Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0135 and Psyr_0136 are separated by 53 nucleotides Psyr_0136 and Psyr_0137 overlap by 1 nucleotides Psyr_0137 and Psyr_0138 are separated by 210 nucleotides
Psyr_0135: Psyr_0135 - outer membrane transport energization protein TonB, at 144,401 to 145,225
_0135
Psyr_0136: Psyr_0136 - outer membrane transport energization protein ExbB, at 145,279 to 145,986
_0136
Psyr_0137: Psyr_0137 - outer membrane transport energization protein ExbD, at 145,986 to 146,387
_0137
Psyr_0138: Psyr_0138 - TonB-dependent receptor:TonB-dependent receptor, at 146,598 to 149,312
_0138
Position (kb)
145
146
147 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 144.993 kb on - strand, within Psyr_0135 at 145.180 kb on - strand at 145.255 kb on - strand at 145.273 kb on + strand at 145.273 kb on + strand at 145.274 kb on - strand at 145.343 kb on + strand at 145.381 kb on + strand, within Psyr_0136 at 145.382 kb on - strand, within Psyr_0136 at 145.382 kb on - strand, within Psyr_0136 at 145.382 kb on - strand, within Psyr_0136 at 145.382 kb on - strand, within Psyr_0136 at 145.382 kb on - strand, within Psyr_0136 at 145.382 kb on - strand, within Psyr_0136 at 145.472 kb on - strand, within Psyr_0136 at 145.491 kb on + strand, within Psyr_0136 at 145.607 kb on + strand, within Psyr_0136 at 145.607 kb on + strand, within Psyr_0136 at 145.608 kb on - strand, within Psyr_0136 at 145.834 kb on + strand, within Psyr_0136 at 145.834 kb on + strand, within Psyr_0136 at 145.835 kb on - strand, within Psyr_0136 at 145.835 kb on - strand, within Psyr_0136 at 145.835 kb on - strand, within Psyr_0136 at 145.984 kb on + strand at 145.984 kb on + strand at 145.985 kb on - strand at 146.199 kb on + strand, within Psyr_0137 at 146.199 kb on + strand, within Psyr_0137 at 146.366 kb on - strand at 146.587 kb on + strand at 146.788 kb on + strand at 146.788 kb on + strand at 146.788 kb on + strand at 146.788 kb on + strand at 147.156 kb on + strand, within Psyr_0138 at 147.156 kb on + strand, within Psyr_0138 at 147.157 kb on - strand, within Psyr_0138 at 147.186 kb on - strand, within Psyr_0138 at 147.186 kb on - strand, within Psyr_0138 at 147.186 kb on - strand, within Psyr_0138 at 147.320 kb on + strand, within Psyr_0138 at 147.342 kb on + strand, within Psyr_0138 at 147.342 kb on + strand, within Psyr_0138 at 147.342 kb on + strand, within Psyr_0138 at 147.342 kb on + strand, within Psyr_0138 at 147.342 kb on + strand, within Psyr_0138 at 147.343 kb on - strand, within Psyr_0138 at 147.343 kb on - strand, within Psyr_0138 at 147.343 kb on - strand, within Psyr_0138
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 144,993 - Psyr_0135 0.72 +0.1 145,180 - -0.3 145,255 - +1.6 145,273 + -0.1 145,273 + +0.0 145,274 - -1.0 145,343 + +0.5 145,381 + Psyr_0136 0.14 +0.7 145,382 - Psyr_0136 0.15 -0.1 145,382 - Psyr_0136 0.15 +0.3 145,382 - Psyr_0136 0.15 -0.1 145,382 - Psyr_0136 0.15 -0.4 145,382 - Psyr_0136 0.15 +1.5 145,382 - Psyr_0136 0.15 +0.1 145,472 - Psyr_0136 0.27 -1.1 145,491 + Psyr_0136 0.30 +0.0 145,607 + Psyr_0136 0.46 -1.1 145,607 + Psyr_0136 0.46 -0.3 145,608 - Psyr_0136 0.46 -0.4 145,834 + Psyr_0136 0.78 +1.1 145,834 + Psyr_0136 0.78 -1.6 145,835 - Psyr_0136 0.79 -0.3 145,835 - Psyr_0136 0.79 +0.1 145,835 - Psyr_0136 0.79 +0.2 145,984 + -1.4 145,984 + -1.5 145,985 - -0.2 146,199 + Psyr_0137 0.53 -2.1 146,199 + Psyr_0137 0.53 -0.7 146,366 - +1.9 146,587 + -1.2 146,788 + -0.9 146,788 + +0.3 146,788 + -1.6 146,788 + -0.3 147,156 + Psyr_0138 0.21 -0.2 147,156 + Psyr_0138 0.21 +0.1 147,157 - Psyr_0138 0.21 +0.3 147,186 - Psyr_0138 0.22 +0.1 147,186 - Psyr_0138 0.22 -0.1 147,186 - Psyr_0138 0.22 +0.5 147,320 + Psyr_0138 0.27 +0.2 147,342 + Psyr_0138 0.27 -0.9 147,342 + Psyr_0138 0.27 -1.8 147,342 + Psyr_0138 0.27 +0.1 147,342 + Psyr_0138 0.27 -0.7 147,342 + Psyr_0138 0.27 -0.6 147,343 - Psyr_0138 0.27 -0.8 147,343 - Psyr_0138 0.27 +0.4 147,343 - Psyr_0138 0.27 -0.2
Or see this region's nucleotide sequence