Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0310 and Psyr_0311 are separated by 98 nucleotides Psyr_0311 and Psyr_0312 are separated by 119 nucleotides Psyr_0312 and Psyr_0313 are separated by 255 nucleotides
Psyr_0310: Psyr_0310 - HNH endonuclease:S-type Pyocin, at 329,734 to 331,704
_0310
Psyr_0311: Psyr_0311 - CDS, at 331,803 to 332,150
_0311
Psyr_0312: Psyr_0312 - hypothetical protein, at 332,270 to 332,632
_0312
Psyr_0313: Psyr_0313 - Zinc-containing alcohol dehydrogenase superfamily, at 332,888 to 333,874
_0313
Position (kb)
332
333 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 331.399 kb on + strand, within Psyr_0310 at 331.399 kb on + strand, within Psyr_0310 at 331.400 kb on - strand, within Psyr_0310 at 331.474 kb on + strand, within Psyr_0310 at 331.474 kb on + strand, within Psyr_0310 at 331.475 kb on - strand, within Psyr_0310 at 331.477 kb on + strand, within Psyr_0310 at 331.477 kb on + strand, within Psyr_0310 at 331.477 kb on + strand, within Psyr_0310 at 331.478 kb on - strand, within Psyr_0310 at 331.478 kb on - strand, within Psyr_0310 at 331.478 kb on - strand, within Psyr_0310 at 331.478 kb on - strand, within Psyr_0310 at 331.494 kb on + strand, within Psyr_0310 at 331.498 kb on - strand, within Psyr_0310 at 331.498 kb on - strand, within Psyr_0310 at 331.504 kb on - strand, within Psyr_0310 at 331.504 kb on - strand, within Psyr_0310 at 331.504 kb on - strand, within Psyr_0310 at 331.532 kb on - strand at 331.590 kb on + strand at 331.591 kb on - strand at 331.608 kb on + strand at 331.608 kb on + strand at 331.609 kb on - strand at 331.611 kb on + strand at 331.648 kb on + strand at 331.673 kb on - strand at 331.676 kb on + strand at 331.676 kb on + strand at 331.677 kb on - strand at 331.678 kb on + strand at 331.678 kb on + strand at 331.678 kb on + strand at 331.678 kb on + strand at 331.679 kb on - strand at 331.679 kb on - strand at 331.679 kb on - strand at 331.679 kb on - strand at 331.679 kb on - strand at 331.679 kb on - strand at 331.679 kb on - strand at 331.679 kb on - strand at 331.679 kb on - strand at 331.680 kb on + strand at 331.680 kb on + strand at 331.681 kb on - strand at 331.705 kb on - strand at 331.716 kb on + strand at 331.766 kb on - strand at 331.792 kb on + strand at 331.792 kb on + strand at 331.793 kb on - strand at 331.793 kb on - strand at 331.793 kb on - strand at 331.804 kb on + strand at 331.804 kb on + strand at 332.230 kb on + strand at 332.230 kb on + strand at 332.233 kb on + strand at 332.264 kb on + strand at 332.338 kb on - strand, within Psyr_0312 at 332.356 kb on + strand, within Psyr_0312 at 332.356 kb on + strand, within Psyr_0312 at 332.356 kb on + strand, within Psyr_0312 at 332.359 kb on + strand, within Psyr_0312 at 332.360 kb on - strand, within Psyr_0312 at 332.362 kb on - strand, within Psyr_0312 at 332.452 kb on - strand, within Psyr_0312 at 332.452 kb on - strand, within Psyr_0312 at 332.452 kb on - strand, within Psyr_0312 at 332.488 kb on + strand, within Psyr_0312 at 332.495 kb on + strand, within Psyr_0312 at 332.495 kb on + strand, within Psyr_0312 at 332.550 kb on + strand, within Psyr_0312 at 332.550 kb on + strand, within Psyr_0312 at 332.551 kb on - strand, within Psyr_0312 at 332.551 kb on - strand, within Psyr_0312 at 332.565 kb on - strand, within Psyr_0312 at 332.565 kb on - strand, within Psyr_0312 at 332.637 kb on + strand at 332.645 kb on - strand at 332.647 kb on + strand at 332.647 kb on + strand at 332.647 kb on + strand at 332.648 kb on - strand at 332.648 kb on - strand at 332.648 kb on - strand at 332.709 kb on + strand at 332.709 kb on + strand at 332.712 kb on + strand at 332.712 kb on + strand at 332.716 kb on + strand at 332.716 kb on + strand at 332.716 kb on + strand at 332.716 kb on + strand at 332.717 kb on - strand at 332.749 kb on + strand at 332.749 kb on + strand at 332.772 kb on + strand at 332.772 kb on + strand at 332.773 kb on - strand at 332.832 kb on + strand at 332.878 kb on + strand at 332.879 kb on - strand at 332.882 kb on + strand at 333.215 kb on + strand, within Psyr_0313 at 333.216 kb on - strand, within Psyr_0313 at 333.216 kb on - strand, within Psyr_0313 at 333.216 kb on - strand, within Psyr_0313 at 333.237 kb on + strand, within Psyr_0313 at 333.238 kb on - strand, within Psyr_0313 at 333.238 kb on - strand, within Psyr_0313 at 333.238 kb on - strand, within Psyr_0313 at 333.238 kb on - strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.304 kb on - strand, within Psyr_0313 at 333.304 kb on - strand, within Psyr_0313 at 333.304 kb on - strand, within Psyr_0313 at 333.304 kb on - strand, within Psyr_0313 at 333.440 kb on + strand, within Psyr_0313 at 333.567 kb on + strand, within Psyr_0313 at 333.567 kb on + strand, within Psyr_0313 at 333.568 kb on - strand, within Psyr_0313 at 333.568 kb on - strand, within Psyr_0313 at 333.568 kb on - strand, within Psyr_0313
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 331,399 + Psyr_0310 0.84 +0.4 331,399 + Psyr_0310 0.84 -0.6 331,400 - Psyr_0310 0.85 +1.1 331,474 + Psyr_0310 0.88 -1.7 331,474 + Psyr_0310 0.88 -0.6 331,475 - Psyr_0310 0.88 -0.2 331,477 + Psyr_0310 0.88 -0.0 331,477 + Psyr_0310 0.88 -0.8 331,477 + Psyr_0310 0.88 +0.5 331,478 - Psyr_0310 0.88 -0.6 331,478 - Psyr_0310 0.88 -0.3 331,478 - Psyr_0310 0.88 -0.8 331,478 - Psyr_0310 0.88 +0.2 331,494 + Psyr_0310 0.89 +0.9 331,498 - Psyr_0310 0.89 +0.1 331,498 - Psyr_0310 0.89 -0.6 331,504 - Psyr_0310 0.90 -0.2 331,504 - Psyr_0310 0.90 -1.4 331,504 - Psyr_0310 0.90 -0.5 331,532 - -0.3 331,590 + +0.1 331,591 - -1.4 331,608 + +0.1 331,608 + -0.0 331,609 - -2.1 331,611 + -0.7 331,648 + +0.4 331,673 - +0.7 331,676 + +0.4 331,676 + -2.5 331,677 - +0.8 331,678 + -0.9 331,678 + +0.1 331,678 + +0.3 331,678 + +0.5 331,679 - -1.2 331,679 - -0.1 331,679 - +0.1 331,679 - -0.4 331,679 - -0.9 331,679 - +0.1 331,679 - +0.1 331,679 - -0.1 331,679 - -0.4 331,680 + -0.9 331,680 + -0.6 331,681 - -0.4 331,705 - -1.0 331,716 + -0.5 331,766 - -0.6 331,792 + -1.2 331,792 + -0.6 331,793 - +0.4 331,793 - -0.2 331,793 - +1.2 331,804 + -1.1 331,804 + -1.5 332,230 + -2.0 332,230 + -3.7 332,233 + -3.8 332,264 + -1.0 332,338 - Psyr_0312 0.19 -1.0 332,356 + Psyr_0312 0.24 +1.3 332,356 + Psyr_0312 0.24 -2.0 332,356 + Psyr_0312 0.24 -2.2 332,359 + Psyr_0312 0.25 -4.4 332,360 - Psyr_0312 0.25 -1.4 332,362 - Psyr_0312 0.25 -1.1 332,452 - Psyr_0312 0.50 -0.5 332,452 - Psyr_0312 0.50 -0.4 332,452 - Psyr_0312 0.50 +0.9 332,488 + Psyr_0312 0.60 +0.3 332,495 + Psyr_0312 0.62 -0.3 332,495 + Psyr_0312 0.62 -1.2 332,550 + Psyr_0312 0.77 -0.4 332,550 + Psyr_0312 0.77 -0.2 332,551 - Psyr_0312 0.77 +1.0 332,551 - Psyr_0312 0.77 -0.1 332,565 - Psyr_0312 0.81 -0.5 332,565 - Psyr_0312 0.81 -0.7 332,637 + -1.8 332,645 - -2.0 332,647 + -1.8 332,647 + -0.9 332,647 + -1.1 332,648 - +1.0 332,648 - -0.3 332,648 - -0.9 332,709 + +0.1 332,709 + +0.1 332,712 + -2.0 332,712 + -0.6 332,716 + -2.0 332,716 + -0.5 332,716 + +0.6 332,716 + -0.2 332,717 - -0.4 332,749 + -2.9 332,749 + -1.6 332,772 + +0.8 332,772 + -3.2 332,773 - -0.0 332,832 + -1.3 332,878 + +0.5 332,879 - +0.4 332,882 + +0.6 333,215 + Psyr_0313 0.33 +1.9 333,216 - Psyr_0313 0.33 -0.7 333,216 - Psyr_0313 0.33 +0.1 333,216 - Psyr_0313 0.33 -2.5 333,237 + Psyr_0313 0.35 +1.4 333,238 - Psyr_0313 0.35 -0.3 333,238 - Psyr_0313 0.35 +0.4 333,238 - Psyr_0313 0.35 -1.1 333,238 - Psyr_0313 0.35 +0.4 333,303 + Psyr_0313 0.42 -0.4 333,303 + Psyr_0313 0.42 +0.4 333,303 + Psyr_0313 0.42 -1.0 333,303 + Psyr_0313 0.42 +0.3 333,303 + Psyr_0313 0.42 -1.8 333,304 - Psyr_0313 0.42 -1.0 333,304 - Psyr_0313 0.42 -0.8 333,304 - Psyr_0313 0.42 -2.0 333,304 - Psyr_0313 0.42 +0.1 333,440 + Psyr_0313 0.56 -0.5 333,567 + Psyr_0313 0.69 +0.5 333,567 + Psyr_0313 0.69 +3.2 333,568 - Psyr_0313 0.69 -0.8 333,568 - Psyr_0313 0.69 -0.2 333,568 - Psyr_0313 0.69 -0.0
Or see this region's nucleotide sequence