412
_0314
Psyr_0315: Psyr_0315 - Protein of unknown function DUF589, at 334,419 to 334,868
_0315
Psyr_0316: Psyr_0316 - conserved hypothetical protein, at 335,062 to 335,214
_0316
Psyr_0317: Psyr_0317 - 5-formyltetrahydrofolate cyclo-ligase, at 335,260 to 335,871
_0317
Position (kb) 334
335 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 333.440 kb on + strand, within Psyr_0313 at 333.567 kb on + strand, within Psyr_0313 at 333.567 kb on + strand, within Psyr_0313 at 333.568 kb on - strand, within Psyr_0313 at 333.568 kb on - strand, within Psyr_0313 at 333.568 kb on - strand, within Psyr_0313 at 333.723 kb on + strand, within Psyr_0313 at 333.723 kb on + strand, within Psyr_0313 at 333.724 kb on - strand, within Psyr_0313 at 333.724 kb on - strand, within Psyr_0313 at 333.724 kb on - strand, within Psyr_0313 at 333.724 kb on - strand, within Psyr_0313 at 333.861 kb on - strand at 333.861 kb on - strand at 333.914 kb on + strand at 333.914 kb on + strand at 333.915 kb on - strand at 333.915 kb on - strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.970 kb on + strand at 333.970 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.976 kb on - strand at 333.976 kb on - strand at 333.976 kb on - strand at 333.976 kb on - strand at 333.976 kb on - strand at 333.989 kb on + strand at 333.989 kb on + strand at 333.990 kb on - strand at 333.992 kb on + strand at 334.098 kb on + strand, within Psyr_0314 at 334.098 kb on + strand, within Psyr_0314 at 334.098 kb on + strand, within Psyr_0314 at 334.098 kb on + strand, within Psyr_0314 at 334.098 kb on + strand, within Psyr_0314 at 334.099 kb on - strand, within Psyr_0314 at 334.099 kb on - strand, within Psyr_0314 at 334.099 kb on - strand, within Psyr_0314 at 334.099 kb on - strand, within Psyr_0314 at 334.131 kb on + strand, within Psyr_0314 at 334.131 kb on + strand, within Psyr_0314 at 334.131 kb on + strand, within Psyr_0314 at 334.131 kb on + strand, within Psyr_0314 at 334.132 kb on - strand, within Psyr_0314 at 334.132 kb on - strand, within Psyr_0314 at 334.132 kb on - strand, within Psyr_0314 at 334.132 kb on - strand, within Psyr_0314 at 334.132 kb on - strand, within Psyr_0314 at 334.164 kb on + strand, within Psyr_0314 at 334.164 kb on + strand, within Psyr_0314 at 334.164 kb on + strand, within Psyr_0314 at 334.164 kb on + strand, within Psyr_0314 at 334.164 kb on + strand, within Psyr_0314 at 334.165 kb on - strand, within Psyr_0314 at 334.165 kb on - strand, within Psyr_0314 at 334.165 kb on - strand, within Psyr_0314 at 334.165 kb on - strand, within Psyr_0314 at 334.394 kb on + strand at 334.395 kb on - strand at 334.531 kb on + strand, within Psyr_0315 at 334.661 kb on - strand, within Psyr_0315 at 334.720 kb on + strand, within Psyr_0315 at 334.774 kb on + strand, within Psyr_0315 at 334.774 kb on + strand, within Psyr_0315 at 334.774 kb on + strand, within Psyr_0315 at 334.774 kb on + strand, within Psyr_0315 at 334.775 kb on - strand, within Psyr_0315 at 334.896 kb on - strand at 334.945 kb on + strand at 334.945 kb on + strand at 334.945 kb on + strand at 334.945 kb on + strand at 334.946 kb on - strand at 335.011 kb on + strand at 335.035 kb on + strand at 335.035 kb on + strand at 335.035 kb on + strand at 335.081 kb on + strand, within Psyr_0316 at 335.226 kb on - strand at 335.226 kb on - strand at 335.279 kb on + strand at 335.279 kb on + strand at 335.279 kb on + strand at 335.279 kb on + strand at 335.279 kb on + strand at 335.279 kb on + strand at 335.279 kb on + strand at 335.279 kb on + strand at 335.280 kb on - strand at 335.280 kb on - strand at 335.280 kb on - strand at 335.280 kb on - strand at 335.280 kb on - strand at 335.280 kb on - strand at 335.280 kb on - strand at 335.280 kb on - strand at 335.280 kb on - strand at 335.314 kb on + strand at 335.314 kb on + strand at 335.315 kb on - strand at 335.315 kb on - strand at 335.315 kb on - strand at 335.374 kb on + strand, within Psyr_0317 at 335.411 kb on + strand, within Psyr_0317 at 335.430 kb on - strand, within Psyr_0317 at 335.430 kb on - strand, within Psyr_0317 at 335.476 kb on + strand, within Psyr_0317 at 335.476 kb on + strand, within Psyr_0317 at 335.639 kb on + strand, within Psyr_0317 at 335.639 kb on + strand, within Psyr_0317 at 335.639 kb on + strand, within Psyr_0317 at 335.639 kb on + strand, within Psyr_0317 at 335.639 kb on + strand, within Psyr_0317 at 335.639 kb on + strand, within Psyr_0317 at 335.639 kb on + strand, within Psyr_0317 at 335.640 kb on - strand, within Psyr_0317 at 335.640 kb on - strand, within Psyr_0317 at 335.640 kb on - strand, within Psyr_0317 at 335.640 kb on - strand, within Psyr_0317 at 335.640 kb on - strand, within Psyr_0317 at 335.765 kb on + strand, within Psyr_0317 at 335.766 kb on - strand, within Psyr_0317 at 335.766 kb on - strand, within Psyr_0317 at 335.770 kb on - strand, within Psyr_0317 at 335.824 kb on + strand at 335.825 kb on - strand at 335.825 kb on - strand at 335.825 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 333,440 + Psyr_0313 0.56 -0.5 333,567 + Psyr_0313 0.69 +0.5 333,567 + Psyr_0313 0.69 +3.2 333,568 - Psyr_0313 0.69 -0.8 333,568 - Psyr_0313 0.69 -0.2 333,568 - Psyr_0313 0.69 -0.0 333,723 + Psyr_0313 0.85 -1.1 333,723 + Psyr_0313 0.85 -1.2 333,724 - Psyr_0313 0.85 -1.4 333,724 - Psyr_0313 0.85 +0.1 333,724 - Psyr_0313 0.85 +0.9 333,724 - Psyr_0313 0.85 -1.3 333,861 - -0.4 333,861 - -0.3 333,914 + +0.9 333,914 + -0.1 333,915 - +0.6 333,915 - +0.5 333,963 + +0.4 333,963 + -0.2 333,963 + -1.2 333,963 + +1.1 333,963 + +0.5 333,963 + -0.2 333,963 + -0.3 333,963 + +0.4 333,963 + -0.8 333,963 + +0.1 333,963 + +0.4 333,963 + -0.1 333,963 + -1.1 333,963 + +0.0 333,964 - +2.9 333,964 - -0.2 333,964 - +0.8 333,964 - +0.2 333,964 - +0.0 333,964 - -0.2 333,964 - -0.3 333,964 - +0.3 333,964 - -0.0 333,964 - -0.9 333,964 - -1.5 333,964 - -2.3 333,964 - -0.6 333,964 - -0.1 333,964 - -0.7 333,964 - +1.9 333,964 - -3.5 333,964 - +0.2 333,964 - -3.2 333,964 - -0.6 333,964 - -0.5 333,964 - +1.1 333,964 - +0.0 333,964 - -0.8 333,964 - +0.4 333,970 + -0.4 333,970 + +0.1 333,975 + -1.1 333,975 + +0.3 333,975 + +1.1 333,975 + +0.1 333,975 + -1.4 333,975 + +0.1 333,975 + -0.0 333,975 + +0.5 333,975 + +0.6 333,975 + +1.0 333,975 + +1.3 333,975 + -0.4 333,976 - -1.9 333,976 - -1.1 333,976 - -0.6 333,976 - -1.8 333,976 - -0.7 333,989 + -0.6 333,989 + +0.2 333,990 - -2.2 333,992 + +1.3 334,098 + Psyr_0314 0.23 +1.1 334,098 + Psyr_0314 0.23 -1.6 334,098 + Psyr_0314 0.23 +0.5 334,098 + Psyr_0314 0.23 +0.4 334,098 + Psyr_0314 0.23 -0.8 334,099 - Psyr_0314 0.23 +0.9 334,099 - Psyr_0314 0.23 +0.7 334,099 - Psyr_0314 0.23 +0.8 334,099 - Psyr_0314 0.23 +0.4 334,131 + Psyr_0314 0.31 -1.0 334,131 + Psyr_0314 0.31 -0.4 334,131 + Psyr_0314 0.31 +1.3 334,131 + Psyr_0314 0.31 +1.4 334,132 - Psyr_0314 0.31 +1.0 334,132 - Psyr_0314 0.31 -1.0 334,132 - Psyr_0314 0.31 -1.4 334,132 - Psyr_0314 0.31 -0.5 334,132 - Psyr_0314 0.31 +0.7 334,164 + Psyr_0314 0.39 +1.7 334,164 + Psyr_0314 0.39 -0.7 334,164 + Psyr_0314 0.39 -0.2 334,164 + Psyr_0314 0.39 +0.4 334,164 + Psyr_0314 0.39 +0.3 334,165 - Psyr_0314 0.39 +0.2 334,165 - Psyr_0314 0.39 -0.0 334,165 - Psyr_0314 0.39 +0.3 334,165 - Psyr_0314 0.39 +0.2 334,394 + -0.2 334,395 - -1.2 334,531 + Psyr_0315 0.25 -0.4 334,661 - Psyr_0315 0.54 +0.0 334,720 + Psyr_0315 0.67 +0.5 334,774 + Psyr_0315 0.79 -1.6 334,774 + Psyr_0315 0.79 -0.1 334,774 + Psyr_0315 0.79 -0.3 334,774 + Psyr_0315 0.79 +0.5 334,775 - Psyr_0315 0.79 +0.7 334,896 - -0.1 334,945 + +0.4 334,945 + -0.4 334,945 + -0.3 334,945 + +0.2 334,946 - +3.0 335,011 + -0.3 335,035 + +1.6 335,035 + -0.4 335,035 + -1.6 335,081 + Psyr_0316 0.12 -0.2 335,226 - +0.6 335,226 - -0.1 335,279 + +1.3 335,279 + +0.9 335,279 + -2.8 335,279 + +0.4 335,279 + -0.6 335,279 + +0.4 335,279 + +1.5 335,279 + -0.6 335,280 - +0.3 335,280 - -0.9 335,280 - +0.8 335,280 - -0.9 335,280 - -1.1 335,280 - +0.1 335,280 - -0.1 335,280 - -0.7 335,280 - -1.0 335,314 + +0.2 335,314 + -0.8 335,315 - +0.7 335,315 - +0.3 335,315 - -0.4 335,374 + Psyr_0317 0.19 -1.1 335,411 + Psyr_0317 0.25 +0.0 335,430 - Psyr_0317 0.28 -0.3 335,430 - Psyr_0317 0.28 -1.2 335,476 + Psyr_0317 0.35 +1.1 335,476 + Psyr_0317 0.35 +1.0 335,639 + Psyr_0317 0.62 -0.7 335,639 + Psyr_0317 0.62 -0.3 335,639 + Psyr_0317 0.62 +0.4 335,639 + Psyr_0317 0.62 -0.6 335,639 + Psyr_0317 0.62 -0.4 335,639 + Psyr_0317 0.62 -2.6 335,639 + Psyr_0317 0.62 -2.3 335,640 - Psyr_0317 0.62 -1.2 335,640 - Psyr_0317 0.62 +1.5 335,640 - Psyr_0317 0.62 -1.8 335,640 - Psyr_0317 0.62 -0.6 335,640 - Psyr_0317 0.62 -0.8 335,765 + Psyr_0317 0.83 -0.6 335,766 - Psyr_0317 0.83 -0.4 335,766 - Psyr_0317 0.83 -0.1 335,770 - Psyr_0317 0.83 -0.4 335,824 + -0.2 335,825 - +0.8 335,825 - +0.1 335,825 - -0.9
Or see this region's nucleotide sequence