Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0012 and Psyr_0013 are separated by 20 nucleotides Psyr_0013 and Psyr_0014 are separated by 38 nucleotides Psyr_0014 and Psyr_0015 are separated by 339 nucleotides
Psyr_0012: Psyr_0012 - glycyl-tRNA synthetase alpha chain, at 13,967 to 14,974
_0012
Psyr_0013: Psyr_0013 - DNA-3-methyladenine glycosylase I, at 14,995 to 15,546
_0013
Psyr_0014: Psyr_0014 - lipid A biosynthesis acyltransferase, at 15,585 to 16,472
_0014
Psyr_0015: Psyr_0015 - Oxidoreductase alpha (molybdopterin) subunit, at 16,812 to 19,175
_0015
Position (kb) 15
16
17 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 14.956 kb on - strand at 14.956 kb on - strand at 14.994 kb on + strand at 15.031 kb on + strand at 15.031 kb on + strand at 15.031 kb on + strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.040 kb on + strand at 15.040 kb on + strand at 15.040 kb on + strand at 15.040 kb on + strand at 15.041 kb on - strand at 15.041 kb on - strand at 15.170 kb on + strand, within Psyr_0013 at 15.171 kb on - strand, within Psyr_0013 at 15.171 kb on - strand, within Psyr_0013 at 15.171 kb on - strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.476 kb on - strand, within Psyr_0013 at 15.476 kb on - strand, within Psyr_0013 at 15.591 kb on - strand at 15.975 kb on + strand, within Psyr_0014 at 15.975 kb on + strand, within Psyr_0014 at 16.302 kb on - strand, within Psyr_0014 at 16.302 kb on - strand, within Psyr_0014 at 16.302 kb on - strand, within Psyr_0014 at 16.335 kb on + strand, within Psyr_0014 at 16.335 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.389 kb on + strand at 16.389 kb on + strand at 16.413 kb on + strand at 16.413 kb on + strand at 16.413 kb on + strand at 16.413 kb on + strand at 16.414 kb on - strand at 16.414 kb on - strand at 16.467 kb on + strand at 16.467 kb on + strand at 16.470 kb on + strand at 16.471 kb on - strand at 16.471 kb on - strand at 16.471 kb on - strand at 16.550 kb on - strand at 16.550 kb on - strand at 16.550 kb on - strand at 16.550 kb on - strand at 16.631 kb on + strand at 16.631 kb on + strand at 16.632 kb on - strand at 16.683 kb on - strand at 16.701 kb on + strand at 16.701 kb on + strand at 16.701 kb on + strand at 16.701 kb on + strand at 16.702 kb on - strand at 16.702 kb on - strand at 16.702 kb on - strand at 16.778 kb on + strand at 16.778 kb on + strand at 16.779 kb on - strand at 16.913 kb on + strand at 16.945 kb on - strand at 16.967 kb on - strand at 16.970 kb on - strand at 17.068 kb on + strand, within Psyr_0015 at 17.068 kb on + strand, within Psyr_0015 at 17.069 kb on - strand, within Psyr_0015 at 17.069 kb on - strand, within Psyr_0015 at 17.167 kb on + strand, within Psyr_0015 at 17.167 kb on + strand, within Psyr_0015 at 17.167 kb on + strand, within Psyr_0015 at 17.167 kb on + strand, within Psyr_0015 at 17.251 kb on + strand, within Psyr_0015 at 17.251 kb on + strand, within Psyr_0015 at 17.251 kb on + strand, within Psyr_0015 at 17.252 kb on - strand, within Psyr_0015 at 17.252 kb on - strand, within Psyr_0015 at 17.252 kb on - strand, within Psyr_0015 at 17.252 kb on - strand, within Psyr_0015 at 17.255 kb on + strand, within Psyr_0015 at 17.255 kb on + strand, within Psyr_0015 at 17.255 kb on + strand, within Psyr_0015 at 17.255 kb on + strand, within Psyr_0015 at 17.256 kb on - strand, within Psyr_0015 at 17.256 kb on - strand, within Psyr_0015 at 17.256 kb on - strand, within Psyr_0015 at 17.256 kb on - strand, within Psyr_0015 at 17.256 kb on - strand, within Psyr_0015 at 17.256 kb on - strand, within Psyr_0015 at 17.256 kb on - strand, within Psyr_0015 at 17.256 kb on - strand, within Psyr_0015 at 17.368 kb on + strand, within Psyr_0015 at 17.368 kb on + strand, within Psyr_0015 at 17.385 kb on + strand, within Psyr_0015 at 17.385 kb on + strand, within Psyr_0015 at 17.386 kb on - strand, within Psyr_0015 at 17.416 kb on + strand, within Psyr_0015 at 17.417 kb on - strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 14,956 - +0.3 14,956 - +0.6 14,994 + +0.1 15,031 + +0.1 15,031 + +0.5 15,031 + +0.5 15,032 - +0.5 15,032 - +0.4 15,032 - -0.3 15,032 - -0.2 15,032 - +0.5 15,032 - +0.9 15,032 - -0.8 15,032 - +3.9 15,032 - -0.1 15,040 + -0.4 15,040 + +0.9 15,040 + +0.1 15,040 + -0.3 15,041 - +0.1 15,041 - -0.4 15,170 + Psyr_0013 0.32 -0.4 15,171 - Psyr_0013 0.32 +0.3 15,171 - Psyr_0013 0.32 +0.5 15,171 - Psyr_0013 0.32 -1.0 15,475 + Psyr_0013 0.87 -0.8 15,475 + Psyr_0013 0.87 +0.8 15,475 + Psyr_0013 0.87 +0.0 15,475 + Psyr_0013 0.87 +0.2 15,475 + Psyr_0013 0.87 -0.3 15,475 + Psyr_0013 0.87 +0.7 15,475 + Psyr_0013 0.87 -1.2 15,476 - Psyr_0013 0.87 -1.3 15,476 - Psyr_0013 0.87 +0.7 15,591 - -1.9 15,975 + Psyr_0014 0.44 -2.3 15,975 + Psyr_0014 0.44 -4.3 16,302 - Psyr_0014 0.81 -3.0 16,302 - Psyr_0014 0.81 -1.1 16,302 - Psyr_0014 0.81 +0.9 16,335 + Psyr_0014 0.84 +0.3 16,335 - Psyr_0014 0.84 -3.0 16,336 - Psyr_0014 0.85 -1.5 16,336 - Psyr_0014 0.85 -3.1 16,336 - Psyr_0014 0.85 -0.1 16,336 - Psyr_0014 0.85 -0.8 16,336 - Psyr_0014 0.85 -2.8 16,336 - Psyr_0014 0.85 -2.6 16,389 + +0.8 16,389 + -1.3 16,413 + +1.5 16,413 + -2.6 16,413 + -2.0 16,413 + -3.2 16,414 - -2.0 16,414 - -3.1 16,467 + -2.2 16,467 + -0.2 16,470 + -1.3 16,471 - -0.9 16,471 - +0.2 16,471 - -0.1 16,550 - +1.2 16,550 - -1.0 16,550 - -0.9 16,550 - -0.5 16,631 + +0.4 16,631 + +0.8 16,632 - +0.7 16,683 - -2.4 16,701 + +0.4 16,701 + -0.2 16,701 + +0.3 16,701 + -0.3 16,702 - +0.7 16,702 - -0.1 16,702 - +0.3 16,778 + +1.1 16,778 + -0.5 16,779 - +0.7 16,913 + +2.3 16,945 - +0.5 16,967 - +0.3 16,970 - -0.3 17,068 + Psyr_0015 0.11 +0.3 17,068 + Psyr_0015 0.11 +0.3 17,069 - Psyr_0015 0.11 -0.5 17,069 - Psyr_0015 0.11 +0.6 17,167 + Psyr_0015 0.15 -0.9 17,167 + Psyr_0015 0.15 -0.6 17,167 + Psyr_0015 0.15 -0.8 17,167 + Psyr_0015 0.15 +0.3 17,251 + Psyr_0015 0.19 +0.7 17,251 + Psyr_0015 0.19 +0.4 17,251 + Psyr_0015 0.19 +0.2 17,252 - Psyr_0015 0.19 -0.1 17,252 - Psyr_0015 0.19 +1.0 17,252 - Psyr_0015 0.19 -0.2 17,252 - Psyr_0015 0.19 +0.2 17,255 + Psyr_0015 0.19 +0.1 17,255 + Psyr_0015 0.19 +0.4 17,255 + Psyr_0015 0.19 +1.2 17,255 + Psyr_0015 0.19 +0.4 17,256 - Psyr_0015 0.19 -0.6 17,256 - Psyr_0015 0.19 -0.5 17,256 - Psyr_0015 0.19 -0.1 17,256 - Psyr_0015 0.19 +3.1 17,256 - Psyr_0015 0.19 -0.1 17,256 - Psyr_0015 0.19 +0.0 17,256 - Psyr_0015 0.19 -0.2 17,256 - Psyr_0015 0.19 +0.7 17,368 + Psyr_0015 0.24 -0.3 17,368 + Psyr_0015 0.24 -0.4 17,385 + Psyr_0015 0.24 +1.3 17,385 + Psyr_0015 0.24 -0.5 17,386 - Psyr_0015 0.24 -0.5 17,416 + Psyr_0015 0.26 +0.2 17,417 - Psyr_0015 0.26 -0.1 17,428 + Psyr_0015 0.26 +0.3 17,428 + Psyr_0015 0.26 +0.9 17,428 + Psyr_0015 0.26 -0.5 17,428 + Psyr_0015 0.26 +1.2 17,428 + Psyr_0015 0.26 -0.8 17,428 + Psyr_0015 0.26 -0.8 17,428 + Psyr_0015 0.26 +0.7 17,428 + Psyr_0015 0.26 -0.7 17,428 + Psyr_0015 0.26 +0.5 17,428 + Psyr_0015 0.26 +0.1 17,428 + Psyr_0015 0.26 +0.4 17,428 + Psyr_0015 0.26 -0.7 17,428 + Psyr_0015 0.26 -2.8 17,428 + Psyr_0015 0.26 +2.5 17,428 + Psyr_0015 0.26 +1.9 17,428 + Psyr_0015 0.26 -0.9 17,428 + Psyr_0015 0.26 -1.1 17,428 + Psyr_0015 0.26 -0.7 17,428 + Psyr_0015 0.26 -0.5 17,428 + Psyr_0015 0.26 -0.9 17,428 + Psyr_0015 0.26 +0.6 17,428 + Psyr_0015 0.26 -0.5 17,428 + Psyr_0015 0.26 -1.7 17,428 + Psyr_0015 0.26 +0.3 17,428 + Psyr_0015 0.26 -0.7 17,428 + Psyr_0015 0.26 +0.8 17,428 + Psyr_0015 0.26 +0.6 17,428 + Psyr_0015 0.26 -0.4 17,428 + Psyr_0015 0.26 +1.5 17,428 + Psyr_0015 0.26 +0.5 17,429 - Psyr_0015 0.26 +0.1 17,429 - Psyr_0015 0.26 -0.6 17,429 - Psyr_0015 0.26 +0.1 17,429 - Psyr_0015 0.26 -2.1 17,429 - Psyr_0015 0.26 -0.3 17,429 - Psyr_0015 0.26 +0.9 17,429 - Psyr_0015 0.26 +0.5 17,429 - Psyr_0015 0.26 +0.1 17,429 - Psyr_0015 0.26 -0.5 17,429 - Psyr_0015 0.26 -1.4 17,429 - Psyr_0015 0.26 -0.6 17,429 - Psyr_0015 0.26 +0.0 17,429 - Psyr_0015 0.26 +0.6 17,429 - Psyr_0015 0.26 +0.3 17,429 - Psyr_0015 0.26 +0.9 17,429 - Psyr_0015 0.26 -1.2 17,429 - Psyr_0015 0.26 -1.1 17,429 - Psyr_0015 0.26 +1.1 17,429 - Psyr_0015 0.26 +0.4 17,429 - Psyr_0015 0.26 +0.4 17,429 - Psyr_0015 0.26 +0.8 17,429 - Psyr_0015 0.26 -0.8 17,429 - Psyr_0015 0.26 -0.5 17,429 - Psyr_0015 0.26 -2.7
Or see this region's nucleotide sequence