Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0215 and Psyr_0216 are separated by 82 nucleotides Psyr_0216 and Psyr_0217 are separated by 10 nucleotides Psyr_0217 and Psyr_0218 are separated by 331 nucleotides
Psyr_0215: Psyr_0215 - Exodeoxyribonuclease III xth, at 228,597 to 229,376
_0215
Psyr_0216: Psyr_0216 - orotate phosphoribosyltransferase, at 229,459 to 230,100
_0216
Psyr_0217: Psyr_0217 - conserved hypothetical protein, at 230,111 to 230,743
_0217
Psyr_0218: Psyr_0218 - N-acetylglutamate kinase, at 231,075 to 231,980
_0218
Position (kb) 230
231 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 229.166 kb on - strand, within Psyr_0215 at 229.166 kb on - strand, within Psyr_0215 at 229.210 kb on + strand, within Psyr_0215 at 229.210 kb on + strand, within Psyr_0215 at 229.211 kb on - strand, within Psyr_0215 at 229.219 kb on + strand, within Psyr_0215 at 229.219 kb on + strand, within Psyr_0215 at 229.219 kb on + strand, within Psyr_0215 at 229.220 kb on - strand, within Psyr_0215 at 229.220 kb on - strand, within Psyr_0215 at 229.278 kb on + strand, within Psyr_0215 at 229.278 kb on + strand, within Psyr_0215 at 229.353 kb on + strand at 229.353 kb on + strand at 229.354 kb on - strand at 229.354 kb on - strand at 229.354 kb on - strand at 229.354 kb on - strand at 229.376 kb on + strand at 229.403 kb on + strand at 229.403 kb on + strand at 229.403 kb on + strand at 229.403 kb on + strand at 229.404 kb on - strand at 229.404 kb on - strand at 229.420 kb on - strand at 229.423 kb on + strand at 229.423 kb on + strand at 229.423 kb on + strand at 229.423 kb on + strand at 229.423 kb on + strand at 229.427 kb on + strand at 229.427 kb on + strand at 229.428 kb on - strand at 229.442 kb on + strand at 229.672 kb on + strand, within Psyr_0216 at 230.238 kb on - strand, within Psyr_0217 at 230.297 kb on + strand, within Psyr_0217 at 230.297 kb on + strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.313 kb on - strand, within Psyr_0217 at 230.365 kb on + strand, within Psyr_0217 at 230.438 kb on + strand, within Psyr_0217 at 230.439 kb on - strand, within Psyr_0217 at 230.839 kb on + strand at 231.043 kb on - strand at 231.065 kb on + strand at 231.066 kb on - strand at 231.109 kb on - strand at 231.109 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 229,166 - Psyr_0215 0.73 -3.4 229,166 - Psyr_0215 0.73 -1.5 229,210 + Psyr_0215 0.79 -2.9 229,210 + Psyr_0215 0.79 +0.1 229,211 - Psyr_0215 0.79 -0.6 229,219 + Psyr_0215 0.80 -1.0 229,219 + Psyr_0215 0.80 -3.9 229,219 + Psyr_0215 0.80 -1.9 229,220 - Psyr_0215 0.80 -5.2 229,220 - Psyr_0215 0.80 -1.5 229,278 + Psyr_0215 0.87 -3.0 229,278 + Psyr_0215 0.87 -1.7 229,353 + -0.9 229,353 + -4.2 229,354 - -3.2 229,354 - -3.9 229,354 - -3.5 229,354 - -3.8 229,376 + -2.9 229,403 + -3.3 229,403 + -2.2 229,403 + -3.0 229,403 + -1.4 229,404 - -0.5 229,404 - +1.8 229,420 - +1.5 229,423 + -3.6 229,423 + -2.2 229,423 + -1.0 229,423 + -3.1 229,423 + -2.6 229,427 + -4.0 229,427 + -2.8 229,428 - -2.5 229,442 + -0.4 229,672 + Psyr_0216 0.33 -0.5 230,238 - Psyr_0217 0.20 -0.1 230,297 + Psyr_0217 0.29 +1.0 230,297 + Psyr_0217 0.29 -0.0 230,298 - Psyr_0217 0.30 -0.1 230,298 - Psyr_0217 0.30 +0.0 230,298 - Psyr_0217 0.30 -0.3 230,298 - Psyr_0217 0.30 -1.2 230,313 - Psyr_0217 0.32 +0.9 230,365 + Psyr_0217 0.40 +0.6 230,438 + Psyr_0217 0.52 -0.0 230,439 - Psyr_0217 0.52 -0.3 230,839 + +0.5 231,043 - -0.9 231,065 + -0.5 231,066 - +0.5 231,109 - +1.5 231,109 - +2.0
Or see this region's nucleotide sequence