Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0293

Experiment: KB with Novobiocin 250 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0292 and Psyr_0293 are separated by 16 nucleotidesPsyr_0293 and Psyr_0294 overlap by 14 nucleotides Psyr_0292: Psyr_0292 - porphobilinogen synthase, at 310,413 to 311,423 _0292 Psyr_0293: Psyr_0293 - Polyphosphate kinase, at 311,440 to 313,650 _0293 Psyr_0294: Psyr_0294 - Exopolyphosphatase, at 313,637 to 315,217 _0294 Position (kb) 311 312 313 314Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 311.445 kb on + strandat 311.522 kb on + strandat 311.523 kb on - strandat 311.523 kb on - strandat 311.596 kb on + strandat 311.596 kb on + strandat 311.596 kb on + strandat 311.596 kb on + strandat 311.596 kb on + strandat 311.596 kb on + strandat 311.596 kb on + strandat 311.596 kb on + strandat 311.597 kb on - strandat 311.597 kb on - strandat 311.597 kb on - strandat 311.597 kb on - strandat 311.597 kb on - strandat 311.597 kb on - strandat 311.597 kb on - strandat 311.597 kb on - strandat 311.597 kb on - strandat 311.597 kb on - strandat 311.597 kb on - strandat 311.597 kb on - strandat 311.597 kb on - strandat 311.963 kb on - strand, within Psyr_0293at 312.251 kb on - strand, within Psyr_0293at 312.362 kb on + strand, within Psyr_0293at 312.363 kb on - strand, within Psyr_0293at 312.445 kb on + strand, within Psyr_0293at 312.445 kb on + strand, within Psyr_0293at 312.446 kb on - strand, within Psyr_0293at 312.697 kb on + strand, within Psyr_0293at 312.856 kb on + strand, within Psyr_0293at 312.856 kb on + strand, within Psyr_0293at 312.926 kb on + strand, within Psyr_0293at 312.926 kb on + strand, within Psyr_0293at 312.926 kb on + strand, within Psyr_0293at 312.927 kb on - strand, within Psyr_0293at 312.927 kb on - strand, within Psyr_0293at 312.927 kb on - strand, within Psyr_0293at 312.927 kb on - strand, within Psyr_0293at 312.927 kb on - strand, within Psyr_0293at 312.927 kb on - strand, within Psyr_0293at 312.927 kb on - strand, within Psyr_0293at 312.927 kb on - strand, within Psyr_0293at 312.927 kb on - strand, within Psyr_0293at 312.955 kb on + strand, within Psyr_0293at 312.955 kb on + strand, within Psyr_0293at 312.956 kb on - strand, within Psyr_0293at 312.956 kb on - strand, within Psyr_0293at 312.956 kb on - strand, within Psyr_0293at 312.979 kb on + strand, within Psyr_0293at 312.979 kb on + strand, within Psyr_0293at 312.979 kb on + strand, within Psyr_0293at 312.979 kb on + strand, within Psyr_0293at 312.979 kb on + strand, within Psyr_0293at 312.980 kb on - strand, within Psyr_0293at 312.980 kb on - strand, within Psyr_0293at 312.980 kb on - strand, within Psyr_0293at 312.980 kb on - strand, within Psyr_0293at 312.980 kb on - strand, within Psyr_0293at 312.989 kb on - strand, within Psyr_0293at 313.225 kb on + strand, within Psyr_0293at 313.225 kb on + strand, within Psyr_0293at 313.225 kb on + strand, within Psyr_0293at 313.225 kb on + strand, within Psyr_0293at 313.225 kb on + strand, within Psyr_0293at 313.226 kb on - strand, within Psyr_0293at 313.226 kb on - strand, within Psyr_0293at 313.226 kb on - strand, within Psyr_0293at 313.226 kb on - strand, within Psyr_0293at 313.366 kb on + strand, within Psyr_0293at 313.366 kb on + strand, within Psyr_0293at 313.366 kb on + strand, within Psyr_0293at 313.366 kb on + strand, within Psyr_0293at 313.366 kb on + strand, within Psyr_0293at 313.366 kb on + strand, within Psyr_0293at 313.366 kb on + strand, within Psyr_0293at 313.366 kb on + strand, within Psyr_0293at 313.367 kb on - strand, within Psyr_0293at 313.367 kb on - strand, within Psyr_0293at 313.367 kb on - strand, within Psyr_0293at 313.367 kb on - strand, within Psyr_0293at 313.507 kb on + strandat 313.508 kb on - strandat 313.508 kb on - strandat 313.643 kb on + strandat 313.643 kb on + strandat 313.900 kb on + strand, within Psyr_0294at 313.984 kb on - strand, within Psyr_0294at 314.008 kb on - strand, within Psyr_0294at 314.067 kb on + strand, within Psyr_0294at 314.164 kb on - strand, within Psyr_0294at 314.198 kb on + strand, within Psyr_0294at 314.198 kb on + strand, within Psyr_0294at 314.198 kb on + strand, within Psyr_0294at 314.198 kb on + strand, within Psyr_0294at 314.199 kb on - strand, within Psyr_0294at 314.199 kb on - strand, within Psyr_0294at 314.199 kb on - strand, within Psyr_0294at 314.199 kb on - strand, within Psyr_0294at 314.233 kb on - strand, within Psyr_0294at 314.568 kb on + strand, within Psyr_0294at 314.569 kb on - strand, within Psyr_0294at 314.569 kb on - strand, within Psyr_0294at 314.580 kb on + strand, within Psyr_0294at 314.614 kb on - strand, within Psyr_0294

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml
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311,445 + +0.7
311,522 + +0.4
311,523 - +1.3
311,523 - +1.1
311,596 + +1.0
311,596 + +1.4
311,596 + +3.2
311,596 + +1.3
311,596 + +1.9
311,596 + -2.7
311,596 + +1.6
311,596 + +1.8
311,597 - +0.5
311,597 - +0.8
311,597 - +1.0
311,597 - +1.6
311,597 - +1.0
311,597 - +0.1
311,597 - +1.1
311,597 - +1.9
311,597 - +0.3
311,597 - +1.2
311,597 - +2.2
311,597 - +1.3
311,597 - +1.4
311,963 - Psyr_0293 0.24 +0.5
312,251 - Psyr_0293 0.37 +2.6
312,362 + Psyr_0293 0.42 +0.8
312,363 - Psyr_0293 0.42 +0.8
312,445 + Psyr_0293 0.45 +0.7
312,445 + Psyr_0293 0.45 +0.6
312,446 - Psyr_0293 0.45 +0.3
312,697 + Psyr_0293 0.57 +0.2
312,856 + Psyr_0293 0.64 -0.9
312,856 + Psyr_0293 0.64 +2.0
312,926 + Psyr_0293 0.67 +1.6
312,926 + Psyr_0293 0.67 +0.2
312,926 + Psyr_0293 0.67 +0.9
312,927 - Psyr_0293 0.67 +1.5
312,927 - Psyr_0293 0.67 +1.1
312,927 - Psyr_0293 0.67 +1.1
312,927 - Psyr_0293 0.67 +0.6
312,927 - Psyr_0293 0.67 +0.3
312,927 - Psyr_0293 0.67 +1.0
312,927 - Psyr_0293 0.67 +0.2
312,927 - Psyr_0293 0.67 -0.9
312,927 - Psyr_0293 0.67 +2.2
312,955 + Psyr_0293 0.69 +0.7
312,955 + Psyr_0293 0.69 +0.9
312,956 - Psyr_0293 0.69 +2.3
312,956 - Psyr_0293 0.69 -0.9
312,956 - Psyr_0293 0.69 +0.4
312,979 + Psyr_0293 0.70 +1.5
312,979 + Psyr_0293 0.70 +2.0
312,979 + Psyr_0293 0.70 +1.0
312,979 + Psyr_0293 0.70 +1.1
312,979 + Psyr_0293 0.70 +1.6
312,980 - Psyr_0293 0.70 +0.8
312,980 - Psyr_0293 0.70 +2.8
312,980 - Psyr_0293 0.70 +1.0
312,980 - Psyr_0293 0.70 +1.3
312,980 - Psyr_0293 0.70 +1.2
312,989 - Psyr_0293 0.70 +0.7
313,225 + Psyr_0293 0.81 +3.3
313,225 + Psyr_0293 0.81 +1.9
313,225 + Psyr_0293 0.81 +1.1
313,225 + Psyr_0293 0.81 +1.4
313,225 + Psyr_0293 0.81 +1.8
313,226 - Psyr_0293 0.81 +1.7
313,226 - Psyr_0293 0.81 +1.0
313,226 - Psyr_0293 0.81 -0.3
313,226 - Psyr_0293 0.81 +1.5
313,366 + Psyr_0293 0.87 +0.7
313,366 + Psyr_0293 0.87 +2.0
313,366 + Psyr_0293 0.87 +1.8
313,366 + Psyr_0293 0.87 +1.4
313,366 + Psyr_0293 0.87 -0.1
313,366 + Psyr_0293 0.87 +1.5
313,366 + Psyr_0293 0.87 +0.7
313,366 + Psyr_0293 0.87 +1.3
313,367 - Psyr_0293 0.87 +0.7
313,367 - Psyr_0293 0.87 +1.2
313,367 - Psyr_0293 0.87 +1.4
313,367 - Psyr_0293 0.87 +0.3
313,507 + +3.2
313,508 - +1.5
313,508 - +0.0
313,643 + -1.2
313,643 + +0.2
313,900 + Psyr_0294 0.17 -1.6
313,984 - Psyr_0294 0.22 -2.9
314,008 - Psyr_0294 0.23 +0.4
314,067 + Psyr_0294 0.27 -1.2
314,164 - Psyr_0294 0.33 -0.1
314,198 + Psyr_0294 0.35 -1.9
314,198 + Psyr_0294 0.35 -3.0
314,198 + Psyr_0294 0.35 -1.2
314,198 + Psyr_0294 0.35 -2.0
314,199 - Psyr_0294 0.36 -1.4
314,199 - Psyr_0294 0.36 -2.2
314,199 - Psyr_0294 0.36 -0.9
314,199 - Psyr_0294 0.36 +0.6
314,233 - Psyr_0294 0.38 -3.6
314,568 + Psyr_0294 0.59 -1.9
314,569 - Psyr_0294 0.59 -2.9
314,569 - Psyr_0294 0.59 -2.3
314,580 + Psyr_0294 0.60 +0.4
314,614 - Psyr_0294 0.62 +0.4

Or see this region's nucleotide sequence