Experiment: Varel_Bryant_medium_Glucose with Chenodeoxycholic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2003 and BT2004 are separated by 12 nucleotides BT2004 and BT2005 overlap by 4 nucleotides BT2005 and BT2006 are separated by 129 nucleotides BT2006 and BT2007 are separated by 121 nucleotides
BT2003: BT2003 - putative membrane peptidase (NCBI ptt file), at 2,519,858 to 2,520,715
BT2003
BT2004: BT2004 - RimM protein, required for 16S rRNA processing (NCBI ptt file), at 2,520,728 to 2,521,267
BT2004
BT2005: BT2005 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (NCBI ptt file), at 2,521,264 to 2,522,568
BT2005
BT2006: BT2006 - hypothetical protein (NCBI ptt file), at 2,522,698 to 2,523,312
BT2006
BT2007: BT2007 - conserved hypothetical protein, putative glycoprotease (NCBI ptt file), at 2,523,434 to 2,524,129
BT2007
Position (kb)
2521
2522
2523 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2520.641 kb on - strand at 2522.594 kb on - strand at 2522.597 kb on - strand at 2522.618 kb on - strand at 2522.681 kb on - strand at 2523.169 kb on - strand, within BT2006 at 2523.169 kb on - strand, within BT2006 at 2523.169 kb on - strand, within BT2006 at 2523.196 kb on - strand, within BT2006 at 2523.257 kb on - strand at 2523.306 kb on - strand at 2523.405 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Chenodeoxycholic 0.25 mg/ml remove 2,520,641 - -3.2 2,522,594 - -1.4 2,522,597 - -2.9 2,522,618 - -2.3 2,522,681 - -2.0 2,523,169 - BT2006 0.77 -0.1 2,523,169 - BT2006 0.77 +0.8 2,523,169 - BT2006 0.77 -0.9 2,523,196 - BT2006 0.81 +0.6 2,523,257 - -2.2 2,523,306 - -0.4 2,523,405 - -2.9
Or see this region's nucleotide sequence